|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 11, 8854-8860, March 15, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Chronic leptin administration reduces
triacylglycerol content in skeletal muscle. We hypothesized that
chronic leptin treatment, within physiologic limits, would reduce the
fatty acid uptake capacity of red and white skeletal muscle due to a
reduction in transport protein expression (fatty acid translocase
(FAT/CD36) and plasma membrane-associated fatty acid-binding protein
(FABPpm)) at the plasma membrane. Female Sprague-Dawley rats were
infused for 2 weeks with leptin (0.5 mg/kg/day) using subcutaneously
implanted miniosmotic pumps. Control and pair-fed animals received
saline-filled implants. Leptin levels were significantly elevated
(~4-fold; p < 0.001) in treated animals, whereas
pair-fed treated animals had reduced serum leptin levels (approximately
Chronic Leptin Administration Decreases Fatty Acid Uptake and
Fatty Acid Transporters in Rat Skeletal Muscle*
§,,
,, Department of Human Biology and Nutritional
Sciences, University of Guelph, Ontario N1G 2W1, Canada, ¶ Glaxo
SmithKline, Miami, Florida 33134, the Thrombosis Research
Laboratory, Otsuka Maryland Research Institute, Rockville, Maryland
20850, the ** Department of Physiology, Maastricht
University, 6200 MD Maastricht, The Netherlands, and the
§§ Department of Kinesiology, University of
Waterloo, Waterloo, Ontario N2L 3G1, Canada
2-fold; p < 0.01) relative to controls. Palmitate
transport rates into giant sarcolemmal vesicles were reduced following
leptin treatment in both red (45%) and white (84%) skeletal
muscle compared with control and pair-fed animals (p < 0.05). Leptin treatment reduced FAT mRNA (red, 70%, p < 0.001; white, 48%, p < 0.01)
and FAT/CD36 protein expression (red, 32%; p < 0.05) in whole muscle homogenates, whereas FABPpm mRNA and protein
expression were unaltered. However, in leptin-treated animals plasma
membrane fractions of both FAT/CD36 and FABPpm protein expression were
significantly reduced in red (28 and 34%, respectively) and white
(44 and 56%, respectively) muscles (p < 0.05).
Across all experimental treatments and muscles, palmitate uptake by
giant sarcolemmal vesicles was highly correlated with the plasma
membrane FAT/CD36 protein (r = 0.88, p < 0.01) and plasma membrane FABPpm protein
(r = 0.94, p < 0.01). These studies provide the first evidence that protein-mediated long chain fatty acid
transport is subject to long term regulation by leptin.
*
This work was supported by grants from the Canadian
Institutes of Health Research (to A. B.) and the Natural Sciences and Engineering Research Council of Canada (to D. J. D. and A. B.) and
by Netherlands Heart Foundation Grant D98.012 (to J. F. C. G. and
J. J. F. P. L.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
A Dekker postdoctoral fellow of the Netherlands Heart Foundation.
¶¶
To whom correspondence should be addressed:
Dept. of Kinesiology, University of Waterloo, Waterloo, Ontario N2L
3G1, Canada. Tel.: 519-888-1211 (ext. 5214); Fax: 519-746-6776;
E-mail: abonen@healthy.uwaterloo.ca.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. B. Jorgensen, J. Honeyman, J. S. Oakhill, D. Fazakerley, J. Stockli, B. E. Kemp, and G. R. Steinberg Oligomeric resistin impairs insulin and AICAR-stimulated glucose uptake in mouse skeletal muscle by inhibiting GLUT4 translocation Am J Physiol Endocrinol Metab, July 1, 2009; 297(1): E57 - E66. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. P. Holloway, C. R. Benton, K. L. Mullen, Y. Yoshida, L. A. Snook, X.-X. Han, J. F. C. Glatz, J. J. F. P. Luiken, J. Lally, D. J. Dyck, et al. In obese rat muscle transport of palmitate is increased and is channeled to triacylglycerol storage despite an increase in mitochondrial palmitate oxidation Am J Physiol Endocrinol Metab, April 1, 2009; 296(4): E738 - E747. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. P Turcotte and J. S Fisher Skeletal Muscle Insulin Resistance: Roles of Fatty Acid Metabolism and Exercise Physical Therapy, November 1, 2008; 88(11): 1279 - 1296. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Smith, X. Su, R. El-Maghrabi, P. D. Stahl, and N. A. Abumrad Opposite Regulation of CD36 Ubiquitination by Fatty Acids and Insulin: EFFECTS ON FATTY ACID UPTAKE J. Biol. Chem., May 16, 2008; 283(20): 13578 - 13585. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Dube, B. A. Bhatt, N. Dedousis, A. Bonen, and R. M. O'Doherty Leptin, skeletal muscle lipids, and lipid-induced insulin resistance Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2007; 293(2): R642 - R650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-X. Han, A. Chabowski, N. N. Tandon, J. Calles-Escandon, J. F. C. Glatz, J. J. F. P. Luiken, and A. Bonen Metabolic challenges reveal impaired fatty acid metabolism and translocation of FAT/CD36 but not FABPpm in obese Zucker rat muscle Am J Physiol Endocrinol Metab, August 1, 2007; 293(2): E566 - E575. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. P. Holloway, J. Lally, J. G. Nickerson, H. Alkhateeb, L. A. Snook, G. J. F. Heigenhauser, J. Calles-Escandon, J. F. C. Glatz, J. J. F. P. Luiken, L. L. Spriet, et al. Fatty acid binding protein facilitates sarcolemmal fatty acid transport but not mitochondrial oxidation in rat and human skeletal muscle J. Physiol., July 1, 2007; 582(1): 393 - 405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bonen, X.-X. Han, D. D. J. Habets, M. Febbraio, J. F. C. Glatz, and J. J. F. P. Luiken A null mutation in skeletal muscle FAT/CD36 reveals its essential role in insulin- and AICAR-stimulated fatty acid metabolism Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1740 - E1749. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Guerra, A. Santana, T. Fuentes, S. Delgado-Guerra, A. Cabrera-Socorro, C. Dorado, and J. A. L. Calbet Leptin receptors in human skeletal muscle J Appl Physiol, May 1, 2007; 102(5): 1786 - 1792. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bonen, A. Chabowski, J. J. F. P Luiken, and J. F. C. Glatz Mechanisms and Regulation of Protein-Mediated Cellular Fatty Acid Uptake: Molecular, Biochemical, and Physiological Evidence Physiology, February 1, 2007; 22(1): 15 - 28. [Full Text] [PDF]
|
|
|
|
 |
|
 G. R. Steinberg, A. J. McAinch, M. B. Chen, P. E. O'Brien, J. B. Dixon, D. Cameron-Smith, and B. E. Kemp The Suppressor of Cytokine Signaling 3 Inhibits Leptin Activation of AMP-Kinase in Cultured Skeletal Muscle of Obese Humans J. Clin. Endocrinol. Metab., September 1, 2006; 91(9): 3592 - 3597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Chabowski, J. C. Chatham, N. N. Tandon, J. Calles-Escandon, J. F. C. Glatz, J. J. F. P. Luiken, and A. Bonen Fatty acid transport and FAT/CD36 are increased in red but not in white skeletal muscle of ZDF rats Am J Physiol Endocrinol Metab, September 1, 2006; 291(3): E675 - E682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. R. Benton, D. P. Y. Koonen, J. Calles-Escandon, N. N. Tandon, J. F. C. Glatz, J. J. F. P. Luiken, J. J. Heikkila, and A. Bonen Differential effects of contraction and PPAR agonists on the expression of fatty acid transporters in rat skeletal muscle J. Physiol., May 15, 2006; 573(1): 199 - 210. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Kiens Skeletal Muscle Lipid Metabolism in Exercise and Insulin Resistance Physiol Rev, January 1, 2006; 86(1): 205 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Coles, J. Litt, H. Hatta, and A. Bonen Exercise rapidly increases expression of the monocarboxylate transporters MCT1 and MCT4 in rat muscle J. Physiol., November 15, 2004; 561(1): 253 - 261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Chabowski, S. L. M. Coort, J. Calles-Escandon, N. N. Tandon, J. F. C. Glatz, J. J. F. P. Luiken, and A. Bonen Insulin stimulates fatty acid transport by regulating expression of FAT/CD36 but not FABPpm Am J Physiol Endocrinol Metab, October 1, 2004; 287(4): E781 - E789. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. P. Y. Koonen, C. R. Benton, Y. Arumugam, N. N. Tandon, J. Calles-Escandon, J. F. C. Glatz, J. J. F. P. Luiken, and A. Bonen Different mechanisms can alter fatty acid transport when muscle contractile activity is chronically altered Am J Physiol Endocrinol Metab, June 1, 2004; 286(6): E1042 - E1049. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Clarke, D. Miskovic, X.-X. Han, J. Calles-Escandon, J. F. C. Glatz, J. J. F. P. Luiken, J. J. Heikkila, and A. Bonen Overexpression of membrane-associated fatty acid binding protein (FABPpm) in vivo increases fatty acid sarcolemmal transport and metabolism Physiol Genomics, March 12, 2004; 17(1): 31 - 37. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Steinberg, A. C. Smith, S. Wormald, P. Malenfant, C. Collier, and D. J. Dyck Endurance training partially reverses dietary-induced leptin resistance in rodent skeletal muscle Am J Physiol Endocrinol Metab, January 1, 2004; 286(1): E57 - E63. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Z. Tucker and L. P. Turcotte Aging is associated with elevated muscle triglyceride content and increased insulin-stimulated fatty acid uptake Am J Physiol Endocrinol Metab, October 1, 2003; 285(4): E827 - E835. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Binas, X.-X. Han, E. Erol, J. J. F. P. Luiken, J. F. C. Glatz, D. J. Dyck, R. Motazavi, P. J. Adihetty, D. A. Hood, and A. Bonen A null mutation in H-FABP only partially inhibits skeletal muscle fatty acid metabolism Am J Physiol Endocrinol Metab, September 1, 2003; 285(3): E481 - E489. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Fan, M. W. Bradbury, and P. D. Berk Leptin and Insulin Modulate Nutrient Partitioning and Weight Loss in ob/ob Mice through Regulation of Long-Chain Fatty Acid Uptake by Adipocytes J. Nutr., September 1, 2003; 133(9): 2707 - 2715. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. F. P. Luiken, Y. Arumugam, R. C. Bell, J. Calles-Escandon, N. N. Tandon, J. F. C. Glatz, and A. Bonen Changes in fatty acid transport and transporters are related to the severity of insulin deficiency Am J Physiol Endocrinol Metab, September 1, 2002; 283(3): E612 - E621. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |