Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M108861200 on October 26, 2001

J. Biol. Chem., Vol. 277, Issue 1, 609-617, January 4, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/1/609    most recent
M108861200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hao, M.
Right arrow Articles by Maxfield, F. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hao, M.
Right arrow Articles by Maxfield, F. R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Vesicular and Non-vesicular Sterol Transport in Living Cells
THE ENDOCYTIC RECYCLING COMPARTMENT IS A MAJOR STEROL STORAGE ORGANELLE*

Mingming HaoDagger §, Sharron X. LinDagger , Ola J. KarylowskiDagger , Daniel WüstnerDagger , Timothy E. McGrawDagger , and Frederick R. MaxfieldDagger

From the Dagger  Department of Biochemistry, Weill Medical College of Cornell University, New York, New York 10021 and the § Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853

We examined the intracellular transport of sterol in living cells using a naturally fluorescent cholesterol analog, dehydroergosterol (DHE), which has been shown to mimic many of the properties of cholesterol. By using DHE loaded on methyl-beta -cyclodextrin, we followed this cholesterol analog in pulse-chase studies. At steady state, DHE co-localizes extensively with transferrin (Tf), a marker for the endocytic recycling compartment (ERC), and redistributes with Tf in cells with altered ERC morphology. Expression of a dominant-negative mutation of an ERC-associated protein, mRme-1 (G429R), results in the slowing of both DHE and Tf receptor return to the cell surface. [3H]Cholesterol is found in the same fraction as 125I-Tf on sucrose density gradients, and this fraction can be specifically shifted to a higher density based on the presence of horseradish peroxidase-conjugated Tf in the same organelle. Whereas vesicular transport of Tf and efflux of DHE from the ERC are entirely blocked in energy-depleted cells, delivery of DHE to the ERC from the plasma membrane is only slightly affected. Biochemical studies performed using [3H]cholesterol show that the energy dependence of cholesterol transport to and from the ERC is similar to DHE transport. We propose that a large portion of intracellular cholesterol is localized in the ERC, and this pool might be important in maintaining cellular cholesterol homeostasis.

* This work was supported by National Institutes of Health Grants DK27083 (to F. R. M.) and DK57689 (to T. E. M.) and a grant from the Ara Parseghian Medical Research Foundation.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Dept. of Biochemistry, Weill Medical College of Cornell University, 1300 York Ave., New York, NY 10021. Tel.: 212-746-6405; Fax: 212-746-8875; E-mail: frmaxfie@med.cornell.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
M. Mondal, B. Mesmin, S. Mukherjee, and F. R. Maxfield
Sterols Are Mainly in the Cytoplasmic Leaflet of the Plasma Membrane and the Endocytic Recycling Compartment in CHO Cells
Mol. Biol. Cell, January 1, 2009; 20(2): 581 - 588.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Chevallier, Z. Chamoun, G. Jiang, G. Prestwich, N. Sakai, S. Matile, R. G. Parton, and J. Gruenberg
Lysobisphosphatidic Acid Controls Endosomal Cholesterol Levels
J. Biol. Chem., October 10, 2008; 283(41): 27871 - 27880.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
N. H. Petersen, N. J. Faergeman, L. Yu, and D. Wustner
Kinetic imaging of NPC1L1 and sterol trafficking between plasma membrane and recycling endosomes in hepatoma cells
J. Lipid Res., September 1, 2008; 49(9): 2023 - 2037.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Yamauchi, P. C. Reid, J. B. Sperry, K. Furukawa, M. Takeya, C. C. Y. Chang, and T.-Y. Chang
Plasma Membrane Rafts Complete Cholesterol Synthesis by Participating in Retrograde Movement of Precursor Sterols
J. Biol. Chem., November 30, 2007; 282(48): 34994 - 35004.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
M. Hao, W. S. Head, S. C. Gunawardana, A. H. Hasty, and D. W. Piston
Direct Effect of Cholesterol on Insulin Secretion: A Novel Mechanism for Pancreatic {beta}-Cell Dysfunction
Diabetes, September 1, 2007; 56(9): 2328 - 2338.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Takahashi, M. Murate, M. Fukuda, S. B. Sato, A. Ohta, and T. Kobayashi
Cholesterol Controls Lipid Endocytosis through Rab11
Mol. Biol. Cell, July 1, 2007; 18(7): 2667 - 2677.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. Leyt, N. Melamed-Book, J.-P. Vaerman, S. Cohen, A. M. Weiss, and B. Aroeti
Cholesterol-sensitive Modulation of Transcytosis
Mol. Biol. Cell, June 1, 2007; 18(6): 2057 - 2071.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. D. Linder, R.-L. Uronen, M. Holtta-Vuori, P. van der Sluijs, J. Peranen, and E. Ikonen
Rab8-dependent Recycling Promotes Endosomal Cholesterol Removal in Normal and Sphingolipidosis Cells
Mol. Biol. Cell, January 1, 2007; 18(1): 47 - 56.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D. Wustner
Plasma Membrane Sterol Distribution Resembles the Surface Topography of Living Cells
Mol. Biol. Cell, January 1, 2007; 18(1): 211 - 228.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Sugii, S. Lin, N. Ohgami, M. Ohashi, C. C. Y. Chang, and T.-Y. Chang
Roles of Endogenously Synthesized Sterols in the Endocytic Pathway
J. Biol. Chem., August 11, 2006; 281(32): 23191 - 23206.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
D. Rodriguez-Agudo, S. Ren, P. B. Hylemon, R. Montanez, K. Redford, R. Natarajan, M. A. Medina, G. Gil, and W. M. Pandak
Localization of StarD5 cholesterol binding protein
J. Lipid Res., June 1, 2006; 47(6): 1168 - 1175.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
N. A. Braun, P. J. Mohler, K. H. Weisgraber, A. H. Hasty, M. F. Linton, P. G. Yancey, Y. R. Su, S. Fazio, and L. L. Swift
Intracellular trafficking of recycling apolipoprotein E in Chinese hamster ovary cells
J. Lipid Res., June 1, 2006; 47(6): 1176 - 1186.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Yu, S. Bharadwaj, J. M. Brown, Y. Ma, W. Du, M. A. Davis, P. Michaely, P. Liu, M. C. Willingham, and L. L. Rudel
Cholesterol-regulated Translocation of NPC1L1 to the Cell Surface Facilitates Free Cholesterol Uptake
J. Biol. Chem., March 10, 2006; 281(10): 6616 - 6624.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
N. H. Pipalia, A. Huang, H. Ralph, M. Rujoi, and F. R. Maxfield
Automated microscopy screening for compounds that partially revert cholesterol accumulation in Niemann-Pick C cells
J. Lipid Res., February 1, 2006; 47(2): 284 - 301.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
N. M. Urs, K. T. Jones, P. D. Salo, J. E. Severin, J. Trejo, and H. Radhakrishna
A requirement for membrane cholesterol in the {beta}-arrestin- and clathrin-dependent endocytosis of LPA1 lysophosphatidic acid receptors
J. Cell Sci., November 15, 2005; 118(22): 5291 - 5304.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. A. Churchward, T. Rogasevskaia, J. Hofgen, J. Bau, and J. R. Coorssen
Cholesterol facilitates the native mechanism of Ca2+-triggered membrane fusion
J. Cell Sci., October 15, 2005; 118(20): 4833 - 4848.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
R. S. Kiss, J. Maric, and Y. L. Marcel
Lipid efflux in human and mouse macrophagic cells: evidence for differential regulation of phospholipid and cholesterol efflux
J. Lipid Res., September 1, 2005; 46(9): 1877 - 1887.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Holtta-Vuori, F. Alpy, K. Tanhuanpaa, E. Jokitalo, A.-L. Mutka, and E. Ikonen
MLN64 Is Involved in Actin-mediated Dynamics of Late Endocytic Organelles
Mol. Biol. Cell, August 1, 2005; 16(8): 3873 - 3886.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
E. S. Ward, C. Martinez, C. Vaccaro, J. Zhou, Q. Tang, and R. J. Ober
From Sorting Endosomes to Exocytosis: Association of Rab4 and Rab11 GTPases with the Fc Receptor, FcRn, during Recycling
Mol. Biol. Cell, April 1, 2005; 16(4): 2028 - 2038.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
R. Ishitsuka, S. B. Sato, and T. Kobayashi
Imaging Lipid Rafts
J. Biochem., March 1, 2005; 137(3): 249 - 254.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
D. S. Ory
Nuclear Receptor Signaling in the Control of Cholesterol Homeostasis: Have the Orphans Found a Home?
Circ. Res., October 1, 2004; 95(7): 660 - 670.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
G. Vassiliou and R. McPherson
Role of cholesteryl ester transfer protein in selective uptake of high density lipoprotein cholesteryl esters by adipocytes
J. Lipid Res., September 1, 2004; 45(9): 1683 - 1693.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
G. Vassiliou and R. McPherson
A Novel Efflux-Recapture Process Underlies the Mechanism of High-Density Lipoprotein Cholesteryl Ester-Selective Uptake Mediated by the Low-Density Lipoprotein Receptor-Related Protein
Arterioscler. Thromb. Vasc. Biol., September 1, 2004; 24(9): 1669 - 1675.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
N. Naslavsky, R. Weigert, and J. G. Donaldson
Characterization of a Nonclathrin Endocytic Pathway: Membrane Cargo and Lipid Requirements
Mol. Biol. Cell, August 1, 2004; 15(8): 3542 - 3552.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. Rhainds, P. Bourgeois, G. Bourret, K. Huard, L. Falstrault, and L. Brissette
Localization and regulation of SR-BI in membrane rafts of HepG2 cells
J. Cell Sci., July 1, 2004; 117(15): 3095 - 3105.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
R. E. Soccio and J. L. Breslow
Intracellular Cholesterol Transport
Arterioscler. Thromb. Vasc. Biol., July 1, 2004; 24(7): 1150 - 1160.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Wang, D. Lan, W. Chen, F. Matsuura, and A. R. Tall
ATP-binding cassette transporters G1 and G4 mediate cellular cholesterol efflux to high-density lipoproteins
PNAS, June 29, 2004; 101(26): 9774 - 9779.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
O. W. Lindwasser and M. D. Resh
Human Immunodeficiency Virus Type 1 Gag Contains a Dileucine-Like Motif That Regulates Association with Multivesicular Bodies
J. Virol., June 1, 2004; 78(11): 6013 - 6023.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. B. Sato, K. Ishii, A. Makino, K. Iwabuchi, A. Yamaji-Hasegawa, Y. Senoh, I. Nagaoka, H. Sakuraba, and T. Kobayashi
Distribution and Transport of Cholesterol-rich Membrane Domains Monitored by a Membrane-impermeant Fluorescent Polyethylene Glycol-derivatized Cholesterol
J. Biol. Chem., May 28, 2004; 279(22): 23790 - 23796.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
N. Naslavsky, M. Boehm, P. S. Backlund Jr., and S. Caplan
Rabenosyn-5 and EHD1 Interact and Sequentially Regulate Protein Recycling to the Plasma Membrane
Mol. Biol. Cell, May 1, 2004; 15(5): 2410 - 2422.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Hao, S. Mukherjee, Y. Sun, and F. R. Maxfield
Effects of Cholesterol Depletion and Increased Lipid Unsaturation on the Properties of Endocytic Membranes
J. Biol. Chem., April 2, 2004; 279(14): 14171 - 14178.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
D. Wustner, M. Mondal, A. Huang, and F. R. Maxfield
Different transport routes for high density lipoprotein and its associated free sterol in polarized hepatic cells
J. Lipid Res., March 1, 2004; 45(3): 427 - 437.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
I. Coppens and K. A. Joiner
Host but Not Parasite Cholesterol Controls Toxoplasma Cell Entry by Modulating Organelle Discharge
Mol. Biol. Cell, September 1, 2003; 14(9): 3804 - 3820.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
P. L. TUMA and A. L. HUBBARD
Transcytosis: Crossing Cellular Barriers
Physiol Rev, July 1, 2003; 83(3): 871 - 932.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
S. Sugii, P. C. Reid, N. Ohgami, Y. Shimada, R. A. Maue, H. Ninomiya, Y. Ohno-Iwashita, and T.-Y. Chang
Biotinylated {theta}-toxin derivative as a probe to examine intracellular cholesterol-rich domains in normal and Niemann-Pick type C1 cells
J. Lipid Res., May 1, 2003; 44(5): 1033 - 1041.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. M. Wojtanik and L. Liscum
The Transport of Low Density Lipoprotein-derived Cholesterol to the Plasma Membrane Is Defective in NPC1 Cells
J. Biol. Chem., April 18, 2003; 278(17): 14850 - 14856.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
V. Wachtler, S. Rajagopalan, and M. K. Balasubramanian
Sterol-rich plasma membrane domains in the fission yeast Schizosaccharomyces pombe
J. Cell Sci., March 1, 2003; 116(5): 867 - 874.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Wustner, A. Herrmann, M. Hao, and F. R. Maxfield
Rapid Nonvesicular Transport of Sterol between the Plasma Membrane Domains of Polarized Hepatic Cells
J. Biol. Chem., August 9, 2002; 277(33): 30325 - 30336.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. E. Soccio, R. M. Adams, M. J. Romanowski, E. Sehayek, S. K. Burley, and J. L. Breslow
The cholesterol-regulated StarD4 gene encodes a StAR-related lipid transfer protein with two closely related homologues, StarD5 and StarD6
PNAS, May 14, 2002; 99(10): 6943 - 6948.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement