Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.C100729200 on January 7, 2002

J. Biol. Chem., Vol. 277, Issue 10, 7645-7647, March 8, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/10/7645    most recent
C100729200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rang, A.
Right arrow Articles by Will, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rang, A.
Right arrow Articles by Will, H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

ACCELERATED PUBLICATION
Antiviral Activity of Interferon-alpha against Hepatitis B Virus Can Be Studied in Non-hepatic Cells and Is Independent of MxA*

Andreas RangDagger , Michael Bruns, Tilman Heise, and Hans Will

From the Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, Martinistraße 52, 20251 Hamburg, Germany

It is well established that interferon-alpha can induce non-cytotoxic intracellular suppression of hepatitis B virus replication, but the mechanisms involved are unclear. Cell culture studies to characterize these mechanisms are restricted, in part because hepatitis B virus replicates almost exclusively in liver-derived cells. To overcome this limitation we used a cytomegalovirus promoter-controlled hepatitis B virus expression system, which leads to intracellular viral replication even in non-hepatic cell lines. In this experimental system interferon-alpha treatment specifically suppressed viral replication demonstrating that antiviral activities against hepatitis B virus are not restricted to hepatic cells. Furthermore, the interferon-inducible MxA protein was recently reported to play a key role in the antiviral action of interferon-alpha against hepatitis B virus. Our data demonstrate that interferon-alpha also suppresses hepatitis B virus replication in MxA-deficient HEp2 cells, indicating that MxA is not essential for these activities. Taken together, our data imply that the experimental approach presented can also be adapted to established cell lines which are deficient in parts of the signal transduction pathway or other elements located further downstream, providing important insights into mechanisms specifically suppressing hepatitis B virus.

* This work was supported by grants from the Bundesministerium für Bildung und Forschung and the Deutsche Forschungsgemeinschaft. The Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie is supported by the Bundesministerium für Gesundheit und the Freie and Hansestadt Hamburg.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Bundesinstitut für Gesundheitlichen Verbraucherschutz und Veterinärmedizin, Thielallee 88-92, 14195 Berlin, Germany. Tel.: 49-188-8412-3716; Fax: 49-188-8412-3635; E-mail: a.rang@bgvv.de.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 NEJMHome page
O. Schildgen, H. Sirma, A. Funk, C. Olotu, U. C. Wend, H. Hartmann, M. Helm, J. K. Rockstroh, W. R. Willems, H. Will, et al.
Variant of hepatitis B virus with primary resistance to adefovir.
N. Engl. J. Med., April 27, 2006; 354(17): 1807 - 1812.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Ehlers, S. Horke, K. Reumann, A. Rang, F. Grosse, H. Will, and T. Heise
Functional Characterization of the Interaction between Human La and Hepatitis B Virus RNA
J. Biol. Chem., October 15, 2004; 279(42): 43437 - 43447.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. Fiedler, F. Rodicker, V. Salucci, M. Lu, L. Aurisicchio, U. Dahmen, L. Jun, O. Dirsch, B. M. Putzer, F. Palombo, et al.
Helper-Dependent Adenoviral Vector-Mediated Delivery of Woodchuck-Specific Genes for Alpha Interferon (IFN-{alpha}) and IFN-{gamma}: IFN-{alpha} but Not IFN-{gamma} Reduces Woodchuck Hepatitis Virus Replication in Chronic Infection In Vivo
J. Virol., September 15, 2004; 78(18): 10111 - 10121.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. F. Wieland, R. G. Vega, R. Muller, C. F. Evans, B. Hilbush, L. G. Guidotti, J. G. Sutcliffe, P. G. Schultz, and F. V. Chisari
Searching for Interferon-Induced Genes That Inhibit Hepatitis B Virus Replication in Transgenic Mouse Hepatocytes
J. Virol., December 20, 2002; 77(2): 1227 - 1236.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement