JBC Origene Your Gene Company

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M007773200 on December 4, 2000

J. Biol. Chem., Vol. 276, Issue 8, 5753-5759, February 23, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/8/5753    most recent
M007773200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hanada, M.
Right arrow Articles by Tamura, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hanada, M.
Right arrow Articles by Tamura, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Regulation of the TAK1 Signaling Pathway by Protein Phosphatase 2C*

Masahito HanadaDagger §, Jun Ninomiya-Tsuji, Ken-ichiro KomakiDagger ||, Motoko OhnishiDagger , Koji KatsuraDagger , Ryunosuke Kanamaru§, Kunihiro Matsumoto, and Shinri TamuraDagger **

From the Departments of Dagger  Biochemistry and § Clinical Oncology, Institute of Development, Aging, and Cancer, Tohoku University, 4-1 Seiryomachi, Aoba-ku, Sendai 980-8575, the  Department of Molecular Biology, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, and the || Department of Geriatric Dentistry, Tohoku University School of Dentistry, 4-1 Seiryomachi, Aoba-ku, Sendai 980-8575, Japan

Protein phosphatase 2C (PP2C) is implicated in the negative regulation of stress-activated protein kinase cascades in yeast and mammalian cells. In this study, we determined the role of PP2Cbeta -1, a major isoform of mammalian PP2C, in the TAK1 signaling pathway, a stress-activated protein kinase cascade that is activated by interleukin-1, transforming growth factor-beta , or stress. Ectopic expression of PP2Cbeta -1 inhibited the TAK1-mediated mitogen-activated protein kinase kinase 4-c-Jun amino-terminal kinase and mitogen-activated protein kinase kinase 6-p38 signaling pathways. In vitro, PP2Cbeta -1 dephosphorylated and inactivated TAK1. Coimmunoprecipitation experiments indicated that PP2Cbeta -1 associates with the central region of TAK1. A phosphatase-negative mutant of PP2Cbeta -1, PP2Cbeta -1 (R/G), acted as a dominant negative mutant, inhibiting dephosphorylation of TAK1 by wild-type PP2Cbeta -1 in vitro. In addition, ectopic expression of PP2Cbeta -1(R/G) enhanced interleukin-1-induced activation of an AP-1 reporter gene. Collectively, these results indicate that PP2Cbeta negatively regulates the TAK1 signaling pathway by direct dephosphorylation of TAK1.

* This work was supported in part by a grant-in-aid for scientific research from the Ministry of Education, Science, Sports, and Culture of Japan and by the Smoking Research Foundation and the Takeda Science Foundation.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

** To whom correspondence should be addressed. Tel.: 81-22-717-8471; Fax: 81-22-717-8476; E-mail: tamura@idac.tohoku.ac.jp.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
P. M. Chan, L. Lim, and E. Manser
PAK Is Regulated by PI3K, PIX, CDC42, and PP2C{alpha} and Mediates Focal Adhesion Turnover in the Hyperosmotic Stress-induced p38 Pathway
J. Biol. Chem., September 5, 2008; 283(36): 24949 - 24961.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. D. Prickett, J. Ninomiya-Tsuji, P. Broglie, T. L. Muratore-Schroeder, J. Shabanowitz, D. F. Hunt, and D. L. Brautigan
TAB4 Stimulates TAK1-TAB1 Phosphorylation and Binds Polyubiquitin to Direct Signaling to NF-{kappa}B
J. Biol. Chem., July 11, 2008; 283(28): 19245 - 19254.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. I. Kim, J. H. Kwak, L. Wang, and M. E. Choi
Protein Phosphatase 2A Is a Negative Regulator of Transforming Growth Factor-{beta}1-induced TAK1 Activation in Mesangial Cells
J. Biol. Chem., April 18, 2008; 283(16): 10753 - 10763.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
M. R. Junttila, S.-P. Li, and J. Westermarck
Phosphatase-mediated crosstalk between MAPK signaling pathways in the regulation of cell survival
FASEB J, April 1, 2008; 22(4): 954 - 965.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
A. Doron-Faigenboim and T. Pupko
A Combined Empirical and Mechanistic Codon Model
Mol. Biol. Evol., February 1, 2007; 24(2): 388 - 397.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Kajino, H. Ren, S.-i. Iemura, T. Natsume, B. Stefansson, D. L. Brautigan, K. Matsumoto, and J. Ninomiya-Tsuji
Protein Phosphatase 6 Down-regulates TAK1 Kinase Activation in the IL-1 Signaling Pathway
J. Biol. Chem., December 29, 2006; 281(52): 39891 - 39896.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Qiao, S. R. Padilla, and P. D. Benya
Transforming Growth Factor (TGF)-{beta}-activated Kinase 1 Mimics and Mediates TGF-{beta}-induced Stimulation of Type II Collagen Synthesis in Chondrocytes Independent of Col2a1 Transcription and Smad3 Signaling
J. Biol. Chem., April 29, 2005; 280(17): 17562 - 17571.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Singhirunnusorn, S. Suzuki, N. Kawasaki, I. Saiki, and H. Sakurai
Critical Roles of Threonine 187 Phosphorylation in Cellular Stress-induced Rapid and Transient Activation of Transforming Growth Factor-{beta}-activated Kinase 1 (TAK1) in a Signaling Complex Containing TAK1-binding Protein TAB1 and TAB2
J. Biol. Chem., February 25, 2005; 280(8): 7359 - 7368.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
J. Kaput, K. G. Klein, E. J. Reyes, W. A. Kibbe, C. A. Cooney, B. Jovanovic, W. J. Visek, and G. L. Wolff
Identification of genes contributing to the obese yellow Avy phenotype: caloric restriction, genotype, diet x genotype interactions
Physiol Genomics, August 11, 2004; 18(3): 316 - 324.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. E. Rieger, W.-J. Hong, V. G. Tusher, J. Tang, R. Tibshirani, and G. Chu
Toxicity from radiation therapy associated with abnormal transcriptional responses to DNA damage
PNAS, April 27, 2004; 101(17): 6635 - 6640.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Prajapati, U. Verma, Y. Yamamoto, Y. T. Kwak, and R. B. Gaynor
Protein Phosphatase 2C{beta} Association with the I{kappa}B Kinase Complex Is Involved in Regulating NF-{kappa}B Activity
J. Biol. Chem., January 16, 2004; 279(3): 1739 - 1746.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Takezawa
Characterization of a Novel Plant PP2C-like Protein Ser/Thr Phosphatase as a Calmodulin-binding Protein
J. Biol. Chem., September 26, 2003; 278(39): 38076 - 38083.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
V. Delorme, X. Cayla, G. Faure, A. Garcia, and I. Tardieux
Actin Dynamics Is Controlled by a Casein Kinase II and Phosphatase 2C Interplay on Toxoplasma gondii Toxofilin
Mol. Biol. Cell, May 1, 2003; 14(5): 1900 - 1912.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Ofek, D. Ben-Meir, Z. Kariv-Inbal, M. Oren, and S. Lavi
Cell Cycle Regulation and p53 Activation by Protein Phosphatase 2Calpha
J. Biol. Chem., April 11, 2003; 278(16): 14299 - 14305.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. G. Li, K. Katsura, H. Nomiyama, K.-i. Komaki, J. Ninomiya-Tsuji, K. Matsumoto, T. Kobayashi, and S. Tamura
Regulation of the Interleukin-1-induced Signaling Pathways by a Novel Member of the Protein Phosphatase 2C Family (PP2Cepsilon )
J. Biol. Chem., March 28, 2003; 278(14): 12013 - 12021.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Mitsuhashi, H. Shima, N. Tanuma, N. Matsuura, M. Takekawa, T. Urano, T. Kataoka, M. Ubukata, and K. Kikuchi
Usage of Tautomycetin, a Novel Inhibitor of Protein Phosphatase 1 (PP1), Reveals That PP1 Is a Positive Regulator of Raf-1 in Vivo
J. Biol. Chem., January 3, 2003; 278(1): 82 - 88.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
C. Young, J. Mapes, J. Hanneman, S. Al-Zarban, and I. Ota
Role of Ptc2 Type 2C Ser/Thr Phosphatase in Yeast High-Osmolarity Glycerol Pathway Inactivation
Eukaryot. Cell, December 1, 2002; 1(6): 1032 - 1040.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.