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Originally published In Press as doi:10.1074/jbc.M109955200 on December 13, 2001

J. Biol. Chem., Vol. 277, Issue 11, 9335-9341, March 15, 2002
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Absence of Erythrogenesis and Vasculogenesis in Plcg1-deficient Mice*

Hong-Jun LiaoDagger , Tsutomu Kume§, Catriona McKay||, Ming-Jiang Xu**, James N. Ihle, and Graham CarpenterDagger **Dagger Dagger

From the Departments of Dagger  Biochemistry, § Cell Biology, and ** Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 and the  Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, Tennessee 38105

Mice nullizygous for Plcg1 cease growing at early to mid-gestation. An examination of carefully preserved wild-type embryos shows clear evidence of erythropoiesis, but erythropoiesis is not evident in Plcg1 nullizygous embryos at the same stage. The analyses of embryonic materials demonstrate that in the absence of Plcg1, erythroid progenitors cannot be detected in the yolk sac or embryo body by three different assays, burst-forming units, colony-forming units, and analysis for the developmental marker Ter119. However, non-erythroid granulocyte/macrophage colonies are produced by Plcg1 null embryos. Further analysis of these embryos demonstrates significantly diminished vasculogenesis in Plcg1 nullizygous embryos based on the lack of expression of the endothelial marker platelet endothelial cell adhesion molecule-1. In addition, Plcg1 nullizygous embryos express a greatly reduced level of vascular endothelial growth factor receptor-2/Flk-1, consistent with significantly impaired vasculogenesis and erythropoiesis. Interestingly, these early embryos do express phospholipase C-gamma 2, however, it is unable to substitute for the absence of phospholipase C-gamma 1, which can be detected in its tyrosine-phosphorylated state.

* This work was supported by National Institutes of Health Grant CA75195.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|| Present address: PPS International Communications Ltd., Worthing, UK.

Dagger Dagger To whom correspondence should be addressed: Dept. of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232-0146. Tel.: 615-322-6678; Fax: 615-322-2931; E-mail: graham.carpenter@mcmail.vanderbilt.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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