JBC PeproTech; Our Business is Cytokines!

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M110615200 on December 10, 2001

J. Biol. Chem., Vol. 277, Issue 10, 8658-8666, March 8, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
277/10/8658    most recent
M110615200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fuss, J.
Right arrow Articles by Linn, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fuss, J.
Right arrow Articles by Linn, S.

Human DNA Polymerase epsilon  Colocalizes with Proliferating Cell Nuclear Antigen and DNA Replication Late, but Not Early, in S Phase*,

Jill FussDagger and Stuart Linn§

From the Department of Molecular and Cell Biology, Division of Biochemistry and Molecular Biology, University of California, Berkeley, California 94720-3206

DNA polymerase epsilon  (pol epsilon ) has been implicated in DNA replication, DNA repair, and cell cycle control, but its precise roles are unclear. When the subcellular localization of human pol epsilon  was examined by indirect immunofluorescence, pol epsilon  appeared in discrete nuclear foci that colocalized with proliferating cell nuclear antigen (PCNA) foci and sites of DNA synthesis only late in S phase. Early in S phase, pol epsilon  foci were adjacent to PCNA foci. In contrast to PCNA foci that were only present in S phase, pol epsilon  foci were present throughout mitosis and the G1 phase of cycling cells. It is hypothesized from these observations that pol epsilon  and PCNA have separate but associated functions early in S phase and that pol epsilon  participates with PCNA in DNA replication late in S phase.

* This work was supported by National Institutes of Health Grants 1RO1GM30415 and P30ES08196.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains a supplemental video which shows a series of confocal planes that progress from the lower to the uppermost planes of the cells shown in Fig. 5. A mitotic (upper right) and an S phase (lower left) cell are stained for pol epsilon  p261 (red), BrdUrd (green), and DAPI (blue).

Dagger Current address: Lawrence Berkeley National Laboratory, 1 Cyclotron Road, MS 74-157, Berkeley, CA 94720.

§ To whom correspondence should be addressed: Dept. of Molecular and Cell Biology, Division of Biochemistry and Molecular Biology, 229 Stanley Hall, University of California, Berkeley, CA 94720-3206. Tel.: 510-642-7583; Fax: 510-643-9290; E-mail: slinn@socrates.berkeley.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
J. Villegas, V. Burzio, C. Villota, E. Landerer, R. Martinez, M. Santander, R. Martinez, R. Pinto, M. I. Vera, E. Boccardo, et al.
Expression of a novel non-coding mitochondrial RNA in human proliferating cells
Nucleic Acids Res., December 18, 2007; 35(21): 7336 - 7347.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
O. Chilkova, P. Stenlund, I. Isoz, C. M. Stith, P. Grabowski, E.-B. Lundstrom, P. M. Burgers, and E. Johansson
The eukaryotic leading and lagging strand DNA polymerases are loaded onto primer-ends via separate mechanisms but have comparable processivity in the presence of PCNA
Nucleic Acids Res., October 8, 2007; 35(19): 6588 - 6597.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
Z. F. Pursell, I. Isoz, E.-B. Lundstrom, E. Johansson, and T. A. Kunkel
Yeast DNA Polymerase {epsilon} Participates in Leading-Strand DNA Replication
Science, July 6, 2007; 317(5834): 127 - 130.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Zhang, Y. Zhou, S. Trusa, X. Meng, E. Y. C. Lee, and M. Y. W. T. Lee
A Novel DNA Damage Response: RAPID DEGRADATION OF THE p12 SUBUNIT OF DNA POLYMERASE {delta}
J. Biol. Chem., May 25, 2007; 282(21): 15330 - 15340.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Z. F. Pursell, I. Isoz, E.-B. Lundstrom, E. Johansson, and T. A. Kunkel
Regulation of B family DNA polymerase fidelity by a conserved active site residue: characterization of M644W, M644L and M644F mutants of yeast DNA polymerase {varepsilon}
Nucleic Acids Res., May 14, 2007; 35(9): 3076 - 3086.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Tsubota, R. Tajima, K. Ode, H. Kubota, N. Fukuhara, T. Kawabata, S. Maki, and H. Maki
Double-stranded DNA Binding, an Unusual Property of DNA Polymerase {epsilon}, Promotes Epigenetic Silencing in Saccharomyces cerevisiae
J. Biol. Chem., October 27, 2006; 281(43): 32898 - 32908.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
Y. Chu, R. Piper, S. Richardson, Y. Watanabe, P. Patel, and D. D. Heistad
Endocytosis of Extracellular Superoxide Dismutase Into Endothelial Cells: Role of the Heparin-Binding Domain
Arterioscler. Thromb. Vasc. Biol., September 1, 2006; 26(9): 1985 - 1990.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
J. Rong, J. E. Bowers, S. R. Schulze, V. N. Waghmare, C. J. Rogers, G. J. Pierce, H. Zhang, J. C. Estill, and A. H. Paterson
Comparative genomics of Gossypium and Arabidopsis: Unraveling the consequences of both ancient and recent polyploidy
Genome Res., September 1, 2005; 15(9): 1198 - 1210.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
I. Hernandez-Munoz, A. H. Lund, P. van der Stoop, E. Boutsma, I. Muijrers, E. Verhoeven, D. A. Nusinow, B. Panning, Y. Marahrens, and M. van Lohuizen
From the Cover: Stable X chromosome inactivation involves the PRC1 Polycomb complex and requires histone MACROH2A1 and the CULLIN3/SPOP ubiquitin E3 ligase
PNAS, May 24, 2005; 102(21): 7635 - 7640.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
S.-I. Hiraga, A. Hagihara-Hayashi, T. Ohya, and A. Sugino
DNA polymerases {alpha}, {delta}, and {varepsilon} localize and function together at replication forks in Saccharomyces cerevisiae
Genes Cells, April 1, 2005; 10(4): 297 - 309.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. V. Mitkova, E. E. Biswas-Fiss, and S. B. Biswas
Modulation of DNA Synthesis in Saccharomyces cerevisiae Nuclear Extract by DNA Polymerases and the Origin Recognition Complex
J. Biol. Chem., February 25, 2005; 280(8): 6285 - 6292.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
T. Fukui, K. Yamauchi, T. Muroya, M. Akiyama, H. Maki, A. Sugino, and S. Waga
Distinct roles of DNA polymerases delta and epsilon at the replication fork in Xenopus egg extracts
Genes Cells, March 1, 2004; 9(3): 179 - 191.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. Iida and H. Araki
Noncompetitive Counteractions of DNA Polymerase {varepsilon} and ISW2/yCHRAC for Epigenetic Inheritance of Telomere Position Effect in Saccharomyces cerevisiae
Mol. Cell. Biol., January 1, 2004; 24(1): 217 - 227.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. V. Shcherbakova, Y. I. Pavlov, O. Chilkova, I. B. Rogozin, E. Johansson, and T. A. Kunkel
Unique Error Signature of the Four-subunit Yeast DNA Polymerase {epsilon}
J. Biol. Chem., October 31, 2003; 278(44): 43770 - 43780.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
W. Feng, L. Rodriguez-Menocal, G. Tolun, and G. D'Urso
Schizosacchromyces pombe Dpb2 Binds to Origin DNA Early in S Phase and Is Required for Chromosomal DNA Replication
Mol. Biol. Cell, August 1, 2003; 14(8): 3427 - 3436.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. Asahara, Y. Li, J. Fuss, D. S. Haines, N. Vlatkovic, M. T. Boyd, and S. Linn
Stimulation of human DNA polymerase {epsilon} by MDM2
Nucleic Acids Res., May 1, 2003; 31(9): 2451 - 2459.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
D. S.F. Biard, L. Miccoli, E. Despras, F. Harper, E. Pichard, C. Creminon, and J. F. Angulo
Participation of kin17 Protein in Replication Factories and in Other DNA Transactions Mediated by High Molecular Weight Nuclear Complexes
Mol. Cancer Res., May 1, 2003; 1(7): 519 - 531.
[Abstract] [Full Text] [PDF]

Home page
 Sci Aging Knowl EnvironHome page
P. V. Shcherbakova, K. Bebenek, and T. A. Kunkel
Functions of Eukaryotic DNA Polymerases
Sci. Aging Knowl. Environ., February 26, 2003; 2003(8): re3 - 3.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
V. Bergoglio, C. Bavoux, V. Verbiest, J.-S. Hoffmann, and C. Cazaux
Localisation of human DNA polymerase {kappa} to replication foci
J. Cell Sci., January 12, 2002; 115(23): 4413 - 4418.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.