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J. Biol. Chem., Vol. 277, Issue 11, 8835-8840, March 15, 2002
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From the Departments of Biochemistry and Biophysics and
Pharmaceutical Chemistry, University of California at San
Francisco, San Francisco, California 94143-0448
An Escherichia coli open
reading frame, ygcA, was identified as a putative 23 S
ribosomal RNA 5-methyluridine methyltransferase (Gustafsson, C.,
Reid, R., Greene, P. J., and Santi, D. V. (1996) Nucleic Acids Res. 24, 3756-3762). We have cloned,
expressed, and purified the 50-kDa protein encoded by ygcA.
The purified enzyme catalyzed the AdoMet-dependent
methylation of 23 S rRNA but did not act upon 16 S rRNA or tRNA. A high
performance liquid chromatography-based nucleoside analysis
identified the reaction product as 5-methyluridine. The enzyme
specifically methylated U1939 as determined by a nuclease protection
assay and by methylation assays using site-specific mutants of 23 S
rRNA. A 40-nucleotide 23 S rRNA fragment (nucleotide 1930-1969) also
served as an efficient substrate for the enzyme. The apparent
Km values for the 40-mer RNA oligonucleotide and
AdoMet were 3 and 26 µM, respectively, and the apparent
kcat was 0.06 s
Characterization of the 23 S Ribosomal RNA m5U1939
Methyltransferase from Escherichia coli*
,, and
1. The enzyme
contains two equivalents of iron/monomer and has a sequence motif
similar to a motif found in iron-sulfur proteins. We propose to name
this gene rumA and accordingly name the protein product as RumA for RNA uridine methyltransferase.
*
This work was supported by the United States Public Health
Service, National Institutes of Health Grant GM51232 (to R. M. S.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Present address: Kosan Biosciences, Inc., 3832 Bay Center Place,
Hayward, CA 94545.
§
To whom correspondence should be addressed: Dept. of Biochemistry
and Biophysics, University of California at San Francisco, San
Francisco, California 94143-0448. Tel.: 415-476-4224; Fax: 415-476-1902; E-mail: stroud@msg.ucsf.edu.
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