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Originally published In Press as doi:10.1074/jbc.M200240200 on January 14, 2002

J. Biol. Chem., Vol. 277, Issue 12, 9736-9740, March 22, 2002
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Reanalysis of ATP11B, a Type IV P-type ATPase*

Margaret S. HalleckDagger §, Robert A. SchlegelDagger , and Patrick L. Williamson

From the Dagger  Department of Biochemistry and Molecular Biology, Penn State University, University Park, Pennsylvania 16802 and  Department of Biology, Amherst College, Amherst, Massachusetts 01002

P-type ATPases are a venerable family of ATP-dependent ion transporters. Recently, evidence was presented that a rabbit gene in the type IV subfamily of P-type ATPases was missing a transmembrane helix (transmembrane domain 4) thought to be critical for ion transport, a deletion that would place the two major catalytic loops of the enzyme on opposite sides of the membrane. It was proposed that the resulting protein was a RING finger-binding protein that targets transcription factors to specific domains within the nucleus. From analysis of human genomic sequence data, it is shown here that the region containing transmembrane domain 4, corresponding to exon 12, is present in the human homolog of the gene, ATP11B. PCR analysis indicates that the predominant Atp11b transcripts in a rabbit cDNA library and in a mouse cDNA library also contain exon 12. The results suggest that the transcript proposed to encode the RING finger-binding protein is a minor rabbit-specific splice variant. The ATP11B gene thus may not encode a protein with a function radically different from that of other P-type ATPase transporters.

* This work was supported in part by National Institutes of Health Grant R01 GM55862 (to R. A. S. and P. L. W.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AY066014 and AY069938.

§ To whom correspondence should be addressed: Dept. of Biochemistry and Molecular Biology, 410 South Frear Laboratory, Penn State University, University Park, PA 16802. Tel.: 814-863-1767; Fax: 814-863-7024; E-mail: msh4@psu.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.


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