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J. Biol. Chem., Vol. 276, Issue 9, 6867-6873, March 2, 2001
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From the Department of Biochemistry, Fukuoka University School of
Medicine, Jonan-ku, Fukuoka 814-0180, Japan and the
Giantin is a resident Golgi protein that has an
extremely long cytoplasmic domain (about 370 kDa) and is anchored to
the Golgi membrane by the COOH-terminal membrane-anchoring
domain (CMD) with no luminal extension. We examined the essential
domain of giantin required for Golgi localization by mutational
analysis. The Golgi localization of giantin was not affected by the
deletion of its CMD or by substitution with the CMD of syntaxin-2, a
plasma membrane protein. The giantin CMD fused to the cytoplasmic
domain of syntaxin-2 could not retain the chimera in the Golgi
apparatus. Sequential deletion analysis showed that the COOH-terminal
sequence (positions 3059-3161) adjacent to the CMD was the essential
domain required for the Golgi localization of giantin. We also examined two other Golgi-resident proteins, golgin-84 and syntaxin-5, with a
similar membrane topology as giantin. It was confirmed that the
cytoplasmic domain of about 100 residues adjacent to the CMD was
required for their Golgi localization. Taken together, these results
suggest that the COOH-terminally anchored Golgi proteins with long
cytoplasmic extensions have the Golgi localization signal(s) in the
cytoplasmic sequence adjacent to the CMD. This is in contrast to
previous observations that a transmembrane domain is required for Golgi
localization by other Golgi proteins transported from the endoplasmic reticulum.
An Essential Cytoplasmic Domain for the Golgi Localization of
Coiled-coil Proteins with a COOH-terminal Membrane Anchor*
, and Department of Virology and Molecular Oncology, Cancer
Research Institute, Kanazawa University, Kanazawa 920-0934, Japan
*
This work was supported in part by grants from the Ministry
of Education, Science, Sports, and Culture of Japan, the Japan, Science
and Technology Corporation (CREST), and the Central Research Institute
of Fukuoka University.The costs of publication of this article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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