|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 8, 5598-5604, February 23, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Institut de Biologie Moléculaire et Cellulaire, UPR
9002 du CNRS, 15 rue René Descartes, 67084 Strasbourg, France
Although their genomes cannot be aligned at the
nucleotide level, the HIV-1/SIVcpz and the HIV-2/SIVsm viruses are
closely related lentiviruses that contain homologous functional and
structural RNA elements in their 5'-untranslated regions. In both
groups, the domains containing the trans-activating region,
the 5'-copy of the polyadenylation signal, and the primer binding site
(PBS) are followed by a short stem-loop (SL1) containing a
six-nucleotide self-complementary sequence in the loop, flanked by
unpaired purines. In HIV-1, SL1 is involved in the dimerization of the
viral RNA, in vitro and in vivo. Here, we
tested whether SL1 has the same function in HIV-2 and SIVsm RNA.
Surprisingly, we found that SL1 is neither required nor involved in the
dimerization of HIV-2 and SIV RNA. We identified the NarI
sequence located in the PBS as the main site of HIV-2 RNA dimerization.
cis and trans complementation of point
mutations indicated that this self-complementary sequence forms
symmetrical intermolecular interactions in the RNA dimer and suggested
that HIV-2 and SIV RNA dimerization proceeds through a kissing loop
mechanism, as previously shown for HIV-1. Furthermore, annealing of
tRNA3Lys to the PBS strongly inhibited
in vitro RNA dimerization, indicating that, in
vivo, the intermolecular interaction involving the
NarI sequence must be dissociated to allow annealing of the
primer tRNA.
Identification of the in Vitro HIV-2/SIV RNA
Dimerization Site Reveals Striking Differences with HIV-1*
,§,
*
This work was supported by a grant from the Agence Nationale
de Recherche sur le SIDA (ANRS).The costs of publication of this article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Both authors contributed equally to this work.
§
Was a fellow of the ANRS. To whom correspondence may be
addressed. Present address: Division of Biological Sciences, The
University of Montana, Missoula, MT 59812. Tel.: 406-243-6393; Fax:
406-243-4304; E-mail: lodmell@selway.umt.edu.
¶
To whom correspondence may be addressed: Institut de Biologie
Moléculaire et Cellulaire, UPR 9002 du CNRS, 15 rue René
Descartes, 67084 Strasbourg, France. Tel.: 33-3-8841-7091; Fax:
33-3-8860-2218; E-mail: r.marquet@ibmc.u-strasbg.fr.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Centlivre, B. Klaver, B. Berkhout, and A. T. Das Functional Analysis of the Complex trans-Activating Response Element RNA Structure in Simian Immunodeficiency Virus J. Virol., September 15, 2008; 82(18): 9171 - 9178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. James and B. Sargueil RNA secondary structure of the feline immunodeficiency virus 5'UTR and Gag coding region Nucleic Acids Res., August 1, 2008; 36(14): 4653 - 4666. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. T. Baig, J.-M. Lanchy, and J. S. Lodmell HIV-2 RNA dimerization is regulated by intramolecular interactions in vitro RNA, August 1, 2007; 13(8): 1341 - 1354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-M. Lanchy, Q. N. Szafran, and J. S. Lodmell Splicing affects presentation of RNA dimerization signals in HIV-2 in vitro Nucleic Acids Res., August 27, 2004; 32(15): 4585 - 4595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-M. LANCHY, J. D. IVANOVITCH, and J. S. LODMELL A structural linkage between the dimerization and encapsidation signals in HIV-2 leader RNA RNA, August 1, 2003; 9(8): 1007 - 1018. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. G. Dirac, H. Huthoff, J. Kjems, and B. Berkhout Requirements for RNA heterodimerization of the human immunodeficiency virus type 1 (HIV-1) and HIV-2 genomes J. Gen. Virol., October 1, 2002; 83(10): 2533 - 2542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. G. Dirac, H. Huthoff, J. Kjems, and B. Berkhout Regulated HIV-2 RNA dimerization by means of alternative RNA conformations Nucleic Acids Res., June 15, 2002; 30(12): 2647 - 2655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ly and T. G. Parslow Bipartite Signal for Genomic RNA Dimerization in Moloney Murine Leukemia Virus J. Virol., March 7, 2002; 76(7): 3135 - 3144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. G. Dirac, H. Huthoff, J. Kjems, and B. Berkhout The Dimer Initiation Site Hairpin Mediates Dimerization of the Human Immunodeficiency Virus, Type 2 RNA Genome J. Biol. Chem., August 17, 2001; 276(34): 32345 - 32352. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |