Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M007524200 on November 28, 2000

J. Biol. Chem., Vol. 276, Issue 8, 5613-5621, February 23, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/8/5613    most recent
M007524200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Luo, D.
Right arrow Articles by Putney, J. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Luo, D.
Right arrow Articles by Putney, J. W., Jr.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Signaling Pathways Underlying Muscarinic Receptor-induced [Ca2+]i Oscillations in HEK293 Cells*

Dali LuoDagger , Lisa M. Broad, Gary St. J. Bird, and James W. Putney Jr.§

From the Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709

We have investigated the signaling pathways underlying muscarinic receptor-induced calcium oscillations in human embryonic kidney (HEK293) cells. Activation of muscarinic receptors with a maximal concentration of carbachol (100 µM) induced a biphasic rise in cytoplasmic calcium ([Ca2+]i) comprised of release of Ca2+ from intracellular stores and influx of Ca2+ from the extracellular space. A lower concentration of carbachol (5 µM) induced repetitive [Ca2+]i spikes or oscillations, the continuation of which was dependent on extracellular Ca2+. The entry of Ca2+ with 100 µM carbachol and with the sarcoplasmic-endoplasmic reticulum calcium ATPase inhibitor, thapsigargin, was completely blocked by 1 µM Gd3+, as well as 30-100 µM concentrations of the membrane-permeant inositol 1,4,5-trisphosphate receptor inhibitor, 2-aminoethyoxydiphenyl borane (2-APB). Sensitivity to these inhibitors is indicative of capacitative calcium entry. Arachidonic acid, a candidate signal for Ca2+ entry associated with [Ca2+]i oscillations in HEK293 cells, induced entry that was inhibited only by much higher concentrations of Gd3+ and was unaffected by 100 µM 2-APB. Like arachidonic acid-induced entry, the entry associated with [Ca2+]i oscillations was insensitive to inhibition by Gd3+ but was completely blocked by 100 µM 2-APB. These findings indicate that the signaling pathway responsible for the Ca2+ entry driving [Ca2+]i oscillations in HEK293 cells is more complex than originally thought, and may involve neither capacitative calcium entry nor a role for PLA2 and arachidonic acid.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Present address: Dept. of Pharmacology, Harbin Medical University, Harbin 150086, Peoples Republic of China.

§ To whom correspondence should be addressed: Laboratory of Signal Transduction, NIEHS, National Institutes of Health, P. O. Box 12233, Research Triangle Park, NC 27709. Tel.: 919-541-1420; Fax: 919-541-7879; E-mail: putney@niehs.nih.gov.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Pharmacol.Home page
A. C. L. Martin, D. Willoughby, A. Ciruela, L.-J. Ayling, M. Pagano, S. Wachten, A. Tengholm, and D. M. F. Cooper
Capacitative Ca2+ Entry via Orai1 and Stromal Interacting Molecule 1 (STIM1) Regulates Adenylyl Cyclase Type 8
Mol. Pharmacol., April 1, 2009; 75(4): 830 - 842.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. E. Peel, B. Liu, and I. P. Hall
ORAI and Store-Operated Calcium Influx in Human Airway Smooth Muscle Cells
Am. J. Respir. Cell Mol. Biol., June 1, 2008; 38(6): 744 - 749.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
D. M. Graham, K. Y. Wong, P. Shapiro, C. Frederick, K. Pattabiraman, and D. M. Berson
Melanopsin Ganglion Cells Use a Membrane-Associated Rhabdomeric Phototransduction Cascade
J Neurophysiol, May 1, 2008; 99(5): 2522 - 2532.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. K. Fellner and W. J. Arendshorst
Angiotensin II-stimulated Ca2+ entry mechanisms in afferent arterioles: role of transient receptor potential canonical channels and reverse Na+/Ca2+ exchange
Am J Physiol Renal Physiol, January 1, 2008; 294(1): F212 - F219.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Kaznacheyeva, L. Glushankova, V. Bugaj, O. Zimina, A. Skopin, V. Alexeenko, L. Tsiokas, I. Bezprozvanny, and G. N. Mozhayeva
Suppression of TRPC3 Leads to Disappearance of Store-operated Channels and Formation of a New Type of Store-independent Channels in A431 Cells
J. Biol. Chem., August 10, 2007; 282(32): 23655 - 23662.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol PatholHome page
J. K. Dunnick, G. Kissling, D. K. Gerken, M. A. Vallant, and A. Nyska
Cardiotoxicity of Ma Huang/Caffeine or Ephedrine/Caffeine in a Rodent Model System
Toxicol Pathol, August 1, 2007; 35(5): 657 - 664.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
D. Willoughby and D. M. F. Cooper
Organization and Ca2+ Regulation of Adenylyl Cyclases in cAMP Microdomains
Physiol Rev, July 1, 2007; 87(3): 965 - 1010.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
B. Wedel, R. R. Boyles, J. W. Putney Jr, and G. S. Bird
Role of the store-operated calcium entry proteins Stim1 and Orai1 in muscarinic cholinergic receptor-stimulated calcium oscillations in human embryonic kidney cells
J. Physiol., March 15, 2007; 579(3): 679 - 689.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
A. C. L. Martin and D. M. F. Cooper
Capacitative and 1-Oleyl-2-acetyl-sn-glycerol-Activated Ca2+ Entry Distinguished Using Adenylyl Cyclase Type 8
Mol. Pharmacol., August 1, 2006; 70(2): 769 - 777.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
B.-X. Zhang, X. Ma, W. Zhang, C.-K. Yeh, A. Lin, J. Luo, E. A. Sprague, R. H. Swerdlow, and M. S. Katz
Polyunsaturated fatty acids mobilize intracellular Ca2+ in NT2 human teratocarcinoma cells by causing release of Ca2+ from mitochondria
Am J Physiol Cell Physiol, May 1, 2006; 290(5): C1321 - C1333.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
T. Ebihara, F. Guo, L. Zhang, J. Y. Kim, and D. Saffen
Muscarinic Acetylcholine Receptors Stimulate Ca2+ Influx in PC12D Cells Predominantly via Activation of Ca2+ Store-Operated Channels.
J. Biochem., March 1, 2006; 139(3): 449 - 458.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
S. Kazerounian, G. M. Pitari, F. J. Shah, G. S. Frick, M. Madesh, I. Ruiz-Stewart, S. Schulz, G. Hajnoczky, and S. A. Waldman
Proliferative Signaling by Store-Operated Calcium Channels Opposes Colon Cancer Cell Cytostasis Induced by Bacterial Enterotoxins
J. Pharmacol. Exp. Ther., September 1, 2005; 314(3): 1013 - 1022.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
J.-P. Lievremont, G. S. Bird, and J. W. Putney Jr.
Mechanism of Inhibition of TRPC Cation Channels by 2-Aminoethoxydiphenylborane
Mol. Pharmacol., September 1, 2005; 68(3): 758 - 762.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. K. Zagranichnaya, X. Wu, and M. L. Villereal
Endogenous TRPC1, TRPC3, and TRPC7 Proteins Combine to Form Native Store-operated Channels in HEK-293 Cells
J. Biol. Chem., August 19, 2005; 280(33): 29559 - 29569.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
A. Y Estevez and K. Strange
Calcium feedback mechanisms regulate oscillatory activity of a TRP-like Ca2+ conductance in C. elegans intestinal cells
J. Physiol., August 15, 2005; 567(1): 239 - 251.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
K. Itagaki, K. B. Kannan, and C. J. Hauser
Lysophosphatidic acid triggers calcium entry through a non-store-operated pathway in human neutrophils
J. Leukoc. Biol., February 1, 2005; 77(2): 181 - 189.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
G. St. J Bird and J. W Putney Jr
Capacitative calcium entry supports calcium oscillations in human embryonic kidney cells
J. Physiol., February 1, 2005; 562(3): 697 - 706.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J.-P. Lievremont, G. St. J. Bird, and J. W. Putney Jr.
Canonical transient receptor potential TRPC7 can function as both a receptor- and store-operated channel in HEK-293 cells
Am J Physiol Cell Physiol, December 1, 2004; 287(6): C1709 - C1716.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
F. Zeng, S.-Z. Xu, P. K. Jackson, D. McHugh, B. Kumar, S. J. Fountain, and D. J. Beech
Human TRPC5 channel activated by a multiplicity of signals in a single cell
J. Physiol., September 15, 2004; 559(3): 739 - 750.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Shalabi, F. Zamudio, X. Wu, A. Scaloni, L. D. Possani, and M. L. Villereal
Tetrapandins, a New Class of Scorpion Toxins That Specifically Inhibit Store-operated Calcium Entry in Human Embryonic Kidney-293 Cells
J. Biol. Chem., January 9, 2004; 279(2): 1040 - 1049.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. H. Tarlowe, K. B. Kannan, K. Itagaki, J. M. Adams, D. H. Livingston, and C. J. Hauser
Inflammatory Chemoreceptor Cross-Talk Suppresses Leukotriene B4 Receptor 1-Mediated Neutrophil Calcium Mobilization and Chemotaxis After Trauma
J. Immunol., August 15, 2003; 171(4): 2066 - 2073.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
A. Y. Estevez, R. K. Roberts, and K. Strange
Identification of Store-independent and Store-operated Ca2+ Conductances in Caenorhabditis elegans Intestinal Epithelial Cells
J. Gen. Physiol., July 28, 2003; 122(2): 207 - 223.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Itagaki and C. J. Hauser
Sphingosine 1-Phosphate, a Diffusible Calcium Influx Factor Mediating Store-operated Calcium Entry
J. Biol. Chem., July 18, 2003; 278(30): 27540 - 27547.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
F.-J. Braun, O. Aziz, and J. W. Putney Jr.
2-Aminoethoxydiphenyl Borane Activates a Novel Calcium-Permeable Cation Channel
Mol. Pharmacol., June 1, 2003; 63(6): 1304 - 1311.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Trebak, G. St. J. Bird, R. R. McKay, L. Birnbaumer, and J. W. Putney Jr.
Signaling Mechanism for Receptor-activated Canonical Transient Receptor Potential 3 (TRPC3) Channels
J. Biol. Chem., April 25, 2003; 278(18): 16244 - 16252.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. De Luisi and A. M. Hofer
Evidence that Ca2+ cycling by the plasma membrane Ca2+-ATPase increases the `excitability' of the extracellular Ca2+-sensing receptor
J. Cell Sci., April 15, 2003; 116(8): 1527 - 1538.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
O. Mignen, J. L. Thompson, and T. J. Shuttleworth
Ca2+ Selectivity and Fatty Acid Specificity of the Noncapacitative, Arachidonate-regulated Ca2+ (ARC) Channels
J. Biol. Chem., March 14, 2003; 278(12): 10174 - 10181.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. H. Young, S. V. Wu, and E. Rozengurt
Ca2+-stimulated Ca2+ Oscillations Produced by the Ca2+-sensing Receptor Require Negative Feedback by Protein Kinase C
J. Biol. Chem., November 27, 2002; 277(49): 46871 - 46876.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Trebak, G. St. J. Bird, R. R. McKay, and J. W. Putney Jr.
Comparison of Human TRPC3 Channels in Receptor-activated and Store-operated Modes. DIFFERENTIAL SENSITIVITY TO CHANNEL BLOCKERS SUGGESTS FUNDAMENTAL DIFFERENCES IN CHANNEL COMPOSITION
J. Biol. Chem., June 7, 2002; 277(24): 21617 - 21623.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Xie, Y. Zhang, C. Zhai, and J. A. Bonanno
Calcium Influx Factor from Cytochrome P-450 Metabolism and Secretion-like Coupling Mechanisms for Capacitative Calcium Entry in Corneal Endothelial Cells
J. Biol. Chem., May 3, 2002; 277(19): 16559 - 16566.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Itagaki, K. B. Kannan, D. H. Livingston, E. A. Deitch, Z. Fekete, and C. J. Hauser
Store-Operated Calcium Entry in Human Neutrophils Reflects Multiple Contributions from Independently Regulated Pathways
J. Immunol., April 15, 2002; 168(8): 4063 - 4069.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Wu, G. Babnigg, T. Zagranichnaya, and M. L. Villereal
The Role of Endogenous Human Trp4 in Regulating Carbachol-induced Calcium Oscillations in HEK-293 Cells
J. Biol. Chem., April 12, 2002; 277(16): 13597 - 13608.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Interv.Home page
J. W. Putney Jr.
Pharmacology of Capacitative Calcium Entry
Mol. Interv., June 1, 2001; 1(2): 84 - 94.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Luo, L. M. Broad, G. St. J. Bird, and J. W. Putney Jr.
Mutual Antagonism of Calcium Entry by Capacitative and Arachidonic Acid-mediated Calcium Entry Pathways
J. Biol. Chem., June 1, 2001; 276(23): 20186 - 20189.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H.-T. Ma, K. Venkatachalam, H.-S. Li, C. Montell, T. Kurosaki, R. L. Patterson, and D. L. Gill
Assessment of the Role of the Inositol 1,4,5-Trisphosphate Receptor in the Activation of Transient Receptor Potential Channels and Store-operated Ca2+ Entry Channels
J. Biol. Chem., May 25, 2001; 276(22): 18888 - 18896.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
O. Mignen, J. L. Thompson, and T. J. Shuttleworth
Reciprocal Regulation of Capacitative and Arachidonate-regulated Noncapacitative Ca2+ Entry Pathways
J. Biol. Chem., September 14, 2001; 276(38): 35676 - 35683.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. H. Young and E. Rozengurt
Amino acids and Ca2+ stimulate different patterns of Ca2+ oscillations through the Ca2+-sensing receptor
Am J Physiol Cell Physiol, June 1, 2002; 282(6): C1414 - C1422.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement