JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M110761200 on December 20, 2001

J. Biol. Chem., Vol. 277, Issue 11, 8898-8905, March 15, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/11/8898    most recent
M110761200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wu, Y.
Right arrow Articles by Burris, T. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wu, Y.
Right arrow Articles by Burris, T. P.

Requirement of Helix 1 and the AF-2 Domain of the Thyroid Hormone Receptor for Coactivation by PGC-1*

Yifei Wu, Philippe Delerive, William W. Chin, and Thomas P. BurrisDagger

From Gene Regulation, Bone, and Inflammation Research, Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, Indiana 46285

Although PGC-1 (peroxisome proliferator-activated receptor-gamma coactivator-1) has been previously shown to enhance thyroid hormone receptor (TR)/retinoid X receptor-mediated ucp-1 gene expression in a ligand-induced manner in rat fibroblast cells, the precise mechanism of PGC-1 modulation of TR function has yet to be determined. In this study, we show that PGC-1 can potentiate TR-mediated transactivation of reporter genes driven by natural thyroid hormone response elements both in a ligand-dependent and ligand-independent manner and that the extent of coactivation is a function of the thyroid hormone response element examined. Our data also show that PGC-1 stimulation of TR activity in terms of Gal4 DNA-binding domain fusion is strictly ligand-dependent. In addition, an E457A AF-2 mutation had no effect on the ligand-induced PGC-1 enhancement of TR activity, indicating that the conserved charged residue in AF-2 is not essential for this PGC-1 function. Furthermore, GST pull-down and mammalian two-hybrid assays demonstrated that the PGC-1 LXXLL motif is required for ligand-induced PGC-1/TR interaction. This agonist-dependent PGC-1/TR interaction also requires both helix 1 and the AF-2 region of the TR ligand-binding domain. Taken together, these results support the notion that PGC-1 is a bona fide TR coactivator and that PGC-1 modulates TR activity via a mechanism different from that utilized with peroxisome proliferator activator receptor-gamma .

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Gene Regulation, DC 0434, Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, IN 46285. Tel.: 317-433-4962; Fax: 317-276-1414; E-mail: Burris_Thomas_P@lilly.com.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.


This article has been cited by other articles:


Home page
 EndocrinologyHome page
Y.-Y. Liu, R. S. Heymann, F. Moatamed, J. J. Schultz, D. Sobel, and G. A. Brent
A Mutant Thyroid Hormone Receptor {alpha} Antagonizes Peroxisome Proliferator-Activated Receptor {alpha} Signaling in Vivo and Impairs Fatty Acid Oxidation
Endocrinology, March 1, 2007; 148(3): 1206 - 1217.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
F. Flamant, K. Gauthier, and J. Samarut
Thyroid Hormones Signaling Is Getting More Complex: STORMs Are Coming
Mol. Endocrinol., February 1, 2007; 21(2): 321 - 333.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S. Gaillard, M. A. Dwyer, and D. P. McDonnell
Definition of the Molecular Basis for Estrogen Receptor-Related Receptor-{alpha}-Cofactor Interactions
Mol. Endocrinol., January 1, 2007; 21(1): 62 - 76.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. Oberkofler, K. Klein, T. K. Felder, F. Krempler, and W. Patsch
Role of Peroxisome Proliferator-Activated Receptor-{gamma} Coactivator-1{alpha} in the Transcriptional Regulation of the Human Uncoupling Protein 2 Gene in INS-1E Cells
Endocrinology, February 1, 2006; 147(2): 966 - 976.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
R. S. Savkur, K. S. Bramlett, K. R. Stayrook, S. Nagpal, and T. P. Burris
Coactivation of the Human Vitamin D Receptor by the Peroxisome Proliferator-Activated Receptor {gamma} Coactivator-1 {alpha}
Mol. Pharmacol., August 1, 2005; 68(2): 511 - 517.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Iordanidou, E. Aggelidou, C. Demetriades, and M. Hadzopoulou-Cladaras
Distinct Amino Acid Residues May Be Involved in Coactivator and Ligand Interactions in Hepatocyte Nuclear Factor-4{alpha}
J. Biol. Chem., June 10, 2005; 280(23): 21810 - 21819.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
J. M. R. Moore and R. K. Guy
Coregulator Interactions with the Thyroid Hormone Receptor
Mol. Cell. Proteomics, April 1, 2005; 4(4): 475 - 482.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
R. S. Savkur, J. S. Thomas, K. S. Bramlett, Y. Gao, L. F. Michael, and T. P. Burris
Ligand-Dependent Coactivation of the Human Bile Acid Receptor FXR by the Peroxisome Proliferator-Activated Receptor {gamma} Coactivator-1{alpha}
J. Pharmacol. Exp. Ther., January 1, 2005; 312(1): 170 - 178.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Zhang, K. Ma, S. Song, Marshall. B. Elam, G. A. Cook, and E. A. Park
Peroxisomal Proliferator-activated Receptor-{gamma} Coactivator-1{alpha} (PGC-1{alpha}) Enhances the Thyroid Hormone Induction of Carnitine Palmitoyltransferase I (CPT-I{alpha})
J. Biol. Chem., December 24, 2004; 279(52): 53963 - 53971.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. M. R. Moore, S. J. Galicia, A. C. McReynolds, N.-H. Nguyen, T. S. Scanlan, and R. K. Guy
Quantitative Proteomics of the Thyroid Hormone Receptor-Coregulator Interactions
J. Biol. Chem., June 25, 2004; 279(26): 27584 - 27590.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
L. K. Russell, C. M. Mansfield, J. J. Lehman, A. Kovacs, M. Courtois, J. E. Saffitz, D. M. Medeiros, M. L. Valencik, J. A. McDonald, and D. P. Kelly
Cardiac-Specific Induction of the Transcriptional Coactivator Peroxisome Proliferator-Activated Receptor {gamma} Coactivator-1{alpha} Promotes Mitochondrial Biogenesis and Reversible Cardiomyopathy in a Developmental Stage-Dependent Manner
Circ. Res., March 5, 2004; 94(4): 525 - 533.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S. Sanyal, J. Matthews, D. Bouton, H.-J. Kim, H.-S. Choi, E. Treuter, and J.-A. Gustafsson
Deoxyribonucleic Acid Response Element-Dependent Regulation of Transcription by Orphan Nuclear Receptor Estrogen Receptor-Related Receptor {gamma}
Mol. Endocrinol., February 1, 2004; 18(2): 312 - 325.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. L. Moore, E. A. Park, and J. B. McMillin
Upstream Stimulatory Factor Represses the Induction of Carnitine Palmitoyltransferase-Ibeta Expression by PGC-1
J. Biol. Chem., May 2, 2003; 278(19): 17263 - 17268.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Shiraki, N. Sakai, E. Kanaya, and H. Jingami
Activation of Orphan Nuclear Constitutive Androstane Receptor Requires Subnuclear Targeting by Peroxisome Proliferator-activated Receptor gamma Coactivator-1alpha . A POSSIBLE LINK BETWEEN XENOBIOTIC RESPONSE AND NUTRITIONAL STATE
J. Biol. Chem., March 21, 2003; 278(13): 11344 - 11350.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Wu, W. W. Chin, Y. Wang, and T. P. Burris
Ligand and Coactivator Identity Determines the Requirement of the Charge Clamp for Coactivation of the Peroxisome Proliferator-activated Receptor gamma
J. Biol. Chem., February 28, 2003; 278(10): 8637 - 8644.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
B. R. Huber, M. Desclozeaux, B. L. West, S. T. Cunha-Lima, H. T. Nguyen, J. D. Baxter, H. A. Ingraham, and R. J. Fletterick
Thyroid Hormone Receptor-{beta} Mutations Conferring Hormone Resistance and Reduced Corepressor Release Exhibit Decreased Stability in the N-Terminal Ligand-Binding Domain
Mol. Endocrinol., January 1, 2003; 17(1): 107 - 116.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Ichida, S. Nemoto, and T. Finkel
Identification of a Specific Molecular Repressor of the Peroxisome Proliferator-activated Receptor gamma Coactivator-1 alpha (PGC-1alpha )
J. Biol. Chem., December 20, 2002; 277(52): 50991 - 50995.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. M. Huss, R. P. Kopp, and D. P. Kelly
Peroxisome Proliferator-activated Receptor Coactivator-1alpha (PGC-1alpha ) Coactivates the Cardiac-enriched Nuclear Receptors Estrogen-related Receptor-alpha and -gamma . IDENTIFICATION OF NOVEL LEUCINE-RICH INTERACTION MOTIF WITHIN PGC-1alpha
J. Biol. Chem., October 18, 2002; 277(43): 40265 - 40274.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Desclozeaux, I. N. Krylova, F. Horn, R. J. Fletterick, and H. A. Ingraham
Phosphorylation and Intramolecular Stabilization of the Ligand Binding Domain in the Nuclear Receptor Steroidogenic Factor 1
Mol. Cell. Biol., October 15, 2002; 22(20): 7193 - 7203.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Oberkofler, H. Esterbauer, V. Linnemayr, A. D. Strosberg, F. Krempler, and W. Patsch
Peroxisome Proliferator-activated Receptor (PPAR) gamma Coactivator-1 Recruitment Regulates PPAR Subtype Specificity
J. Biol. Chem., May 3, 2002; 277(19): 16750 - 16757.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.