|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 12, 10683-10690, March 22, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Physiological Chemistry, School of
Veterinary Medicine Hannover, Hannover D-30559, Germany
The apical sorting of human intestinal dipeptidyl
peptidase IV (DPPIV) occurs through complex N-linked and
O-linked carbohydrates. Inhibition of O-linked
glycosylation by
benzyl-N-acetyl-
Intestinal Dipeptidyl Peptidase IV Is Efficiently Sorted to the
Apical Membrane through the Concerted Action of N- and
O-Glycans as Well as Association with Lipid
Microdomains*
-D-galactosaminide affects
significantly the sorting behavior of DPPIV in intestinal Caco-2 and
HT-29 cells. However, random delivery to the apical and basolateral
membranes and hence a more drastic effect on the sorting of DPPIV in
both cell types is only observed when, in addition to
O-glycans, the processing of N-glycans is
affected by swainsonine, an inhibitor of mannosidase II. Together the
data indicate that both types of glycosylation are critical components of the apical sorting signal of DPPIV. The sorting mechanism of DPPIV implicates its association with detergent-insoluble membrane microdomains containing cholesterol and sphingolipids, whereas an
efficient association largely depends on the presence of a fully
complex N- and O-linked glycosylated DPPIV.
Interestingly, cholesterol is a more critical component in this context
than sphingolipids, because cholesterol depletion by 
-cyclodextrin affects the detergent solubility and the sorting behavior of DPPIV more
strongly than fumonisin, an inhibitor of sphingolipid synthesis.
*
This work was supported by Grant Na 331/1-2 from the
Deutsche Forschungsgemeinschaft, Bonn, Germany (to H. Y. N.), and the Sonderforschungsbereich 280.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom all correspondence should be addressed: Dept. of
Physiological Chemistry, School of Veterinary Medicine, Hannover, Bünteweg 17, D-30559 Hannover, Germany. Tel.: 49-511-9538780; Fax: 49-511- 9538585; E-mail: Hassan.Naim@tiho-hannover.de.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Delacour, A. Koch, W. Ackermann, I. E.-L. Parco, H.-P. Elsasser, F. Poirier, and R. Jacob Loss of galectin-3 impairs membrane polarisation of mouse enterocytes in vivo J. Cell Sci., February 15, 2008; 121(4): 458 - 465. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. R. S. Tagaram, G. Wang, T. M. Umstead, A. N. Mikerov, N. J. Thomas, G. R. Graff, J. C. Hess, M. J. Thomassen, M. S. Kavuru, D. S. Phelps, et al. Characterization of a human surfactant protein A1 (SP-A1) gene-specific antibody; SP-A1 content variation among individuals of varying age and pulmonary health Am J Physiol Lung Cell Mol Physiol, May 1, 2007; 292(5): L1052 - L1063. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Parry, F. G. Hanisch, S.-H. Leir, M. Sutton-Smith, H. R. Morris, A. Dell, and A. Harris N-Glycosylation of the MUC1 mucin in epithelial cells and secretions Glycobiology, July 1, 2006; 16(7): 623 - 634. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Keiser, M. Alfalah, M. J. Propsting, D. Castelletti, and H. Y. Naim Altered Folding, Turnover, and Polarized Sorting Act in Concert to Define a Novel Pathomechanism of Congenital Sucrase-Isomaltase Deficiency J. Biol. Chem., May 19, 2006; 281(20): 14393 - 14399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Castelletti, G. Fracasso, M. Alfalah, S. Cingarlini, M. Colombatti, and H. Y. Naim Apical Transport and Folding of Prostate-specific Membrane Antigen Occurs Independent of Glycan Processing J. Biol. Chem., February 10, 2006; 281(6): 3505 - 3512. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Potter, R. P. Hughey, and O. A. Weisz Role of N- and O-glycans in polarized biosynthetic sorting Am J Physiol Cell Physiol, January 1, 2006; 290(1): C1 - C10. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Alfalah, G. Wetzel, I. Fischer, R. Busche, E. E. Sterchi, K.-P. Zimmer, H.-P. Sallmann, and H. Y. Naim A Novel Type of Detergent-resistant Membranes May Contribute to an Early Protein Sorting Event in Epithelial Cells J. Biol. Chem., December 30, 2005; 280(52): 42636 - 42643. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Delacour, V. Gouyer, J.-P. Zanetta, H. Drobecq, E. Leteurtre, G. Grard, O. Moreau-Hannedouche, E. Maes, A. Pons, S. Andre, et al. Galectin-4 and sulfatides in apical membrane trafficking in enterocyte-like cells J. Cell Biol., May 9, 2005; 169(3): 491 - 501. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Popik and T. M. Alce CD4 Receptor Localized to Non-raft Membrane Microdomains Supports HIV-1 Entry: IDENTIFICATION OF A NOVEL RAFT LOCALIZATION MARKER IN CD4 J. Biol. Chem., January 2, 2004; 279(1): 704 - 712. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. K. Nyasae, A. L. Hubbard, and P. L. Tuma Transcytotic Efflux from Early Endosomes Is Dependent on Cholesterol and Glycosphingolipids in Polarized Hepatic Cells Mol. Biol. Cell, July 1, 2003; 14(7): 2689 - 2705. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Hoekstra, O. Maier, J. M. van der Wouden, T. A. Slimane, and S. C. D. van IJzendoorn Membrane dynamics and cell polarity: the role of sphingolipids J. Lipid Res., May 1, 2003; 44(5): 869 - 877. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |