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Originally published In Press as doi:10.1074/jbc.M110849200 on December 31, 2001

J. Biol. Chem., Vol. 277, Issue 10, 7989-7995, March 8, 2002
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Role of the Ada2 and Ada3 Transcriptional Coactivators in Histone Acetylation*

Ramakrishnan BalasubramanianDagger §, Marilyn G. Pray-Grant§, William SelleckDagger , Patrick A. Grant||, and Song TanDagger **

From the Dagger  Center for Gene Regulation, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802-1014 and the  Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908-0733

Previous studies have shown that the transcriptional coactivator protein Gcn5 functions as a catalytic histone acetyltransferase (HAT). In this work, we examine the roles of the Ada2 and Ada3 coactivator proteins that are functionally linked to Gcn5. We show that yeast Ada2, Ada3, and Gcn5 form a catalytic core of the ADA and Spt-Ada-Gcn5-acetyltransferase HAT complexes, which is necessary and sufficient in vitro for nucleosomal HAT activity and lysine specificity of the intact HAT complexes. We also demonstrate that Ada3 is necessary for Gcn5-dependent nucleosomal HAT activity in yeast extracts. Our results suggest that Ada2 potentiates the Gcn5 catalytic activity and that Ada3 facilitates nucleosomal acetylation and an expanded lysine specificity.

* This work was supported in part by National Institutes of Health Grants DK-58646-01 (to P. A. G.) and GM-60489 (to S. T.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ These authors contributed equally to this work.

|| Recipient of a Burroughs Wellcome career development award.

** Pew Scholar in the biomedical sciences. To whom correspondence should be addressed: Center for Gene Regulation, Dept. of Biochemistry and Molecular Biology, 108 Althouse Laboratory, Pennsylvania State University, University Park, PA 16802-1014. E-mail: sxt30@psu.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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