Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M106116200 on January 14, 2002

J. Biol. Chem., Vol. 277, Issue 12, 10614-10625, March 22, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/12/10614    most recent
M106116200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, C.-Y.
Right arrow Articles by Lu, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, C.-Y.
Right arrow Articles by Lu, B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Regulation of Neuromuscular Synapse Development by Glial Cell Line-derived Neurotrophic Factor and Neurturin*

Chang-Yu WangDagger §, Feng YangDagger , Xiang-Ping HeDagger , Hyun-Soo JeDagger §, Jian-Zheng ZhouDagger , Kary EckermannDagger , David KawamuraDagger , Linyin FengDagger ||, Liya Shen**, and Bai LuDagger Dagger Dagger

From the Dagger  Unit on Synapse Development and Plasticity, NICHD, National Institutes of Health, Bethesda, Maryland 20892, the § Genetics Graduate Program, George Washington University, Washington, D.C. 20052, the ** Laboratory of Cellular Carcinogenesis and Tumor Promotion, NCI, National Institutes of Health, Bethesda, Maryland 20892, and the || Institute of Neuroscience, Chinese Academy of Sciences, Shanghai, China 200031

Glial cell line-derived neurotrophic factor (GDNF) is known for its potent effect on neuronal survival, but its role in the development and function of synapses is not well studied. Using Xenopus nerve-muscle co-cultures, we show that GDNF and its family member neurturin (NRTN) facilitate the development of the neuromuscular junction (NMJ). Long-term application of GDNF significantly increased the total length of neurites in the motoneurons. GDNF also caused an increase in the number and the size of synaptic vesicle clustering, as demonstrated by synaptobrevin-GFP fluorescent imaging, and FM dye staining. Electrophysiological experiments revealed two effects of GDNF on synaptic transmission at NMJ. First, GDNF markedly increased the frequency of spontaneous transmission and decreased the variability of evoked transmission, suggesting an enhancement of transmitter secretion. Second, GDNF elicited a small increase in the quantal size, without affecting the average rise and decay times of synaptic currents. Imaging analysis showed that the size of acetylcholine receptor clusters at synapses increased in muscle cells overexpressing GDNF. Neurturin had very similar effects as GDNF. These results suggest that GDNF and NRTN are new neuromodulators that regulate the development of the neuromuscular synapse through both pre- and postsynaptic mechanisms.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

These authors contributed equally to the results of this work.

Dagger Dagger To whom correspondence should be addressed: Unit on Synapse Development and Plasticity, NICHD, National Institutes of Health, Bldg. 49, Rm. 6A-80, 49 Convent Dr., MSC4480, Bethesda, MD 20892-4480. Tel.: 301-435-2970; Fax: 301-496-1777; E-mail: lub@codon.nih.gov.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Neurosci.Home page
C. Baudet, E. Pozas, I. Adameyko, E. Andersson, J. Ericson, and P. Ernfors
Retrograde Signaling onto Ret during Motor Nerve Terminal Maturation
J. Neurosci., January 23, 2008; 28(4): 963 - 975.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. B. Rind, R. Butowt, and C. S. von Bartheld
Synaptic Targeting of Retrogradely Transported Trophic Factors in Motoneurons: Comparison of Glial Cell Line-Derived Neurotrophic Factor, Brain-Derived Neurotrophic Factor, and Cardiotrophin-1 with Tetanus Toxin
J. Neurosci., January 19, 2005; 25(3): 539 - 549.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. M. Myers and L. M. Mulligan
The RET Receptor Is Linked to Stress Response Pathways
Cancer Res., July 1, 2004; 64(13): 4453 - 4463.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. B. Peng, J.-F. Yang, Z. Dai, C. W. Lee, H. W. Hung, Z. H. Feng, and C.-P. Ko
Differential Effects of Neurotrophins and Schwann Cell-Derived Signals on Neuronal Survival/Growth and Synaptogenesis
J. Neurosci., June 15, 2003; 23(12): 5050 - 5060.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
S. Gianino, J. R. Grider, J. Cresswell, H. Enomoto, and R. O. Heuckeroth
GDNF availability determines enteric neuron number by controlling precursor proliferation
Development, May 15, 2003; 130(10): 2187 - 2198.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement