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Originally published In Press as doi:10.1074/jbc.M109193200 on December 28, 2001

J. Biol. Chem., Vol. 277, Issue 10, 7936-7944, March 8, 2002
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The Oligogalacturonate-specific Porin KdgM of Erwinia chrysanthemi Belongs to a New Porin Family*

Nicolas BlotDagger , Catherine Berrier§, Nicole Hugouvieux-Cotte-PattatDagger , Alexandre Ghazi§, and Guy CondemineDagger

From the Dagger  Unité de Microbiologie et Génétique, Composante INSA, UMR-CNRS 5122, INSA, Bâtiment Louis Pasteur, 11 Avenue Jean Capelle, 69621 Villeurbanne Cedex, and the § Groupe Canaux Ioniques, UMR-CNRS 8619, Bâtiment 430, Université Paris-Sud, 91405 Orsay Cedex, France

The phytopathogenic Gram-negative bacteria Erwinia chrysanthemi secretes pectinases, which are able to degrade the pectic polymers of plant cell walls, and uses the degradation products as a carbon source for growth. We characterized a major outer membrane protein, KdgM, whose synthesis is strongly induced in the presence of pectic derivatives. The corresponding gene was characterized. Analysis of transcriptional fusions showed that the kdgM expression is controlled by the general repressor of pectinolytic genes, KdgR, by the repressor of hexuronate catabolism genes, ExuR, by the pectinase gene repressor, PecS, and by catabolite repression via the cyclic AMP receptor protein (CRP) transcriptional activator. A kdgM mutant is unable to grow on oligogalacturonides longer than trimers, and its virulence is affected. Electrophysiological experiments with planar lipid bilayers showed that KdgM behaves like a voltage-dependent porin that is slightly selective for anions and that exhibits fast block in the presence of trigalacturonate. In contrast to most porins, KdgM seems to be monomeric. KdgM has no homology with currently known porins, but proteins similar to KdgM are present in several bacteria. Therefore, these proteins might constitute a new family of porin channels.

* This work was supported by grants from the CNRS and from the Ministère de la Recherche.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Unité de Microbiologie et Génétique, Composante INSA, UMR-CNRS 5122, INSA, Bâtiment Louis Pasteur, 11 Ave. Jean Capelle, 69621 Villeurbanne Cedex, France. Tel.: 33-472438088; Fax: 33-472438714; E-mail: condemin@insa-lyon.fr.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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