|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 9, 6770-6778, March 2, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Chitinases are ubiquitous chitin-fragmenting
hydrolases. Recently we discovered the first human chitinase, named
chitotriosidase, that is specifically expressed by phagocytes. We here
report the identification, purification, and subsequent cloning of a
second mammalian chitinase. This enzyme is characterized by an acidic isoelectric point and therefore named acidic mammalian chitinase (AMCase). In rodents and man the enzyme is relatively abundant in the
gastrointestinal tract and is found to a lesser extent in the lung.
Like chitotriosidase, AMCase is synthesized as a 50-kDa protein
containing a 39-kDa N-terminal catalytic domain, a hinge region, and a
C-terminal chitin-binding domain. In contrast to chitotriosidase, the
enzyme is extremely acid stable and shows a distinct second pH optimum
around pH 2. AMCase is capable of cleaving artificial chitin-like
substrates as well as crab shell chitin and chitin as present in the
fungal cell wall. Our study has revealed the existence of a
chitinolytic enzyme in the gastrointestinal tract and lung that may
play a role in digestion and/or defense.
Identification of a Novel Acidic Mammalian
Chitinase Distinct from Chitotriosidase*
§,,,,,
,, and
Department of Biochemistry, University of
Amsterdam, Academic Medical Center, 1105 AZ Amsterdam, The
Netherlands and the ¶ Center of Marine Biotechnology, University
of Maryland Biotechnology Institute, Baltimore, Maryland 21202
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Supported by National Science Foundation Grant IBN-9604265 and
by funds from the Center of Marine Biotechnology, University of
Maryland Biotechnology Institute.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. A. Seibold, T. A. Reese, S. Choudhry, M. T. Salam, K. Beckman, C. Eng, A. Atakilit, K. Meade, M. Lenoir, H. G. Watson, et al. Differential Enzymatic Activity of Common Haplotypic Versions of the Human Acidic Mammalian Chitinase Protein J. Biol. Chem., July 17, 2009; 284(29): 19650 - 19658. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. G. Lee, D. Hartl, G. R. Lee, B. Koller, H. Matsuura, C. A. Da Silva, M. H. Sohn, L. Cohn, R. J. Homer, A. A. Kozhich, et al. Role of breast regression protein 39 (BRP-39)/chitinase 3-like-1 in Th2 and IL-13-induced tissue responses and apoptosis J. Exp. Med., May 11, 2009; 206(5): 1149 - 1166. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Hartl, C. H. He, B. Koller, C. A. Da Silva, Y. Kobayashi, C. G. Lee, R. A. Flavell, and J. A. Elias Acidic Mammalian Chitinase Regulates Epithelial Cell Apoptosis via a Chitinolytic-Independent Mechanism J. Immunol., April 15, 2009; 182(8): 5098 - 5106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Da Silva, C. Chalouni, A. Williams, D. Hartl, C. G. Lee, and J. A. Elias Chitin Is a Size-Dependent Regulator of Macrophage TNF and IL-10 Production J. Immunol., March 15, 2009; 182(6): 3573 - 3582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Da Silva, D. Hartl, W. Liu, C. G. Lee, and J. A. Elias TLR-2 and IL-17A in Chitin-Induced Macrophage Activation and Acute Inflammation J. Immunol., September 15, 2008; 181(6): 4279 - 4286. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Flynn and G. Mulcahy Possible Role for Toll-Like Receptors in Interaction of Fasciola hepatica Excretory/Secretory Products with Bovine Macrophages Infect. Immun., February 1, 2008; 76(2): 678 - 684. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. L. Chupp, C. G. Lee, N. Jarjour, Y. M. Shim, C. T. Holm, S. He, J. D. Dziura, J. Reed, A. J. Coyle, P. Kiener, et al. A Chitinase-like Protein in the Lung and Circulation of Patients with Severe Asthma N. Engl. J. Med., November 15, 2007; 357(20): 2016 - 2027. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. P. Bussink, D. Speijer, J. M. F. G. Aerts, and R. G. Boot Evolution of Mammalian Chitinase(-Like) Members of Family 18 Glycosyl Hydrolases Genetics, October 1, 2007; 177(2): 959 - 970. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. DebRoy, J. Dao, M. Soderberg, O. Rossier, and N. P. Cianciotto Legionella pneumophila type II secretome reveals unique exoproteins and a chitinase that promotes bacterial persistence in the lung PNAS, December 12, 2006; 103(50): 19146 - 19151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Reece, M. C. Siracusa, and A. L. Scott Innate Immune Responses to Lung-Stage Helminth Infection Induce Alternatively Activated Alveolar Macrophages Infect. Immun., September 1, 2006; 74(9): 4970 - 4981. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Homer, Z. Zhu, L. Cohn, C. G. Lee, W. I. White, S. Chen, and J. A. Elias Differential expression of chitinases identify subsets of murine airway epithelial cells in allergic inflammation Am J Physiol Lung Cell Mol Physiol, September 1, 2006; 291(3): L502 - L511. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Nair, K. J. Guild, and D. Artis Novel Effector Molecules in Type 2 Inflammation: Lessons Drawn from Helminth Infection and Allergy J. Immunol., August 1, 2006; 177(3): 1393 - 1399. [Full Text] [PDF]
|
|
|
|
|
|
H. F. Bigg, R. Wait, A. D. Rowan, and T. E. Cawston The Mammalian Chitinase-like Lectin, YKL-40, Binds Specifically to Type I Collagen and Modulates the Rate of Type I Collagen Fibril Formation J. Biol. Chem., July 28, 2006; 281(30): 21082 - 21095. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Iwashita, S. Morita, Y. Sagiya, and A. Nakanishi Role of Eosinophil Chemotactic Factor by T Lymphocytes on Airway Hyperresponsiveness in a Murine Model of Allergic Asthma Am. J. Respir. Cell Mol. Biol., July 1, 2006; 35(1): 103 - 109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Kzhyshkowska, S. Mamidi, A. Gratchev, E. Kremmer, C. Schmuttermaier, L. Krusell, G. Haus, J. Utikal, K. Schledzewski, J. Scholtze, et al. Novel stabilin-1 interacting chitinase-like protein (SI-CLP) is up-regulated in alternatively activated macrophages and secreted via lysosomal pathway Blood, April 15, 2006; 107(8): 3221 - 3228. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Johansen, B. V. Jensen, A. Roslind, D. Nielsen, and P. A. Price Serum YKL-40, A New Prognostic Biomarker in Cancer Patients? Cancer Epidemiol. Biomarkers Prev., February 1, 2006; 15(2): 194 - 202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bierbaum, R. Nickel, A. Koch, S. Lau, K. A. Deichmann, U. Wahn, A. Superti-Furga, and A. Heinzmann Polymorphisms and Haplotypes of Acid Mammalian Chitinase Are Associated with Bronchial Asthma Am. J. Respir. Crit. Care Med., December 15, 2005; 172(12): 1505 - 1509. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. van Eijk, C. P. A. A. van Roomen, G. H. Renkema, A. P. Bussink, L. Andrews, E. F. C. Blommaart, A. Sugar, A. J. Verhoeven, R. G. Boot, and J. M. F. G. Aerts Characterization of human phagocyte-derived chitotriosidase, a component of innate immunity Int. Immunol., November 1, 2005; 17(11): 1505 - 1512. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Boot, A. P. Bussink, M. Verhoek, P. A.J. de Boer, A. F.M. Moorman, and J. M.F.G. Aerts Marked Differences in Tissue-specific Expression of Chitinases in Mouse and Man J. Histochem. Cytochem., October 1, 2005; 53(10): 1283 - 1292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. G. Boot, A. P. Bussink, and J. M. F. G. Aerts Human Acidic Mammalian Chitinase Erroneously Known as Eosinophil Chemotactic Cytokine Is Not the Ortholog of Mouse Ym1 J. Immunol., August 15, 2005; 175(4): 2041 - 2042. [Full Text] [PDF]
|
|
|
|
|
|
M. B. Joshi, M. E. Rogers, A. M. Shakarian, M. Yamage, S. A. Al-Harthi, P. A. Bates, and D. M. Dwyer Molecular Characterization, Expression, and in Vivo Analysis of LmexCht1: THE CHITINASE OF THE HUMAN PATHOGEN, LEISHMANIA MEXICANA J. Biol. Chem., February 4, 2005; 280(5): 3847 - 3861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Nair, I. J. Gallagher, M. D. Taylor, P. Loke, P. S. Coulson, R. A. Wilson, R. M. Maizels, and J. E. Allen Chitinase and Fizz Family Members Are a Generalized Feature of Nematode Infection with Selective Upregulation of Ym1 and Fizz1 by Antigen-Presenting Cells Infect. Immun., January 1, 2005; 73(1): 385 - 394. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Boer, N. Munck, J. Natunen, G. Wohlfahrt, H. Soderlund, O. Renkonen, and A. Koivula Differential recognition of animal type {beta}4-galactosylated and {alpha}3-fucosylated chito-oligosaccharides by two family 18 chitinases from Trichoderma harzianum Glycobiology, December 1, 2004; 14(12): 1303 - 1313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Wills-Karp and C. L. Karp Chitin Checking -- Novel Insights into Asthma N. Engl. J. Med., September 30, 2004; 351(14): 1455 - 1457. [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Zhu, T. Zheng, R. J. Homer, Y.-K. Kim, N. Y. Chen, L. Cohn, Q. Hamid, and J. A. Elias Acidic Mammalian Chitinase in Asthmatic Th2 Inflammation and IL-13 Pathway Activation Science, June 11, 2004; 304(5677): 1678 - 1682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Zimmermann, A. Mishra, N. E. King, P. C. Fulkerson, M. P. Doepker, N. M. Nikolaidis, L. E. Kindinger, E. A. Moulton, B. J. Aronow, and M. E. Rothenberg Transcript Signatures in Experimental Asthma: Identification of STAT6-Dependent and -Independent Pathways J. Immunol., February 1, 2004; 172(3): 1815 - 1824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Aguilera, K. Ghauharali-van der Vlugt, M. T. J. Helmond, J. M. M. Out, W. E. Donker-Koopman, J. E. M. Groener, R. G. Boot, G. H. Renkema, G. A. van der Marel, J. H. van Boom, et al. Transglycosidase Activity of Chitotriosidase: IMPROVED ENZYMATIC ASSAY FOR THE HUMAN MACROPHAGE CHITINASE J. Biol. Chem., October 17, 2003; 278(42): 40911 - 40916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. G. Sandler, M. M. Mentink-Kane, A. W. Cheever, and T. A. Wynn Global Gene Expression Profiles During Acute Pathogen-Induced Pulmonary Inflammation Reveal Divergent Roles for Th1 and Th2 Responses in Tissue Repair J. Immunol., October 1, 2003; 171(7): 3655 - 3667. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Artieda, A. Cenarro, A. Ganan, I. Jerico, C. Gonzalvo, J. M. Casado, I. Vitoria, J. Puzo, M. Pocovi, and F. Civeira Serum Chitotriosidase Activity Is Increased in Subjects With Atherosclerosis Disease Arterioscler. Thromb. Vasc. Biol., September 1, 2003; 23(9): 1645 - 1652. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. V. Rao, D. R. Houston, R. G. Boot, J. M. F. G. Aerts, S. Sakuda, and D. M. F. Van Aalten Crystal Structures of Allosamidin Derivatives in Complex with Human Macrophage Chitinase J. Biol. Chem., May 23, 2003; 278(22): 20110 - 20116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xu, J. C. Clark, B. J. Aronow, C. R. Dey, C. Liu, J. L. Wooldridge, and J. A. Whitsett Transcriptional Adaptation to Cystic Fibrosis Transmembrane Conductance Regulator Deficiency J. Biol. Chem., February 21, 2003; 278(9): 7674 - 7682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Suzuki, W. Fujimoto, M. Goto, M. Morimatsu, B. Syuto, and T. Iwanaga Cellular Expression of Gut Chitinase mRNA in the Gastrointestinal Tract of Mice and Chickens J. Histochem. Cytochem., August 1, 2002; 50(8): 1081 - 1089. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Houston, K. Shiomi, N. Arai, S. Omura, M. G. Peter, A. Turberg, B. Synstad, V. G. H. Eijsink, and D. M. F. van Aalten High-resolution structures of a chitinase complexed with natural product cyclopentapeptide inhibitors: Mimicry of carbohydrate substrate PNAS, July 9, 2002; 99(14): 9127 - 9132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Fusetti, H. von Moeller, D. Houston, H. J. Rozeboom, B. W. Dijkstra, R. G. Boot, J. M. F. G. Aerts, and D. M. F. van Aalten Structure of Human Chitotriosidase. IMPLICATIONS FOR SPECIFIC INHIBITOR DESIGN AND FUNCTION OF MAMMALIAN CHITINASE-LIKE LECTINS J. Biol. Chem., July 5, 2002; 277(28): 25537 - 25544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Harbord, M. Novelli, B. Canas, D. Power, C. Davis, J. Godovac-Zimmermann, J. Roes, and A. W. Segal Ym1 Is a Neutrophil Granule Protein That Crystallizes in p47phox-deficient Mice J. Biol. Chem., February 8, 2002; 277(7): 5468 - 5475. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |