Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M111482200 on December 28, 2001

J. Biol. Chem., Vol. 277, Issue 11, 8928-8933, March 15, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/11/8928    most recent
M111482200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Suaud, L.
Right arrow Articles by Kleyman, T. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Suaud, L.
Right arrow Articles by Kleyman, T. R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Genistein Restores Functional Interactions between Delta F508-CFTR and ENaC in Xenopus Oocytes*

Laurence SuaudDagger §, Jinqing Li||, Qinshi Jiang||, Ronald C. RubensteinDagger **Dagger Dagger , and Thomas R. Kleyman§§¶¶

From the Dagger  Division of Pulmonary Medicine, Children's Hospital of Philadelphia, Departments of || Medicine and ** Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, 19104 and §§ Renal-Electrolyte Division, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261

The cystic fibrosis transmembrane conductance regulator (CFTR), in addition to its Cl- channel properties, has regulatory interactions with other epithelial ion channels including the epithelial Na+ channel (ENaC). Both the open probability and surface expression of wild type CFTR Cl- channels are increased significantly when CFTR is co-expressed in Xenopus oocytes with alpha beta gamma -ENaC, and conversely, the activity of ENaC is inhibited following wild type CFTR activation. Using the Xenopus oocyte expression system, a lack of functional regulatory interactions between Delta F508-CFTR and ENaC was observed following activation of Delta F508-CFTR by forskolin and isobutylmethylxanthine (IBMX). Whole cell currents in oocytes expressing ENaC alone decreased in response to genistein but increased in response to a combination of forskolin and IBMX followed by genistein. In contrast, ENaC currents in oocytes co-expressing ENaC and Delta F508-CFTR remained stable following stimulation with forskolin/IBMX/genistein. Furthermore, co-expression of Delta F508-CFTR with ENaC enhanced the forskolin/IBMX/genistein-mediated activation of Delta F508-CFTR. Our data suggest that genistein restores regulatory interactions between Delta F508-CFTR and ENaC and that combinations of protein repair agents, such as 4-phenylbutyrate and genistein, may be necessary to restore Delta F508-CFTR function in vivo.

* This work was supported by Grants DK56305 and DK58046 from the National Institutes of Health (to T. R. K. and to R. C. R., respectively).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Recipient of a postdoctoral fellowship award from the Pennsylvania/Delaware Chapter of the American Heart Association.

Recipient of a postdoctoral fellowship award from the Cystic Fibrosis Foundation.

Dagger Dagger Recipient of a Cystic Fibrosis Foundation Leroy Matthews Individual Physician Scientist Award.

¶¶ To whom correspondence should be addressed: Renal-Electrolyte Division, A919 Scaife Hall, 3550 Terrace St., Pittsburgh, PA 15261. Tel.: 412-647-3121; Fax: 412-648-9166; E-mail: kleyman@pitt.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Suaud, W. Yan, M. D. Carattino, A. Robay, T. R. Kleyman, and R. C. Rubenstein
Regulatory interactions of N1303K-CFTR and ENaC in Xenopus oocytes: evidence that chloride transport is not necessary for inhibition of ENaC
Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1553 - C1561.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Suaud, W. Yan, and R. C. Rubenstein
Abnormal regulatory interactions of I148T-CFTR and the epithelial Na+ channel in Xenopus oocytes
Am J Physiol Cell Physiol, January 1, 2007; 292(1): C603 - C611.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Su, Q. Li, K. Shrestha, E. Cormet-Boyaka, L. Chen, P. R. Smith, E. J. Sorscher, D. J. Benos, S. Matalon, and H.-L. Ji
Interregulation of Proton-gated Na+ Channel 3 and Cystic Fibrosis Transmembrane Conductance Regulator
J. Biol. Chem., December 1, 2006; 281(48): 36960 - 36968.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. B. Goldfarb, O. B. Kashlan, J. N. Watkins, L. Suaud, W. Yan, T. R. Kleyman, and R. C. Rubenstein
Differential effects of Hsc70 and Hsp70 on the intracellular trafficking and functional expression of epithelial sodium channels
PNAS, April 11, 2006; 103(15): 5817 - 5822.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
G. Nagel, P. Barbry, H. Chabot, E. Brochiero, K. Hartung, and R. Grygorczyk
CFTR fails to inhibit the epithelial sodium channel ENaC expressed in Xenopus laevis oocytes
J. Physiol., May 1, 2005; 564(3): 671 - 682.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Yan, F. F. Samaha, M. Ramkumar, T. R. Kleyman, and R. C. Rubenstein
Cystic Fibrosis Transmembrane Conductance Regulator Differentially Regulates Human and Mouse Epithelial Sodium Channels in Xenopus Oocytes
J. Biol. Chem., May 28, 2004; 279(22): 23183 - 23192.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
R.C. Boucher
New concepts of the pathogenesis of cystic fibrosis lung disease
Eur. Respir. J., January 1, 2004; 23(1): 146 - 158.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Suaud, M. Carattino, T. R. Kleyman, and R. C. Rubenstein
Genistein Improves Regulatory Interactions between G551D-Cystic Fibrosis Transmembrane Conductance Regulator and the Epithelial Sodium Channel in Xenopus Oocytes
J. Biol. Chem., December 20, 2002; 277(52): 50341 - 50347.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement