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J. Biol. Chem., Vol. 277, Issue 11, 9088-9095, March 15, 2002
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From the Department of Biochemistry, Stanford University School of
Medicine, Stanford, California 94305-5307
Dynacortin is a novel protein that was discovered
in a genetic suppressor screen of a Dictyostelium
discoideum cytokinesis-deficient mutant cell line devoid of the
cleavage furrow actin bundling protein, cortexillin I. While dynacortin
is highly enriched in the cortex, particularly in cell-surface
protrusions, it is excluded from the cleavage furrow cortex during
cytokinesis. Here, we describe the biochemical characterization of this
new protein. Purified dynacortin is an 80-kDa dimer with a large 5.7-nm
Stokes radius. Dynacortin cross-links actin filaments into parallel
arrays with a mole ratio of one dimer to 1.3 actin monomers and a 3.1 µM Kd. Using total internal
reflection fluorescence microscopy, GFP-dynacortin and the actin
bundling protein coronin-GFP are seen to concentrate in highly dynamic
cortical structures with assembly and disassembly half-lives of about
15 s. These results indicate that cells have evolved different
actin-filament cross-linking proteins with complementary cellular
distributions that collaborate to orchestrate complex cell shape changes.
Dynacortin Is a Novel Actin Bundling Protein That Localizes to
Dynamic Actin Structures*
,
*
This work was supported by a Burroughs Wellcome Career
Development Award (to D. N. R.), a Helen Hay Whitney
postdoctoral fellowship (to R. S. R.), and National
Institutes of Health Grant GM40509 (to J. A. S.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence may be addressed. Current
address: Dept. of Cell Biology, Johns Hopkins University School of
Medicine, 725 N. Wolfe St., Baltimore, MD 21205. Tel.: 410-502-2850;
E-mail: Douglas.Robinson@jhu.edu.
§
To whom correspondence may be addressed. E-mail:
jspudich@cmgm.stanford.edu.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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