|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 13, 10834-10841, March 29, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Departments of Reductions in activity of SSIII, the major
isoform of starch synthase responsible for amylopectin synthesis in the
potato tuber, result in fissuring of the starch granules. To discover the causes of the fissuring, and thus to shed light on factors that
influence starch granule morphology in general, SSIII antisense lines
were compared with lines with reductions in the major granule-bound isoform of starch synthase (GBSS) and lines with reductions in activity
of both SSIII and GBSS (SSIII/GBSS antisense lines). This revealed that
fissuring resulted from the activity of GBSS in the SSIII antisense
background. Control (untransformed) lines and GBSS and SSIII/GBSS
antisense lines had unfissured granules. Starch analyses showed that
granules from SSIII antisense tubers had a greater number of
long glucan chains than did granules from the other lines, in the form
of larger amylose molecules and a unique fraction of very long
amylopectin chains. These are likely to result from increased flux
through GBSS in SSIII antisense tubers, in response to the elevated
content of ADP-glucose in these tubers. It is proposed that the
long glucan chains disrupt organization of the semi-crystalline parts
of the matrix, setting up stresses in the matrix that lead to fissuring.
Role of Granule-bound Starch Synthase in Determination of
Amylopectin Structure and Starch Granule Morphology in Potato*
,,,,,
,**
Metabolic Biology and
§ Cell and Developmental Biology, John Innes Centre, Colney
Lane, Norwich NR4 7UH, United Kingdom, the ¶ Department of
Physics, University of Cambridge, Cavendish Laboratory, Madingley Rd.,
Cambridge CB3 0HE, United Kingdom, and the Max-Planck Institute
of Molecular Plant Physiology, Am Mühlenberg 1, Golm D-14476,
Germany
*
This work was supported by a competitive Strategic Grant
from the Biotechnology and Biological Sciences Research Council (BBSRC, United Kingdom), to the John Innes Centre, by BBSRC Grant D08036, by a
BBSRC research studentship (to E. P.), by a grant from the European
Union (Framework Programme IV, project CT95-0568, to A. E.),
and by Plant Biosciences Ltd., Norwich, UK.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  I. Hanashiro, K. Itoh, Y. Kuratomi, M. Yamazaki, T. Igarashi, J.-i. Matsugasako, and Y. Takeda Granule-Bound Starch Synthase I is Responsible for Biosynthesis of Extra-Long Unit Chains of Amylopectin in Rice Plant Cell Physiol., June 1, 2008; 49(6): 925 - 933. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Fujita, M. Yoshida, T. Kondo, K. Saito, Y. Utsumi, T. Tokunaga, A. Nishi, H. Satoh, J.-H. Park, J.-L. Jane, et al. Characterization of SSIIIa-Deficient Mutants of Rice: The Function of SSIIIa and Pleiotropic Effects by SSIIIa Deficiency in the Rice Endosperm Plant Physiology, August 1, 2007; 144(4): 2009 - 2023. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Smith, D. C. Fulton, T. Chia, D. Thorneycroft, A. Chapple, H. Dunstan, C. Hylton, S. C. Zeeman, and A. M. Smith Diurnal Changes in the Transcriptome Encoding Enzymes of Starch Metabolism Provide Evidence for Both Transcriptional and Posttranscriptional Regulation of Starch Metabolism in Arabidopsis Leaves Plant Physiology, September 1, 2004; 136(1): 2687 - 2699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Pilling and A. M. Smith Growth Ring Formation in the Starch Granules of Potato Tubers Plant Physiology, May 1, 2003; 132(1): 365 - 371. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Geigenberger Regulation of sucrose to starch conversion in growing potato tubers J. Exp. Bot., January 3, 2003; 54(382): 457 - 465. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Edwards, J.-P. Vincken, L. C. J. M. Suurs, R. G. F. Visser, S. Zeeman, A. Smith, and C. Martin Discrete Forms of Amylose Are Synthesized by Isoforms of GBSSI in Pea PLANT CELL, August 1, 2002; 14(8): 1767 - 1785. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |