Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M006537200 on November 30, 2000

J. Biol. Chem., Vol. 276, Issue 9, 6327-6336, March 2, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/9/6327    most recent
M006537200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lesourne, R.
Right arrow Articles by Daëron, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lesourne, R.
Right arrow Articles by Daëron, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Insufficient Phosphorylation Prevents Fcgamma RIIB from Recruiting the SH2 Domain-containing Protein-tyrosine Phosphatase SHP-1*

Renaud LesourneDagger , Pierre Bruhns, Wolf H. Fridman, and Marc Daëron§

From the Laboratoire d'Immunologie Cellulaire et Clinique, INSERM U.255, Institut Curie, 75005 Paris, France

Fcgamma RIIB are IgG receptors that inhibit immunoreceptor tyrosine-based activation motif (ITAM)-dependent cell activation. Inhibition depends on an immunoreceptor tyrosine-based inhibition motif (ITIM) that is phosphorylated upon Fcgamma RIIB coaggregation with ITAM-bearing receptors and recruits SH2 domain-containing phosphatases. Agarose bead-coated phosphorylated ITIM peptides (pITIMs) bind in vitro the single-SH2 inositol 5-phosphatases (SHIP1 and SHIP2) and the two-SH2 protein tyrosine phosphatases (SHP-1 and SHP-2). Phosphorylated Fcgamma RIIB, however, recruit selectively SHIP1/2 in vivo. We aimed here at explaining this discordance. We found that beads coated with low amounts of pITIM bound in vitro SHIP1, but not SHP-1, i.e. behaved as phosphorylated Fcgamma RIIB in vivo. The reason is that SHP-1 requires its two SH2 domains to bind on adjacent pITIMs. Consequently, the binding of SHP-1, but not of SHIP1, increased with pITIM density on beads. When trying to increase Fcgamma RIIB phosphorylation in B cells and mast cells, we found that concentrations of ligands optimal for Fcgamma RIIB phosphorylation failed to induce SHP-1 recruitment. SHP-1 was, however, recruited by Fcgamma RIIB when hyperphosphorylated following cell treatment with pervanadate. Our data suggest that Fcgamma RIIB phosphorylation may not be sufficient in vivo to enable the recruitment of SHP-1 but that (pathological?) conditions that would hyperphosphorylate Fcgamma RIIB might enable SHP-1 recruitment.

* This work was supported in part by the Institut National de la Santé et de la Recherche Médicale, the Institut Curie, and the Association pour la Recherche sur le Cancer.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Recipient of a fellowship from the Ministère de l'Enseignement Supérieur et de la Recherche.

§ To whom correspondence should be addressed: Laboratoire d'Immunologie Cellulaire et Clinique, INSERM U. 255, Institut Curie, 26 rue d'Ulm, 75005 Paris, France. Tel.: 33-1-4432-4223; Fax: 33-1-4051-0420; E-mail: Marc.Daeron@curie.fr.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
Y. Wang, R. J. Keogh, M. G. Hunter, C. A. Mitchell, R. S. Frey, K. Javaid, A. B. Malik, S. Schurmans, S. Tridandapani, and C. B. Marsh
SHIP2 Is Recruited to the Cell Membrane upon Macrophage Colony-Stimulating Factor (M-CSF) Stimulation and Regulates M-CSF-Induced Signaling
J. Immunol., December 1, 2004; 173(11): 6820 - 6830.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. L. Kepley, S. Taghavi, G. Mackay, D. Zhu, P. A. Morel, K. Zhang, J. J. Ryan, L. S. Satin, M. Zhang, P. P. Pandolfi, et al.
Co-aggregation of Fc{gamma}RII with Fc{epsilon}RI on Human Mast Cells Inhibits Antigen-induced Secretion and Involves SHIP-Grb2-Dok Complexes
J. Biol. Chem., August 20, 2004; 279(34): 35139 - 35149.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. R. Crow, S. Song, J. Freedman, C. D. Helgason, R. K. Humphries, K. A. Siminovitch, and A. H. Lazarus
IVIg-mediated amelioration of murine ITP via Fc{gamma}RIIB is independent of SHIP1, SHP-1, and Btk activity
Blood, July 15, 2003; 102(2): 558 - 560.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
Z.-Y. Huang, S. Hunter, M.-K. Kim, Z. K. Indik, and A. D. Schreiber
The effect of phosphatases SHP-1 and SHIP-1 on signaling by the ITIM- and ITAM-containing Fc{gamma} receptors Fc{gamma}RIIB and Fc{gamma}RIIA
J. Leukoc. Biol., June 1, 2003; 73(6): 823 - 829.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
E. Garcia-Garcia and C. Rosales
Signal transduction during Fc receptor-mediated phagocytosis
J. Leukoc. Biol., December 1, 2002; 72(6): 1092 - 1108.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Tridandapani, K. Siefker, J.-L. Teillaud, J. E. Carter, M. D. Wewers, and C. L. Anderson
Regulated Expression and Inhibitory Function of Fcgamma RIIb in Human Monocytic Cells
J. Biol. Chem., February 8, 2002; 277(7): 5082 - 5089.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
J. G. Marshall, J. W. Booth, V. Stambolic, T. Mak, T. Balla, A. D. Schreiber, T. Meyer, and S. Grinstein
Restricted Accumulation of Phosphatidylinositol 3-Kinase Products in a Plasmalemmal Subdomain during Fc{gamma} Receptor-mediated Phagocytosis
J. Cell Biol., June 18, 2001; 153(7): 1369 - 1380.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
O. Malbec, C. Schmitt, P. Bruhns, G. Krystal, W. H. Fridman, and M. Daeron
Src Homology 2 Domain-containing Inositol 5-Phosphatase 1 Mediates Cell Cycle Arrest by Fcgamma RIIB
J. Biol. Chem., August 3, 2001; 276(32): 30381 - 30391.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement