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J. Biol. Chem., Vol. 276, Issue 8, 5975-5984, February 23, 2001
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From the The Escherichia coli mazEF system is
a chromosomal "addiction module" that, under starvation conditions
in which guanosine-3',5'-bispyrophosphate (ppGpp) is produced, is
responsible for programmed cell death. This module specifies for the
toxic stable protein MazF and the labile antitoxic protein MazE.
Upstream from the mazEF module are two promoters,
P2 and P3 that are strongly negatively
autoregulated by MazE and MazF. We show that the expression of this
module is positively regulated by the factor for inversion
stimulation. What seems to be responsible for the negative
autoregulation of mazEF is an unusual DNA structure, which
we have called an "alternating palindrome." The middle part,
"a," of this structure may complement either the downstream
fragment, "b," or the upstream fragment, "c". When the
MazE·MazF complex binds either of these arms of the alternating
palindrome, strong negative autoregulation results. We suggest
that the combined presence of the two promoters, the alternating
palindrome structure and the factor for inversion stimulation-binding
site, all permit the expression of the mazEF module to be
sensitively regulated under various growth conditions.
The Regulation of the Escherichia coli mazEF Promoter
Involves an Unusual Alternating Palindrome*
,,¶
Department of Cellular Biochemistry and the
§ Department of Molecular Biology, Hebrew
University-Hadassah Medical School,
Ein Kerem, Jerusalem, 91120 Israel
*
This work was supported by Israel Science Foundation Grant
467/99-2 (to G. G.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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