|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 12, 9853-9864, March 22, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the To evaluate possible fibrogenic effects of
CYP2E1-dependent generation of reactive oxygen species, a
model was developed using co-cultures of HepG2 cells, which do (E47
cells) or do not (C34 cells) express cytochrome P450 2E1 (CYP2E1) with
stellate cells. There was an increase in intra- and extracellular
H2O2, lipid peroxidation, and collagen
type I protein in stellate cells co-cultured with E47 cells compared
with stellate cells alone or co-cultured with C34 cells. The increase
in collagen was prevented by antioxidants and a CYP2E1 inhibitor.
CYP3A4 did not mimic the stimulatory effects found with CYP2E1.
Collagen mRNA levels remained unchanged, and pulse-chase analysis
indicated similar half-lives of collagen I protein between both
co-cultures. However, collagen protein synthesis was increased in E47
co-culture. Hepatocytes from pyrazole-treated rats (with high levels of
CYP2E1) induced collagen protein in primary stellate cells, and
antioxidants and CYP2E1 inhibitors blocked this effect. These results
suggest that increased translation of collagen mRNA by
CYP2E1-derived reactive oxygen species is responsible for the increase
in collagen protein produced by the E47 co-culture. These co-culture
models may be useful for understanding the impact of CYP2E1-derived ROS
on stellate cell function and activation.
Cytochrome P450 2E1-derived Reactive Oxygen Species Mediate
Paracrine Stimulation of Collagen I Protein Synthesis by Hepatic
Stellate Cells*
,¶
Department of Pharmacology and Biological
Chemistry and the § Department of Medicine and Liver
Diseases, Mount Sinai School of Medicine,
New York, New York 10029
*
These studies were supported by United States Public Health
Service Grants AA03312 and AA06610 from the National Institute on
Alcohol Abuse and Alcoholism (to A. I. C.), DK 37340 from NIDDK, National Institutes of Health (to S. L. F.), and the Revson
Fellowship and a Grant from the Alcohol Beverage Medical Research
Foundation (to N. N.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  L. D. DeLeve, X. Wang, G. C. Kanel, R. D. Atkinson, and R. S. McCuskey Prevention of Hepatic Fibrosis in a Murine Model of Metabolic Syndrome with Nonalcoholic Steatohepatitis Am. J. Pathol., October 1, 2008; 173(4): 993 - 1001. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Dostalek, K. D. Hardy, G. L. Milne, J. D. Morrow, C. Chen, F. J. Gonzalez, J. Gu, X. Ding, D. A. Johnson, J. A. Johnson, et al. Development of Oxidative Stress by Cytochrome P450 Induction in Rodents Is Selective for Barbiturates and Related to Loss of Pyridine Nucleotide-dependent Protective Systems J. Biol. Chem., June 20, 2008; 283(25): 17147 - 17157. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Friedman Hepatic Stellate Cells: Protean, Multifunctional, and Enigmatic Cells of the Liver Physiol Rev, January 1, 2008; 88(1): 125 - 172. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. G. Lechuga, Z. H. Hernandez-Nazara, E. Hernandez, M. Bustamante, G. Desierto, A. Cotty, N. Dharker, M. Choe, and M. Rojkind PI3K is involved in PDGF-beta receptor upregulation post-PDGF-BB treatment in mouse HSC Am J Physiol Gastrointest Liver Physiol, December 1, 2006; 291(6): G1051 - G1061. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Y. Chang, C.-Y. Han, T. N. Wight, and A. Chait Antioxidants Inhibit the Ability of Lysophosphatidylcholine to Regulate Proteoglycan Synthesis Arterioscler. Thromb. Vasc. Biol., March 1, 2006; 26(3): 494 - 500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Schmidt, F. Gerlach, A. Avivi, T. Laufs, S. Wystub, J. C. Simpson, E. Nevo, S. Saaler-Reinhardt, S. Reuss, T. Hankeln, et al. Cytoglobin Is a Respiratory Protein in Connective Tissue and Neurons, Which Is Up-regulated by Hypoxia J. Biol. Chem., February 27, 2004; 279(9): 8063 - 8069. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. F. Schwabe, R. Bataller, and D. A. Brenner Human hepatic stellate cells express CCR5 and RANTES to induce proliferation and migration Am J Physiol Gastrointest Liver Physiol, November 1, 2003; 285(5): G949 - G958. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-W. Chow, M. T. Herrera Abreu, T. Suzuki, and G. P. Downey Oxidative Stress and Acute Lung Injury Am. J. Respir. Cell Mol. Biol., October 1, 2003; 29(4): 427 - 431. [Full Text] [PDF]
|
|
|
|
|
|
N. Nieto and A. I. Cederbaum Increased Sp1-dependent Transactivation of the LAMgamma 1 Promoter in Hepatic Stellate Cells Co-cultured with HepG2 Cells Overexpressing Cytochrome P450 2E1 J. Biol. Chem., April 18, 2003; 278(17): 15360 - 15372. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. C. Chung, S. H. Kim, M. G. Lee, and S. G. Kim Increase in Urea in Conjunction with L-Arginine Metabolism in the Liver Leads to Induction of Cytochrome P450 2E1 (CYP2E1): The Role of Urea in CYP2E1 Induction by Acute Renal Failure Drug Metab. Dispos., June 1, 2002; 30(6): 739 - 746. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |