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Originally published In Press as doi:10.1074/jbc.M109508200 on December 19, 2001

J. Biol. Chem., Vol. 277, Issue 10, 8202-8208, March 8, 2002
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Isolation and Characterization of XKaiso, a Transcriptional Repressor That Associates with the Catenin Xp120ctn in Xenopus laevis*

Si Wan KimDagger , Xiang FangDagger , Hong JiDagger , Alicia F. PaulsonDagger , Juliet M. Daniel§, Malgorzata Ciesiolka, Frans van Roy, and Pierre D. McCreaDagger ||**

From the Dagger  Department of Biochemistry and Molecular Biology, || Program in Genes and Development, The University of Texas M.D. Anderson Cancer Center, Houston, Texas 77030, the § Department of Biology, McMaster University, Hamilton, Ontario L8S 4K1, Canada, and the  Department of Molecular Biology, VIB-University of Gent, Ledeganckstraat 35, B-9000 Gent, Belgium

The Armadillo family of catenin proteins function in multiple capacities including cadherin-mediated cell-cell adhesion and nuclear signaling. The newest catenin, p120ctn, differs from the classical catenins and binds to the membrane-proximal domain of cadherins. Recently, a novel transcription factor Kaiso was found to interact with p120ctn, suggesting that p120ctn also possesses a nuclear function. We isolated the Xenopus homolog of Kaiso, XKaiso, from a Xenopus stage 17 cDNA library. XKaiso contains an amino-terminal BTB/POZ domain and three carboxyl-terminal zinc fingers. The XKaiso transcript was present maternally and expressed throughout early embryonic development. XKaiso's spatial expression was defined via in situ hybridization and was found localized to the brain, eye, ear, branchial arches, and spinal cord. Co-immunoprecipitation of Xenopus p120ctn and XKaiso demonstrated their mutual association, whereas related experiments employing differentially epitope-tagged XKaiso constructs suggest that XKaiso additionally self-associates. Finally, reporter assays employing a chimera of XKaiso fused to the GAL4 DNA binding domain indicate that XKaiso is a transcriptional repressor. These data suggest that XKaiso functions throughout development and that its repressor functions may be most apparent in the context of neural tissues. The significance of the XKaiso-p120ctn interaction has yet to be determined, but it may include transducing information from cadherin-mediated cell-cell contacts to transcriptional processes within the nucleus.

* This work was supported in part by National Institutes of Health RO1 Grant GM 52112, a Pharmacia/Monsanto Research Award, a Kleberg Foundation Award, and Cancer Center Support Grant Funds CCSG-CA 16672 (to P. D. M.) and by the Fund for Scientific Research-Flanders and Fortis Verzekeringen (Belgium) (to F. V. R.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AF420316.

** To whom correspondence should be addressed: Dept. of Biochemistry and Molecular Biology, Box 117, The University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030. Tel.: 713-792-8979; Fax: 713-791-9478; E-mail: pmccrea@odin.mdacc.tmc.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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