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J. Biol. Chem., Vol. 277, Issue 10, 8449-8456, March 8, 2002
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From the Low voltage activated, voltage-operated
Ca2+ channels are expressed in rodent male germ cells
and are believed to be pivotal in induction of the acrosome reaction in
mouse spermatozoa. However, in humans, very little is known about
expression of voltage-operated Ca2+ channels in male germ
cells or their function. We have used reverse transcription-polymerase
chain reaction, in situ hybridization, and patch clamp
recording to investigate the expression of low voltage activated
voltage-operated Ca2+ channels in human male germ cells. We
report that full-length transcripts for both The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AJ420779.
Identification and Localization of T-type Voltage-operated
Calcium Channel Subunits in Human Male Germ Cells
EXPRESSION OF MULTIPLE ISOFORMS*
,§,¶,
,,
School of Biosciences, University of
Birmingham, Birmingham B15 2TT, United Kingdom, CNRS,
Commissariat à l'Energie Atomique/Grenoble DBMS-CIS, 17 Rue des Martyrs, Grenoble, 38054 France, and the
** Reproductive Biology and Genetics Research Group, Assisted
Conception Unit, Birmingham Women's Hospital,
Birmingham B15 2TG, United Kingdom
1G and
1H low voltage activated channel subunits are expressed
in human testis. Multiple isoforms of 1G are present in
the testis and at least two isoforms of 1H, including a
splice variant not previously described in the human. Transcripts for
all the isoforms of both 1G and 1H were
detected by reverse transcription-polymerase chain reaction on
mRNA isolated from human spermatogenic cells. In situ
hybridization for 1G and 1H localized
transcripts both in germ cells and in other cell types in the testis.
Within the seminiferous tubules, 1H was detected primarily in germ cells. Using the whole cell patch clamp technique, we
detected T-type voltage-operated Ca2+ channel currents in
isolated human male germ cells, although the current amplitude and
frequency of occurrence were low in comparison to the occurrence of
T-currents in murine male germ cells. We conclude that low
voltage activated voltage-operated Ca2+ channels are
expressed in cells of the human male germ line.
*
This work was supported in part by a National Health Service
Locally Organized Research Scheme project grant.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Present address: Dept. of Obstetrics and Gynecology,
Yale University School of Medicine, New Haven, CT 06520-8063.
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