| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 7, 5403-5411, February 16, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Endocrinologie, Métabolisme et Développement,
CNRS-UPR 1524, 9 Rue J. Hetzel, 92190 Meudon, France
We have previously shown that the first 147 N-terminal residues of the rat liver carnitine palmitoyltransferase 1 (CPT1), encompassing its two transmembrane (TM) segments, specify both mitochondrial targeting and anchorage at the outer mitochondrial membrane (OMM). In the present study, we have identified the precise import sequence in this polytopic OMM protein. In vitro
import studies with fusion and deletion CPT1 proteins demonstrated that none of its TM segments behave as a signal anchor sequence. Analysis of
the regions flanking the TM segments revealed that residues 123-147,
located immediately downstream of TM2, function as a noncleavable,
matrix-targeting signal. They specify mitochondrial targeting, whereas
the hydrophobic TM segment(s) acts as a stop-transfer sequence that
stops and anchors the translocating CPT1 into the OMM. Heterologous
expression in Saccharomyces cerevisiae of several deleted
CPT1 proteins not only confirms the validity of the "stop-transfer" import model but also indicates that residues 1-82 of CPT1 contain a
putative microsomal targeting signal whose cellular significance awaits
further investigation. Finally, we identified a highly folded core
within the C-terminal domain of CPT1 that is hidden in the entire
protein by its cytosolic N-terminal residues. Functional analysis of
the deleted CPT1 proteins indicates that this folded C-terminal core,
which may belong to the catalytic domain of CPT1, requires TM2 for its
correct folding achievement and is in close proximity to residues
1-47.
The N-terminal Domain of Rat Liver Carnitine Palmitoyltransferase
1 Contains an Internal Mitochondrial Import Signal and Residues
Essential for Folding of Its C-terminal Catalytic Domain*
,
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Recipient of a doctoral fellowship from the Ministère de
l'Education Nationale, de la Recherche et de la Technologie, and of
the Fondation pour la Recherche Médicale.
§
To whom correspondence should be addressed. Tel.: 33 1 45 07 51 68;
Fax: 33 1 45 07 50 39; E-mail: pripbuus@infobiogen.fr.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Y. Sierra, E. Gratacos, P. Carrasco, J. Clotet, J. Urena, D. Serra, G. Asins, F. G. Hegardt, and N. Casals CPT1c Is Localized in Endoplasmic Reticulum of Neurons and Has Carnitine Palmitoyltransferase Activity J. Biol. Chem., March 14, 2008; 283(11): 6878 - 6885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Faye, C. Esnous, N. T. Price, M. A. Onfray, J. Girard, and C. Prip-Buus Rat Liver Carnitine Palmitoyltransferase 1 Forms an Oligomeric Complex within the Outer Mitochondrial Membrane J. Biol. Chem., September 14, 2007; 282(37): 26908 - 26916. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Valentin, P. Dunoyer, G. Vetter, C. Schalk, A. Dietrich, and S. Bouzoubaa Molecular Basis for Mitochondrial Localization of Viral Particles during Beet Necrotic Yellow Vein Virus Infection J. Virol., August 1, 2005; 79(15): 9991 - 10002. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Guais, B. Solhonne, N. Melaine, G. Guellaen, and F. Bulle Goliath, a Ring-H2 Mitochondrial Protein, Regulated by Luteinizing Hormone/Human Chorionic Gonadotropin in Rat Leydig Cells Biol Reprod, January 1, 2004; 70(1): 204 - 213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gobin, L. Thuillier, G. Jogl, A. Faye, L. Tong, M. Chi, J.-P. Bonnefont, J. Girard, and C. Prip-Buus Functional and Structural Basis of Carnitine Palmitoyltransferase 1A Deficiency J. Biol. Chem., December 12, 2003; 278(50): 50428 - 50434. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Pan, I. Cohen, F. Guillerault, B. Feve, J. Girard, and C. Prip-Buus The Extreme C Terminus of Rat Liver Carnitine Palmitoyltransferase I Is Not Involved in Malonyl-CoA Sensitivity but in Initial Protein Folding J. Biol. Chem., November 27, 2002; 277(49): 47184 - 47189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Weber-Lotfi, A. Dietrich, M. Russo, and L. Rubino Mitochondrial Targeting and Membrane Anchoring of a Viral Replicase in Plant and Yeast Cells J. Virol., September 11, 2002; 76(20): 10485 - 10496. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. R. van der Leij, K. B. Cox, V. N. Jackson, N. C. A. Huijkman, B. Bartelds, J. R. G. Kuipers, T. Dijkhuizen, P. Terpstra, P. A. Wood, V. A. Zammit, et al. Structural and Functional Genomics of the CPT1B Gene for Muscle-type Carnitine Palmitoyltransferase I in Mammals J. Biol. Chem., July 19, 2002; 277(30): 26994 - 27005. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
