JBC Advanced Peptides, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M109734200 on October 23, 2001

J. Biol. Chem., Vol. 277, Issue 1, 194-200, January 4, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
277/1/194    most recent
M109734200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Peterbauer, T.
Right arrow Articles by Richter, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Peterbauer, T.
Right arrow Articles by Richter, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Chain Elongation of Raffinose in Pea Seeds
ISOLATION, CHARCTERIZATION, AND MOLECULAR CLONING OF A MULTIFUNCTIONAL ENZYME CATALYZING THE SYNTHESIS OF STACHYOSE AND VERBASCOSE*,

Thomas PeterbauerDagger , Jan Mucha§, Lukas Mach§, and Andreas RichterDagger

From the Dagger  Institute of Ecology, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria and the § Centre for Applied Genetics, University of Agricultural Sciences Vienna, Muthgasse 18, 1190 Vienna, Austria

Raffinose oligosaccharides are major soluble carbohydrates in seeds and other tissues of plants. Their biosynthesis proceeds by stepwise addition of galactose units to sucrose, which are provided by the unusual donor galactinol (O-alpha -D-galactopyranosyl-(1right-arrow1)-L-myo-inositol). Chain elongation may also proceed by transfer of galactose units between raffinose oligosaccharides. We here report on the purification, characterization, and heterologous expression of a multifunctional stachyose synthase (EC 2.4.1.67) from developing pea (Pisum sativum L.) seeds. The protein, a member of family 36 of glycoside hydrolases, catalyzes the synthesis of stachyose, the tetrasaccharide of the raffinose series, by galactosyl transfer from galactinol to raffinose. It also mediates the synthesis of the pentasaccharide verbascose by galactosyl transfer from galactinol to stachyose as well as by self-transfer of the terminal galactose residue from one stachyose molecule to another. These activities show optima at pH 7.0. The enzyme also catalyzes hydrolysis of the terminal galactose residue of its substrates, but is unable to initiate the synthesis of raffinose oligosaccharides by galactosyl transfer from galactinol to sucrose. A minimum reaction mechanism which accounts for the broad substrate specificity and the steady-state kinetic properties of the protein is presented.

* This work was supported by Austrian Science Fund (FWF) Grant P13955-BIO (to A. R.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains Equations S1---S6 and Table SI.

The nucleotide sequence(s) reported in this paper for pea stachyose synthase has been deposited in the GenBankTM/EBI Data Bank with accession number(s) AJ311087.

To whom correspondence should be addressed: Chemical Physiology of Plants, Institute of Ecology, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria. Tel.: 43-1-4277-54252; Fax: 43-1-4277-9542; E-mail: Andreas.Richter@univie.ac.at.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
S. Peters, S. G. Mundree, J. A. Thomson, J. M. Farrant, and F. Keller
Protection mechanisms in the resurrection plant Xerophyta viscosa (Baker): both sucrose and raffinose family oligosaccharides (RFOs) accumulate in leaves in response to water deficit
J. Exp. Bot., June 1, 2007; 58(8): 1947 - 1956.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
U. Karner, T. Peterbauer, V. Raboy, David. A. Jones, C. L. Hedley, and A. Richter
myo-Inositol and sucrose concentrations affect the accumulation of raffinose family oligosaccharides in seeds
J. Exp. Bot., September 1, 2004; 55(405): 1981 - 1987.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. M. Tapernoux-Luthi, A. Bohm, and F. Keller
Cloning, Functional Expression, and Characterization of the Raffinose Oligosaccharide Chain Elongation Enzyme, Galactan:Galactan Galactosyltransferase, from Common Bugle Leaves
Plant Physiology, April 1, 2004; 134(4): 1377 - 1387.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
A. D. Elbein, Y.T. Pan, I. Pastuszak, and D. Carroll
New insights on trehalose: a multifunctional molecule
Glycobiology, April 1, 2003; 13(4): 17R - 27R.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.