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J. Biol. Chem., Vol. 277, Issue 12, 9911-9919, March 22, 2002
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From the The E2F transcription factors are key components
of the cyclin D/retinoblastoma/E2F pathway. Here we demonstrate that
Arabidopsis thaliana contains six functional AtE2F genes
that are all expressed in cell suspension culture but show different
patterns of expression during cell cycle progression. According to
their structural and functional features, the six AtE2Fs can be divided
into two distinct groups; although the three members of the first
group, AtE2Fa, AtE2Fb and AtE2Fc, possess all the conserved domains
found in other plant and animal E2Fs, the remaining AtE2Fs are novel
proteins, which reveal a duplication of the DNA binding domain but lack any other conserved region. Furthermore, the AtE2Fs of the first group
are functional transcription factors that in association with AtDP
proteins can recognize specifically an E2F cis-element and
can transactivate an E2F-responsive reporter gene in plant cells. In
contrast, the AtE2Fs of the second group can bind specifically the E2F
site without interacting with DP partners but cannot activate gene
expression and, instead, are able to inhibit E2F-dependent activation of gene expression in Arabidopsis cells. These
findings suggest distinctive roles for the plant E2F proteins
and point to a complex concerted regulation of
E2F-dependent gene expression in plant cells.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AJ417834-AJ417837.
The E2F Family of Transcription Factors from Arabidopsis
thaliana
NOVEL AND CONSERVED COMPONENTS OF THE RETINOBLASTOMA/E2F PATHWAY
IN PLANTS*
,,,, and
Department of Genetics and Microbiology,
University of Pavia, Via Ferrata 1, 27100 Pavia, Italy, the
§ Institute of Plant Biotechnology, University of Paris-XI,
91400 Orsay, France, and the ¶ Department of Botany and Plant
Ecology, University of Sassari, Via Muroni 25, 07100 Sassari, Italy
*
This work was supported by grants "Programmi di Ricerca
Scientifica di Interesse Nazionale" from the Ministero
dell'Università e della Ricerca Scientifica e Tecnologica and
"Progetto di Ricerca di Ateneo" from the University of Pavia.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.:
39-79-228645; Fax: 39-79-233600; E-mail: albani@ssmain.uniss.it.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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