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J. Biol. Chem., Vol. 276, Issue 8, 5866-5875, February 23, 2001
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From the Signal Transduction Laboratory, Institute of Molecular and
Cell Biology, National University of Singapore,
Singapore 117609, Singapore
Sprouty (SPRY) was first identified in a genetic
screen in Drosophila as an antagonist of fibroblast and
epidermal growth factor receptors and Sevenless signaling, seemingly by
inhibiting the receptor tyrosine kinase (RTK)/Ras/MAPK pathway. To
date, four mammalian Sprouty genes have been
identified; the primary sequences of the gene products share a well
conserved cysteine-rich C-terminal domain with their
Drosophila counterpart. The N-terminal regions do not,
however, exhibit a large degree of homology. This study was aimed at
identifying proteins with which human SPRY2 (hSPRY2) interacts in an
attempt to understand the mechanism by which Sprouty proteins exert
their down-regulatory effects. Here, we demonstrate that hSPRY2
associates directly with c-Cbl, a known down-regulator of RTK
signaling. A short sequence in the N terminus of hSPRY2 was found to
bind directly to the Ring finger domain of c-Cbl. Parallel binding was
apparent between the Drosophila homologs of Sprouty and
Cbl, with cross-species associations occurring at least in
vitro. Coexpression of hSPRY2 abrogated an increase in the rate
of epidermal growth factor receptor internalization induced by c-Cbl,
whereas a mutant hSPRY2 protein unable to bind c-Cbl showed no such
effect. Our results suggest that one function of hSPRY2 in signaling
processes downstream of RTKs may be to modulate c-Cbl physiological
function such as that seen with receptor-mediated endocytosis.
Evidence for Direct Interaction between Sprouty and Cbl*
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Signal Transduction
Lab., Inst. of Molecular and Cell Biology, National University of
Singapore, 30 Medical Dr., Singapore 117609. Tel.: 65-874-3794; Fax:
65-779-1117; E-mail: mcbgg@imcb.nus.edu.sg.
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