| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 8, 5417-5420, February 23, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
,
From the Cold Spring Harbor Laboratory, Cold Spring
Harbor, New York 11724
13S condensin is a five-subunit protein
complex that plays a central role in mitotic chromosome condensation.
The condensin complex was originally identified and purified from
Xenopus egg extracts and shown to have an
ATP-dependent positive supercoiling activity in
vitro. We report here the characterization of a human condensin
complex purified from HeLa cell nuclear extracts. The human 13S complex
has exactly the same composition as its Xenopus counterpart, being composed of two structural maintenance of
chromosomes (human chromosome-associated polypeptide (hCAP)-C
and hCAP-E) subunits and three non-structural maintenance of
chromosomes (hCAP-D2/CNAP1, hCAP-G, and hCAP-H/BRRN) subunits. Human
condensin purified from asynchronous HeLa cell cultures fails to
reconfigure DNA structure in vitro. When phosphorylated by
purified cdc2-cyclin B, however, it gains the ability to introduce
positive supercoils into DNA in the presence of ATP and topoisomerase
I. Strikingly, human condensin can induce chromosome condensation when
added back into a Xenopus egg extract that has been
immunodepleted of endogenous condensin. Thus, the structure and
function of the condensin complex are highly conserved between
Xenopus and humans, underscoring its fundamental importance
in mitotic chromosome dynamics in eukaryotic cells.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF331796.
Present address: Cellular Physiology Laboratory, The Institute of
Physical and Chemical Research (Riken), 2-1 Hirosawa, Wako, Saitama
351-01, Japan.
§
To whom correspondence should be addressed: Cold Spring Harbor
Laboratory, One Bungtown Rd., P. O. Box 100, Cold Spring Harbor, NY
11724. Tel.: 516-367-8370; Fax: 516-367-8815; E-mail:
hirano@cshl.org.
This article has been cited by other articles:
|
|
 |
|
  I. K. Darcy, R. G. Scharein, and A. Stasiak 3D visualization software to analyze topological outcomes of topoisomerase reactions Nucleic Acids Res., April 24, 2008; (2008) gkn192v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. D. Lavoie pRb and condensin--local control of global chromosome structure Genes & Dev., April 15, 2008; 22(8): 964 - 969. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Longworth, A. Herr, J.-Y. Ji, and N. J. Dyson RBF1 promotes chromatin condensation through a conserved interaction with the Condensin II protein dCAP-D3 Genes & Dev., April 15, 2008; 22(8): 1011 - 1024. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-P. Lee, J.-Y. Chen, J.-T. Wang, K. Kimura, A. Takemoto, C.-C. Lu, and M.-R. Chen Epstein-Barr Virus BGLF4 Kinase Induces Premature Chromosome Condensation through Activation of Condensin and Topoisomerase II J. Virol., May 15, 2007; 81(10): 5166 - 5180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Huster, T. D. Purnat, J. L. Burkhead, M. Ralle, O. Fiehn, F. Stuckert, N. E. Olson, D. Teupser, and S. Lutsenko High Copper Selectively Alters Lipid Metabolism and Cell Cycle Machinery in the Mouse Model of Wilson Disease J. Biol. Chem., March 16, 2007; 282(11): 8343 - 8355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. W. Lam, E. A. Peterson, M. Yeung, and B. D. Lavoie Condensin is required for chromosome arm cohesion during mitosis. Genes & Dev., November 1, 2006; 20(21): 2973 - 2984. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Blank, Y. Lerenthal, L. Mittelman, and Y. Shiloh Condensin I recruitment and uneven chromatin condensation precede mitotic cell death in response to DNA damage J. Cell Biol., July 17, 2006; 174(2): 195 - 206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. M. Petrushenko, C.-H. Lai, R. Rai, and V. V. Rybenkov DNA Reshaping by MukB RIGHT-HANDED KNOTTING, LEFT-HANDED SUPERCOILING J. Biol. Chem., February 24, 2006; 281(8): 4606 - 4615. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Verlinden, G. Eelen, I. Beullens, M. Van Camp, P. Van Hummelen, K. Engelen, R. Van Hellemont, K. Marchal, B. De Moor, F. Foijer, et al. Characterization of the Condensin Component Cnap1 and Protein Kinase Melk as Novel E2F Target Genes Down-regulated by 1,25-Dihydroxyvitamin D3 J. Biol. Chem., November 11, 2005; 280(45): 37319 - 37330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Stray, N. J. Crisona, B. P. Belotserkovskii, J. E. Lindsley, and N. R. Cozzarelli The Saccharomyces cerevisiae Smc2/4 Condensin Compacts DNA into (+) Chiral Structures without Net Supercoiling J. Biol. Chem., October 14, 2005; 280(41): 34723 - 34734. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B.-D. Wang, D. Eyre, M. Basrai, M. Lichten, and A. Strunnikov Condensin Binding at Distinct and Specific Chromosomal Sites in the Saccharomyces cerevisiae Genome Mol. Cell. Biol., August 15, 2005; 25(16): 7216 - 7225. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Uchiyama, S. Kobayashi, H. Takata, T. Ishihara, N. Hori, T. Higashi, K. Hayashihara, T. Sone, D. Higo, T. Nirasawa, et al. Proteome Analysis of Human Metaphase Chromosomes J. Biol. Chem., April 29, 2005; 280(17): 16994 - 17004. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Xing, D. C. Wilkerson, C. N. Mayhew, E. J. Lubert, H. S. Skaggs, M. L. Goodson, Y. Hong, O.-K. Park-Sarge, and K. D. Sarge Mechanism of hsp70i Gene Bookmarking Science, January 21, 2005; 307(5708): 421 - 423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Li, G. Sakashita, H. Matsuzaki, K. Sugimoto, K. Kimura, F. Hanaoka, H. Taniguchi, K. Furukawa, and T. Urano Direct Association with Inner Centromere Protein (INCENP) Activates the Novel Chromosomal Passenger Protein, Aurora-C J. Biol. Chem., November 5, 2004; 279(45): 47201 - 47211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kireeva, M. Lakonishok, I. Kireev, T. Hirano, and A. S. Belmont Visualization of early chromosome condensation: a hierarchical folding, axial glue model of chromosome structure J. Cell Biol., September 13, 2004; 166(6): 775 - 785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. B. Case, Y.-P. Chang, S. B. Smith, J. Gore, N. R. Cozzarelli, and C. Bustamante The Bacterial Condensin MukBEF Compacts DNA into a Repetitive, Stable Structure Science, July 9, 2004; 305(5681): 222 - 227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ono, Y. Fang, D. L. Spector, and T. Hirano Spatial and Temporal Regulation of Condensins I and II in Mitotic Chromosome Assembly in Human Cells Mol. Biol. Cell, July 1, 2004; 15(7): 3296 - 3308. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. S. Chen, T. Sutani, and M. Yanagida Cti1/C1D interacts with condensin SMC hinge and supports the DNA repair function of condensin PNAS, May 25, 2004; 101(21): 8078 - 8083. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Geiman, U. T. Sankpal, A. K. Robertson, Y. Chen, M. Mazumdar, J. T. Heale, J. A. Schmiesing, W. Kim, K. Yokomori, Y. Zhao, et al. Isolation and characterization of a novel DNA methyltransferase complex linking DNMT3B with components of the mitotic chromosome condensation machinery Nucleic Acids Res., May 17, 2004; 32(9): 2716 - 2729. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Takemoto, K. Kimura, S. Yokoyama, and F. Hanaoka Cell Cycle-dependent Phosphorylation, Nuclear Localization, and Activation of Human Condensin J. Biol. Chem., February 6, 2004; 279(6): 4551 - 4559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Coelho, J. Queiroz-Machado, and C. E. Sunkel Condensin-dependent localisation of topoisomerase II to an axial chromosomal structure is required for sister chromatid resolution during mitosis J. Cell Sci., December 1, 2003; 116(23): 4763 - 4776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Somma, B. Fasulo, G. Siriaco, and G. Cenci Chromosome Condensation Defects in barren RNA-Interfered Drosophila Cells Genetics, November 1, 2003; 165(3): 1607 - 1611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. U. Siddiqui, P. E. Stronghill, R. E. Dengler, C. A. Hasenkampf, and C. D. Riggs Mutations in Arabidopsis condensin genes disrupt embryogenesis, meristem organization and segregation of homologous chromosomes during meiosis Development, July 15, 2003; 130(14): 3283 - 3295. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Cuvier and T. Hirano A role of topoisomerase II in linking DNA replication to chromosome condensation J. Cell Biol., March 3, 2003; 160(5): 645 - 655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Przewloka, P. E. Pardington, S. M. Yannone, D. J. Chen, and R. B. Cary In Vitro and In Vivo Interactions of DNA Ligase IV with a Subunit of the Condensin Complex Mol. Biol. Cell, February 1, 2003; 14(2): 685 - 697. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Losada, M. Hirano, and T. Hirano Cohesin release is required for sister chromatid resolution, but not for condensin-mediated compaction, at the onset of mitosis Genes & Dev., December 1, 2002; 16(23): 3004 - 3016. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-S. Kim, T. B. Krasieva, V. LaMorte, A. M. R. Taylor, and K. Yokomori Specific Recruitment of Human Cohesin to Laser-induced DNA Damage J. Biol. Chem., November 15, 2002; 277(47): 45149 - 45153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Ball Jr., J. A. Schmiesing, C. Zhou, H. C. Gregson, Y. Okada, T. Doi, and K. Yokomori Identification of a Chromosome-Targeting Domain in the Human Condensin Subunit CNAP1/hCAP-D2/Eg7 Mol. Cell. Biol., August 15, 2002; 22(16): 5769 - 5781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bomar, P. Moreira, J. J. Balise, and P. Collas Differential regulation of maternal and paternal chromosome condensation in mitotic zygotes J. Cell Sci., July 15, 2002; 115(14): 2931 - 2940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Hagstrom, V. F. Holmes, N. R. Cozzarelli, and B. J. Meyer C. elegans condensin promotes mitotic chromosome architecture, centromere organization, and sister chromatid segregation during mitosis and meiosis Genes & Dev., March 15, 2002; 16(6): 729 - 742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. Lavoie, E. Hogan, and D. Koshland In vivo dissection of the chromosome condensation machinery: reversibility of condensation distinguishes contributions of condensin and cohesin J. Cell Biol., March 4, 2002; 156(5): 805 - 815. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hirano The ABCs of SMC proteins: two-armed ATPases for chromosome condensation, cohesion, and repair Genes & Dev., February 15, 2002; 16(4): 399 - 414. [Full Text] [PDF]
|
|
|
|
|
|
D. E. Anderson, A. Losada, H. P. Erickson, and T. Hirano Condensin and cohesin display different arm conformations with characteristic hinge angles J. Cell Biol., February 4, 2002; 156(3): 419 - 424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. A. Cabello, E. Eliseeva, W. He, H. Youssoufian, S. E. Plon, B. R. Brinkley, and J. W. Belmont Cell Cycle-dependent Expression and Nucleolar Localization of hCAP-H Mol. Biol. Cell, November 1, 2001; 12(11): 3527 - 3537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. Lavoie, E. Hogan, and D. Koshland In vivo dissection of the chromosome condensation machinery: reversibility of condensation distinguishes contributions of condensin and cohesin J. Cell Biol., March 4, 2002; 156(5): 805 - 815. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Anderson, A. Losada, H. P. Erickson, and T. Hirano Condensin and cohesin display different arm conformations with characteristic hinge angles J. Cell Biol., February 4, 2002; 156(3): 419 - 424. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
