|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 10, 7970-7978, March 8, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the The bactericidal/permeability increasing (BPI)
and lipopolysaccharide (LPS)-binding (LBP) proteins are
closely related two-domain proteins in which LPS binding is mediated by
the NH2-terminal domain. To further define the role
of the COOH-terminal domain of these proteins in delivery of LPS to
specific host acceptors, we have compared interactions of LBP, BPI,
LBPN-BPIC (NH2-terminal domain of
LBP, COOH-terminal domain of BPI), and
BPIN-LBPC with purified 3H-LPS and,
subsequently, with purified leukocytes and soluble (s)CD14. The
COOH-terminal domain of LBP promotes delivery of LPS to CD14 on both
polymorphonuclear leukocytes and monocytes resulting in cell
activation. In the presence of Ca2+ and Mg2+,
LBP and BPI each promote aggregation of LPS to protein-LPS aggregates of increased size (apparent Mr > 20 × 106 Da), but only LPS associated with LBP and
BPIN-LBPC is disaggregated in the presence of
CD14. BPI and LBPN-BPIC promote apparently CD14-independent LPS association to monocytes without cell activation. These findings demonstrate that the carboxyl-terminal domain of these
closely related endotoxin-binding proteins dictates the route and host
responses to complexes they form with endotoxin.
The Carboxyl-terminal Domain of Closely Related Endotoxin-binding
Proteins Determines the Target of Protein-Lipopolysaccharide
Complexes*
,,
**
Department of Medicine, New York University
School of Medicine, New York, New York 10016, the Departments of
Biochemistry, ¶ Microbiology, and § Internal
Medicine, Division of Infectious Diseases, Inflammation Program,
University of Iowa, and Veterans Affairs Medical Center,
Iowa City, Iowa 52242
*
This work was supported by United States Public Health
Service Grants DK05472 and PO144642 (to J. W.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  I. Wittmann, M. Schonefeld, D. Aichele, G. Groer, A. Gessner, and M. Schnare Murine Bactericidal/Permeability-Increasing Protein Inhibits the Endotoxic Activity of Lipopolysaccharide and Gram-Negative Bacteria J. Immunol., June 1, 2008; 180(11): 7546 - 7552. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Satoh, S. Ando, T. Shinoda, and M. Yamazaki Clearance of bacterial lipopolysaccharides and lipid A by the liver and the role of arginino-succinate synthase Innate Immunity, February 1, 2008; 14(1): 51 - 60. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Teghanemt, P. Prohinar, T. L. Gioannini, and J. P. Weiss Transfer of Monomeric Endotoxin from MD-2 to CD14: CHARACTERIZATION AND FUNCTIONAL CONSEQUENCES J. Biol. Chem., December 14, 2007; 282(50): 36250 - 36256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Schultz, J. Hume, D. S. Zhang, T. L. Gioannini, and J. P. Weiss A Novel Role for the Bactericidal/Permeability Increasing Protein in Interactions of Gram-Negative Bacterial Outer Membrane Blebs with Dendritic Cells J. Immunol., August 15, 2007; 179(4): 2477 - 2484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. Gioannini, A. Teghanemt, D. Zhang, P. Prohinar, E. N. Levis, R. S. Munford, and J. P. Weiss Endotoxin-binding Proteins Modulate the Susceptibility of Bacterial Endotoxin to Deacylation by Acyloxyacyl Hydrolase J. Biol. Chem., March 16, 2007; 282(11): 7877 - 7884. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Canny, E. Cario, A. Lennartsson, U. Gullberg, C. Brennan, O. Levy, and S. P. Colgan Functional and biochemical characterization of epithelial bactericidal/permeability-increasing protein Am J Physiol Gastrointest Liver Physiol, March 1, 2006; 290(3): G557 - G567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Satoh, T. Iwahori, N. Sugawara, and M. Yamazaki Liver argininosuccinate synthase binds to bacterial lipopolysaccharides and lipid A and inactivates their biological activities Innate Immunity, February 1, 2006; 12(1): 21 - 38. [Abstract] [PDF]
|
|
|
|
 |
|
 R. K. M. Choy, J. M. Kemner, and J. H. Thomas Fluoxetine-Resistance Genes in Caenorhabditis elegans Function in the Intestine and May Act in Drug Transport Genetics, February 1, 2006; 172(2): 885 - 892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Eckert, I. Wittmann, M. Rollinghoff, A. Gessner, and M. Schnare Endotoxin-Induced Expression of Murine Bactericidal Permeability/Increasing Protein Is Mediated Exclusively by Toll/IL-1 Receptor Domain-Containing Adaptor Inducing IFN-{beta}-Dependent Pathways J. Immunol., January 1, 2006; 176(1): 522 - 528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. McMichael, A. Roghanian, L. Jiang, R. Ramage, and J.-M. Sallenave The Antimicrobial Antiproteinase Elafin Binds to Lipopolysaccharide and Modulates Macrophage Responses Am. J. Respir. Cell Mol. Biol., May 1, 2005; 32(5): 443 - 452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.L. Gioannini, A. Teghanemt, DeS. Zhang, E.N. Levis, and J.P. Weiss Monomeric endotoxin:protein complexes are essential for TLR4-dependent cell activation Innate Immunity, April 1, 2005; 11(2): 117 - 123. [Abstract] [PDF]
|
|
|
|
|
|
C. Habich, K. Kempe, R. van der Zee, R. Rumenapf, H. Akiyama, H. Kolb, and V. Burkart Heat Shock Protein 60: Specific Binding of Lipopolysaccharide J. Immunol., February 1, 2005; 174(3): 1298 - 1305. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Levy Antimicrobial proteins and peptides: anti-infective molecules of mammalian leukocytes J. Leukoc. Biol., November 1, 2004; 76(5): 909 - 925. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. P. Jia, J. N. Kline, A. Penisten, M. A. Apicella, T. L. Gioannini, J. Weiss, and P. B. McCray Jr. Endotoxin responsiveness of human airway epithelia is limited by low expression of MD-2 Am J Physiol Lung Cell Mol Physiol, August 1, 2004; 287(2): L428 - L437. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Gioannini, A. Teghanemt, D. Zhang, N. P. Coussens, W. Dockstader, S. Ramaswamy, and J. P. Weiss Isolation of an endotoxin-MD-2 complex that produces Toll-like receptor 4-dependent cell activation at picomolar concentrations PNAS, March 23, 2004; 101(12): 4186 - 4191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Campos, A. R. Abreu, M. C. Nlend, M. A. Cobas, G. E. Conner, and P. L. Whitney Purification and Characterization of PLUNC from Human Tracheobronchial Secretions Am. J. Respir. Cell Mol. Biol., February 1, 2004; 30(2): 184 - 192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. Gioannini, A. Teghanemt, K. A. Zarember, and J. P. Weiss Regulation of interactions of endotoxin with host cells Innate Immunity, December 1, 2003; 9(6): 401 - 408. [Abstract] [PDF]
|
|
|
|
 |
|
 E.E. LeClair Four Reasons to Consider a Novel Class of Innate Immune Molecules in the Oral Epithelium Journal of Dental Research, December 1, 2003; 82(12): 944 - 950. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. Gioannini, D. Zhang, A. Teghanemt, and J. P. Weiss An Essential Role for Albumin in the Interaction of Endotoxin with Lipopolysaccharide-binding Protein and sCD14 and Resultant Cell Activation J. Biol. Chem., November 27, 2002; 277(49): 47818 - 47825. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |