| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 9, 6125-6132, March 2, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Botanisches Institut der Universität Bonn,
Karlrobert-Kreiten-Strasse 13, 53115 Bonn, Germany
Hydrogen evolution is observed in the green alga
Scenedesmus obliquus after a phase of anaerobic adaptation.
In this study we report the biochemical and genetical characterization
of a new type of iron hydrogenase (HydA) in this photosynthetic
organism. The monomeric enzyme has a molecular mass of 44.5 kDa. The
complete hydA cDNA of 2609 base pairs comprises
an open reading frame encoding a polypeptide of 448 amino acids. The
protein contains a short transit peptide that routes the nucleus
encoded hydrogenase to the chloroplast. Antibodies raised against the
iron hydrogenase from Chlamydomonas reinhardtii react with
both the isolated and in Escherichia coli overexpressed
protein of S. obliquus as shown by Western blotting. By
analyzing 5 kilobases of the genomic DNA, the transcription initiation
site and five introns within hydA were revealed. Northern
experiments suggest that hydA transcription is induced
during anaerobic incubation. Alignments of S. obliquus HydA
with known iron hydrogenases and sequencing of the N terminus of the
purified protein confirm that HydA belongs to the class of iron
hydrogenases. The C terminus of the enzyme including the catalytic site
(H cluster) reveals a high degree of identity to iron hydrogenases.
However, the lack of additional Fe-S clusters in the N-terminal domain
indicates a novel pathway of electron transfer. Inhibitor experiments
show that the ferredoxin PetF functions as natural electron donor
linking the enzyme to the photosynthetic electron transport chain. PetF
probably binds to the hydrogenase through electrostatic interactions.
This paper is dedicated to Jan Vlcek who found evidence for an iron
hydrogenase in S. obliquus for the first time.
Unfortunately, he died 4 years ago in a tragic car accident. The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AJ271546.
A Novel Type of Iron Hydrogenase in the Green Alga
Scenedesmus obliquus Is Linked to the Photosynthetic
Electron Transport Chain*
*
This work was supported by Deutsche Forschungsgemeinschaft
Grant Ha2555/1-1.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: E-mail:
t.happe@ uni-bonn.de.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Long, C. H. Chang, P. W. King, M. L. Ghirardi, and K. Kim Brownian Dynamics and Molecular Dynamics Study of the Association between Hydrogenase and Ferredoxin from Chlamydomonas reinhardtii Biophys. J., October 15, 2008; 95(8): 3753 - 3766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. Chang, P. W. King, M. L. Ghirardi, and K. Kim Atomic Resolution Modeling of the Ferredoxin:[FeFe] Hydrogenase Complex from Chlamydomonas reinhardtii Biophys. J., November 1, 2007; 93(9): 3034 - 3045. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. W. King, M. C. Posewitz, M. L. Ghirardi, and M. Seibert Functional Studies of [FeFe] Hydrogenase Maturation in an Escherichia coli Biosynthetic System J. Bacteriol., March 15, 2006; 188(6): 2163 - 2172. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Putz, P. Dolezal, G. Gelius-Dietrich, L. Bohacova, J. Tachezy, and K. Henze Fe-Hydrogenase Maturases in the Hydrogenosomes of Trichomonas vaginalis Eukaryot. Cell, March 1, 2006; 5(3): 579 - 586. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Kruse, J. Rupprecht, K.-P. Bader, S. Thomas-Hall, P. M. Schenk, G. Finazzi, and B. Hankamer Improved Photobiological H2 Production in Engineered Green Algal Cells J. Biol. Chem., October 7, 2005; 280(40): 34170 - 34177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Fouchard, A. Hemschemeier, A. Caruana, J. Pruvost, J. Legrand, T. Happe, G. Peltier, and L. Cournac Autotrophic and Mixotrophic Hydrogen Photoproduction in Sulfur-Deprived Chlamydomonas Cells Appl. Envir. Microbiol., October 1, 2005; 71(10): 6199 - 6205. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Cassan, B. Lagoutte, and P. Setif Ferredoxin-NADP+ Reductase: KINETICS OF ELECTRON TRANSFER, TRANSIENT INTERMEDIATES, AND CATALYTIC ACTIVITIES STUDIED BY FLASH-ABSORPTION SPECTROSCOPY WITH ISOLATED PHOTOSYSTEM I AND FERREDOXIN J. Biol. Chem., July 15, 2005; 280(28): 25960 - 25972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Girbal, G. von Abendroth, M. Winkler, P. M. C. Benton, I. Meynial-Salles, C. Croux, J. W. Peters, T. Happe, and P. Soucaille Homologous and Heterologous Overexpression in Clostridium acetobutylicum and Characterization of Purified Clostridial and Algal Fe-Only Hydrogenases with High Specific Activities Appl. Envir. Microbiol., May 1, 2005; 71(5): 2777 - 2781. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Tamagnini, R. Axelsson, P. Lindberg, F. Oxelfelt, R. Wunschiers, and P. Lindblad Hydrogenases and Hydrogen Metabolism of Cyanobacteria Microbiol. Mol. Biol. Rev., March 1, 2002; 66(1): 1 - 20. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-S. Yoon, C. Bobst, C. F. Hemann, R. Hille, and F. R. Tabita Spectroscopic and Functional Properties of Novel 2[4Fe-4S] Cluster-containing Ferredoxins from the Green Sulfur Bacterium Chlorobium tepidum J. Biol. Chem., November 16, 2001; 276(47): 44027 - 44036. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Melis and T. Happe Hydrogen Production. Green Algae as a Source of Energy Plant Physiology, November 1, 2001; 127(3): 740 - 748. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
