|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 9, 6267-6273, March 2, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Lipoxygenases are key enzymes in the
synthesis of oxylipins and play an important role in the response of
plants to wounding and pathogen attack. In cultured potato cells
treated with elicitor from Phytophthora infestans, the
causal agent of late blight disease, transcripts encoding a linoleate
9-lipoxygenase and a linoleate 13-lipoxygenase accumulate. However,
lipoxygenase activity assays and oxylipin profiling revealed only
increased 9-lipoxygenase activity and formation of products derived
therefrom, such as 9-hydroxy octadecadienoic acid and colneleic acid.
Furthermore, the 9-lipoxygenase products
9(S),10(S),11(R)-trihydroxy-12(Z)-octadecenoic and
9(S),10(S),11(R)-trihydroxy-12(Z),15(Z)-octadecadienoic
acid were identified as novel, elicitor-inducible oxylipins in potato, suggesting a role of these compounds in the defense response against pathogen attack. Neither 13-lipoxygenase activity nor 13-lipoxygenase products were detected in higher amounts in potato cells after elicitation. Thus, formation of products by the 9-lipoxygenase pathway,
including the enzymes hydroperoxide reductase, divinyl ether synthase,
and epoxy alcohol synthase, is preferentially stimulated in cultured
potato cells in response to treatment with P. infestans
elicitor. Moreover, elicitor-induced accumulation of desaturase
transcripts and increased phospholipase A2 activity after
elicitor treatment suggest that substrates for the lipoxygenase pathway
might be provided by de novo synthesis and subsequent release from lipids of the endomembrane system.
Oxylipin Profiling Reveals the Preferential
Stimulation of the 9-Lipoxygenase Pathway in Elicitor-treated
Potato Cells*
,
,,, and§§
Department of Stress and Developmental
Biology, ¶ Department of Secondary Metabolism, Institute of Plant
Biochemistry, Weinberg 3, Halle/Saale D-06120, Germany,
§ Department of Molecular Cell Biology, Institute of Plant
Genetics and Crop Plant Research, Corrensstrasse 3, Gatersleben
D-06466, Germany, ** Centro Nacional de Biotecnologia CSIC, Universidad
Autonoma de Madrid, Campus Cantoblanco, Madrid E-28049, Spain, and the
Department of Medical Biochemistry and
Biophysics, Division of Physiological Chemistry II, Karolinska
Institutet, Stockholm S-171 77, Sweden
*
This work was supported by the Fonds der Chemischen
Industrie.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Present address: Dept. of Molecular Biology,
Max-Planck-Institute of Chemical Ecology, Carl-Zeiss-Promenade 10, Jena
D-07745, Germany.
§§
To whom correspondence should be addressed: Tel.:
49-345-55821440; Fax: 49-345-55821409; E-mail:
srosahl@ipb-halle.de.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Kargiotidou, D. Deli, D. Galanopoulou, A. Tsaftaris, and T. Farmaki Low temperature and light regulate delta 12 fatty acid desaturases (FAD2) at a transcriptional level in cotton (Gossypium hirsutum) J. Exp. Bot., May 2, 2008; (2008) ern065v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. J. Mur, P. Kenton, A. J. Lloyd, H. Ougham, and E. Prats The hypersensitive response; the centenary is upon us but how much do we know? J. Exp. Bot., February 1, 2008; 59(3): 501 - 520. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Fammartino, F. Cardinale, C. Gobel, L. Mene-Saffrane, J. Fournier, I. Feussner, and M.-T. Esquerre-Tugaye Characterization of a Divinyl Ether Biosynthetic Pathway Specifically Associated with Pathogenesis in Tobacco Plant Physiology, January 1, 2007; 143(1): 378 - 388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. X. Andersson, M. Hamberg, O. Kourtchenko, A. Brunnstrom, K. L. McPhail, W. H. Gerwick, C. Gobel, I. Feussner, and M. Ellerstrom Oxylipin Profiling of the Hypersensitive Response in Arabidopsis thaliana: FORMATION OF A NOVEL OXO-PHYTODIENOIC ACID-CONTAINING GALACTOLIPID, ARABIDOPSIDE E J. Biol. Chem., October 20, 2006; 281(42): 31528 - 31537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Prost, S. Dhondt, G. Rothe, J. Vicente, M. J. Rodriguez, N. Kift, F. Carbonne, G. Griffiths, M.-T. Esquerre-Tugaye, S. Rosahl, et al. Evaluation of the Antimicrobial Activities of Plant Oxylipins Supports Their Involvement in Defense against Pathogens Plant Physiology, December 1, 2005; 139(4): 1902 - 1913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gobel, I. Feussner, and S. Rosahl Lipid Peroxidation during the Hypersensitive Response in Potato in the Absence of 9-Lipoxygenases J. Biol. Chem., December 26, 2003; 278(52): 52834 - 52840. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Itoh, A. L. Schilmiller, B. C. McCaig, and G. A. Howe Identification of a Jasmonate-regulated Allene Oxide Synthase That Metabolizes 9-Hydroperoxides of Linoleic and Linolenic Acids J. Biol. Chem., November 22, 2002; 277(48): 46051 - 46058. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Petters, C. Gobel, D. Scheel, and S. Rosahl A Pathogen-Responsive cDNA from Potato Encodes a Protein with Homology to a Phosphate Starvation-Induced Phosphatase Plant Cell Physiol., September 15, 2002; 43(9): 1049 - 1053. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Turner, C. Ellis, and A. Devoto The Jasmonate Signal Pathway PLANT CELL, May 1, 2002; 14(90001): S153 - 164. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |