HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 277, Issue 1, 609-617, January 4, 2002

This Article Full Text Full Text (PDF) All Versions of this Article: 277/1/609    most recent M108861200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hao, M. Articles by Maxfield, F. R. Search for Related Content PubMed PubMed Citation Articles by Hao, M. Articles by Maxfield, F. R. Social Bookmarking                      What's this?

Vesicular and Non-vesicular Sterol Transport in Living Cells
THE ENDOCYTIC RECYCLING COMPARTMENT IS A MAJOR STEROL STORAGE ORGANELLE^*

Mingming Hao^§, Sharron X. Lin, Ola J. Karylowski, Daniel Wüstner, Timothy E. McGraw, and Frederick R. Maxfield^¶

From the  Department of Biochemistry, Weill Medical College of Cornell University, New York, New York 10021 and the ^§ Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853

We examined the intracellular transport of sterol in living cells using a naturally fluorescent cholesterol analog, dehydroergosterol (DHE), which has been shown to mimic many of the properties of cholesterol. By using DHE loaded on methyl--cyclodextrin, we followed this cholesterol analog in pulse-chase studies. At steady state, DHE co-localizes extensively with transferrin (Tf), a marker for the endocytic recycling compartment (ERC), and redistributes with Tf in cells with altered ERC morphology. Expression of a dominant-negative mutation of an ERC-associated protein, mRme-1 (G429R), results in the slowing of both DHE and Tf receptor return to the cell surface. [³H]Cholesterol is found in the same fraction as ¹²⁵I-Tf on sucrose density gradients, and this fraction can be specifically shifted to a higher density based on the presence of horseradish peroxidase-conjugated Tf in the same organelle. Whereas vesicular transport of Tf and efflux of DHE from the ERC are entirely blocked in energy-depleted cells, delivery of DHE to the ERC from the plasma membrane is only slightly affected. Biochemical studies performed using [³H]cholesterol show that the energy dependence of cholesterol transport to and from the ERC is similar to DHE transport. We propose that a large portion of intracellular cholesterol is localized in the ERC, and this pool might be important in maintaining cellular cholesterol homeostasis.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				Y. Yamauchi, P. C. Reid, J. B. Sperry, K. Furukawa, M. Takeya, C. C. Y. Chang, and T.-Y. Chang Plasma Membrane Rafts Complete Cholesterol Synthesis by Participating in Retrograde Movement of Precursor Sterols J. Biol. Chem., November 30, 2007; 282(48): 34994 - 35004. [Abstract] [Full Text] [PDF]

				M. Hao, W. S. Head, S. C. Gunawardana, A. H. Hasty, and D. W. Piston Direct Effect of Cholesterol on Insulin Secretion: A Novel Mechanism for Pancreatic {beta}-Cell Dysfunction Diabetes, September 1, 2007; 56(9): 2328 - 2338. [Abstract] [Full Text] [PDF]

				M. Takahashi, M. Murate, M. Fukuda, S. B. Sato, A. Ohta, and T. Kobayashi Cholesterol Controls Lipid Endocytosis through Rab11 Mol. Biol. Cell, July 1, 2007; 18(7): 2667 - 2677. [Abstract] [Full Text] [PDF]

				J. Leyt, N. Melamed-Book, J.-P. Vaerman, S. Cohen, A. M. Weiss, and B. Aroeti Cholesterol-sensitive Modulation of Transcytosis Mol. Biol. Cell, June 1, 2007; 18(6): 2057 - 2071. [Abstract] [Full Text] [PDF]

				M. D. Linder, R.-L. Uronen, M. Holtta-Vuori, P. van der Sluijs, J. Peranen, and E. Ikonen Rab8-dependent Recycling Promotes Endosomal Cholesterol Removal in Normal and Sphingolipidosis Cells Mol. Biol. Cell, January 1, 2007; 18(1): 47 - 56. [Abstract] [Full Text] [PDF]

				D. Wustner Plasma Membrane Sterol Distribution Resembles the Surface Topography of Living Cells Mol. Biol. Cell, January 1, 2007; 18(1): 211 - 228. [Abstract] [Full Text] [PDF]

				S. Sugii, S. Lin, N. Ohgami, M. Ohashi, C. C. Y. Chang, and T.-Y. Chang Roles of Endogenously Synthesized Sterols in the Endocytic Pathway J. Biol. Chem., August 11, 2006; 281(32): 23191 - 23206. [Abstract] [Full Text] [PDF]

				D. Rodriguez-Agudo, S. Ren, P. B. Hylemon, R. Montanez, K. Redford, R. Natarajan, M. A. Medina, G. Gil, and W. M. Pandak Localization of StarD5 cholesterol binding protein J. Lipid Res., June 1, 2006; 47(6): 1168 - 1175. [Abstract] [Full Text] [PDF]

				N. A. Braun, P. J. Mohler, K. H. Weisgraber, A. H. Hasty, M. F. Linton, P. G. Yancey, Y. R. Su, S. Fazio, and L. L. Swift Intracellular trafficking of recycling apolipoprotein E in Chinese hamster ovary cells J. Lipid Res., June 1, 2006; 47(6): 1176 - 1186. [Abstract] [Full Text] [PDF]

				L. Yu, S. Bharadwaj, J. M. Brown, Y. Ma, W. Du, M. A. Davis, P. Michaely, P. Liu, M. C. Willingham, and L. L. Rudel Cholesterol-regulated Translocation of NPC1L1 to the Cell Surface Facilitates Free Cholesterol Uptake J. Biol. Chem., March 10, 2006; 281(10): 6616 - 6624. [Abstract] [Full Text] [PDF]

				N. H. Pipalia, A. Huang, H. Ralph, M. Rujoi, and F. R. Maxfield Automated microscopy screening for compounds that partially revert cholesterol accumulation in Niemann-Pick C cells J. Lipid Res., February 1, 2006; 47(2): 284 - 301. [Abstract] [Full Text] [PDF]

				N. M. Urs, K. T. Jones, P. D. Salo, J. E. Severin, J. Trejo, and H. Radhakrishna A requirement for membrane cholesterol in the {beta}-arrestin- and clathrin-dependent endocytosis of LPA1 lysophosphatidic acid receptors J. Cell Sci., November 15, 2005; 118(22): 5291 - 5304. [Abstract] [Full Text] [PDF]

				M. A. Churchward, T. Rogasevskaia, J. Hofgen, J. Bau, and J. R. Coorssen Cholesterol facilitates the native mechanism of Ca2+-triggered membrane fusion J. Cell Sci., October 15, 2005; 118(20): 4833 - 4848. [Abstract] [Full Text] [PDF]

				R. S. Kiss, J. Maric, and Y. L. Marcel Lipid efflux in human and mouse macrophagic cells: evidence for differential regulation of phospholipid and cholesterol efflux J. Lipid Res., September 1, 2005; 46(9): 1877 - 1887. [Abstract] [Full Text] [PDF]

				M. Holtta-Vuori, F. Alpy, K. Tanhuanpaa, E. Jokitalo, A.-L. Mutka, and E. Ikonen MLN64 Is Involved in Actin-mediated Dynamics of Late Endocytic Organelles Mol. Biol. Cell, August 1, 2005; 16(8): 3873 - 3886. [Abstract] [Full Text] [PDF]

				E. S. Ward, C. Martinez, C. Vaccaro, J. Zhou, Q. Tang, and R. J. Ober From Sorting Endosomes to Exocytosis: Association of Rab4 and Rab11 GTPases with the Fc Receptor, FcRn, during Recycling Mol. Biol. Cell, April 1, 2005; 16(4): 2028 - 2038. [Abstract] [Full Text] [PDF]

				R. Ishitsuka, S. B. Sato, and T. Kobayashi Imaging Lipid Rafts J. Biochem., March 1, 2005; 137(3): 249 - 254. [Abstract] [Full Text] [PDF]

				D. S. Ory Nuclear Receptor Signaling in the Control of Cholesterol Homeostasis: Have the Orphans Found a Home? Circ. Res., October 1, 2004; 95(7): 660 - 670. [Abstract] [Full Text] [PDF]

				G. Vassiliou and R. McPherson Role of cholesteryl ester transfer protein in selective uptake of high density lipoprotein cholesteryl esters by adipocytes J. Lipid Res., September 1, 2004; 45(9): 1683 - 1693. [Abstract] [Full Text] [PDF]

				G. Vassiliou and R. McPherson A Novel Efflux-Recapture Process Underlies the Mechanism of High-Density Lipoprotein Cholesteryl Ester-Selective Uptake Mediated by the Low-Density Lipoprotein Receptor-Related Protein Arterioscler. Thromb. Vasc. Biol., September 1, 2004; 24(9): 1669 - 1675. [Abstract] [Full Text] [PDF]

				N. Naslavsky, R. Weigert, and J. G. Donaldson Characterization of a Nonclathrin Endocytic Pathway: Membrane Cargo and Lipid Requirements Mol. Biol. Cell, August 1, 2004; 15(8): 3542 - 3552. [Abstract] [Full Text] [PDF]

				D. Rhainds, P. Bourgeois, G. Bourret, K. Huard, L. Falstrault, and L. Brissette Localization and regulation of SR-BI in membrane rafts of HepG2 cells J. Cell Sci., July 1, 2004; 117(15): 3095 - 3105. [Abstract] [Full Text] [PDF]

				R. E. Soccio and J. L. Breslow Intracellular Cholesterol Transport Arterioscler. Thromb. Vasc. Biol., July 1, 2004; 24(7): 1150 - 1160. [Abstract] [Full Text] [PDF]

				N. Wang, D. Lan, W. Chen, F. Matsuura, and A. R. Tall ATP-binding cassette transporters G1 and G4 mediate cellular cholesterol efflux to high-density lipoproteins PNAS, June 29, 2004; 101(26): 9774 - 9779. [Abstract] [Full Text] [PDF]

				O. W. Lindwasser and M. D. Resh Human Immunodeficiency Virus Type 1 Gag Contains a Dileucine-Like Motif That Regulates Association with Multivesicular Bodies J. Virol., June 1, 2004; 78(11): 6013 - 6023. [Abstract] [Full Text] [PDF]

				S. B. Sato, K. Ishii, A. Makino, K. Iwabuchi, A. Yamaji-Hasegawa, Y. Senoh, I. Nagaoka, H. Sakuraba, and T. Kobayashi Distribution and Transport of Cholesterol-rich Membrane Domains Monitored by a Membrane-impermeant Fluorescent Polyethylene Glycol-derivatized Cholesterol J. Biol. Chem., May 28, 2004; 279(22): 23790 - 23796. [Abstract] [Full Text] [PDF]

				N. Naslavsky, M. Boehm, P. S. Backlund Jr., and S. Caplan Rabenosyn-5 and EHD1 Interact and Sequentially Regulate Protein Recycling to the Plasma Membrane Mol. Biol. Cell, May 1, 2004; 15(5): 2410 - 2422. [Abstract] [Full Text] [PDF]

				M. Hao, S. Mukherjee, Y. Sun, and F. R. Maxfield Effects of Cholesterol Depletion and Increased Lipid Unsaturation on the Properties of Endocytic Membranes J. Biol. Chem., April 2, 2004; 279(14): 14171 - 14178. [Abstract] [Full Text] [PDF]

				D. Wustner, M. Mondal, A. Huang, and F. R. Maxfield Different transport routes for high density lipoprotein and its associated free sterol in polarized hepatic cells J. Lipid Res., March 1, 2004; 45(3): 427 - 437. [Abstract] [Full Text] [PDF]

				I. Coppens and K. A. Joiner Host but Not Parasite Cholesterol Controls Toxoplasma Cell Entry by Modulating Organelle Discharge Mol. Biol. Cell, September 1, 2003; 14(9): 3804 - 3820. [Abstract] [Full Text] [PDF]

				P. L. TUMA and A. L. HUBBARD Transcytosis: Crossing Cellular Barriers Physiol Rev, July 1, 2003; 83(3): 871 - 932. [Abstract] [Full Text] [PDF]

				S. Sugii, P. C. Reid, N. Ohgami, Y. Shimada, R. A. Maue, H. Ninomiya, Y. Ohno-Iwashita, and T.-Y. Chang Biotinylated {theta}-toxin derivative as a probe to examine intracellular cholesterol-rich domains in normal and Niemann-Pick type C1 cells J. Lipid Res., May 1, 2003; 44(5): 1033 - 1041. [Abstract] [Full Text] [PDF]

				K. M. Wojtanik and L. Liscum The Transport of Low Density Lipoprotein-derived Cholesterol to the Plasma Membrane Is Defective in NPC1 Cells J. Biol. Chem., April 18, 2003; 278(17): 14850 - 14856. [Abstract] [Full Text] [PDF]

				V. Wachtler, S. Rajagopalan, and M. K. Balasubramanian Sterol-rich plasma membrane domains in the fission yeast Schizosaccharomyces pombe J. Cell Sci., March 1, 2003; 116(5): 867 - 874. [Abstract] [Full Text] [PDF]

				T. L. Steck, J. Ye, and Y. Lange Probing Red Cell Membrane Cholesterol Movement with Cyclodextrin Biophys. J., October 1, 2002; 83(4): 2118 - 2125. [Abstract] [Full Text] [PDF]

				D. Wustner, A. Herrmann, M. Hao, and F. R. Maxfield Rapid Nonvesicular Transport of Sterol between the Plasma Membrane Domains of Polarized Hepatic Cells J. Biol. Chem., August 9, 2002; 277(33): 30325 - 30336. [Abstract] [Full Text] [PDF]

				R. E. Soccio, R. M. Adams, M. J. Romanowski, E. Sehayek, S. K. Burley, and J. L. Breslow The cholesterol-regulated StarD4 gene encodes a StAR-related lipid transfer protein with two closely related homologues, StarD5 and StarD6 PNAS, May 14, 2002; 99(10): 6943 - 6948. [Abstract] [Full Text] [PDF]