Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M110729200 on January 30, 2002

J. Biol. Chem., Vol. 277, Issue 15, 12689-12696, April 12, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/15/12689    most recent
M110729200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ni, H.
Right arrow Articles by Weissman, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ni, H.
Right arrow Articles by Weissman, D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Extracellular mRNA Induces Dendritic Cell Activation by Stimulating Tumor Necrosis Factor-alpha Secretion and Signaling through a Nucleotide Receptor*

Houping NiDagger , John CapodiciDagger , Georgetta CannonDagger , Didier Communi§, Jean-Marie Boeynaems§, Katalin Karikó, and Drew WeissmanDagger ||

From the Divisions of Dagger  Infectious Diseases and  Neurosurgery, University of Pennsylvania, Philadelphia, Pennsylvania 19104 and the § Institute of Interdisciplinary Research and Department of Medical Chemistry, School of Medicine, Erasme Hospital, Université Libre de Bruxelles, Brussels 1070, Belgium

We previously demonstrated that dendritic cell (DC) pulsing with antigen-encoded mRNA resulted in the loading of both major histocompatibility complex class I and II antigen presentation pathways and the delivery of an activation signal. Coculture of mRNA-pulsed DC with T cells led to the induction of a potent primary immune response. DC, in addition to recognizing foreign antigens through pattern recognition receptors, also must respond to altered self, transformed, or intracellularly infected cells. This occurs through cell surface receptors that recognize products of inflammation and cell death. In this report, we characterize two signaling pathways utilized by extracellular mRNA to activate DC. In addition, a novel ligand, poly(A), is identified that mediates signaling through a receptor that can be inhibited by pertussis toxin and suramin and can be desensitized by ATP and ADP, suggesting a P2Y type nucleotide receptor. The role of this signaling activity in vaccine design and the potential effect of mRNA released by damaged cells in the induction of immune responsiveness is discussed.

* This work was supported by NHLBI, National Institutes of Health (NIH) Grant R01-HL-62060-1 and by NIAID, NIH Grant R21-AI-45318-01A1.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|| To whom correspondence should be addressed: Division of Infectious Diseases, University of Pennsylvania, 522B Johnson Pavilion, Philadelphia, PA 19104. Tel.: 215-614-0291; Fax: 215-349-5111; E-mail: dreww@mail.med.upenn.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Lichtnekert, V. Vielhauer, D. Zecher, O. P. Kulkarni, S. Clauss, S. Segerer, V. Hornung, T. N. Mayadas, B. Beutler, S. Akira, et al.
Trif is not required for immune complex glomerulonephritis: dying cells activate mesangial cells via Tlr2/Myd88 rather than Tlr3/Trif
Am J Physiol Renal Physiol, April 1, 2009; 296(4): F867 - F874.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
U. Koedel, U. M. Merbt, C. Schmidt, B. Angele, B. Popp, H. Wagner, H.-W. Pfister, and C. J. Kirschning
Acute Brain Injury Triggers MyD88-Dependent, TLR2/4-Independent Inflammatory Responses
Am. J. Pathol., July 1, 2007; 171(1): 200 - 213.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
F. Marteau, D. Communi, J.-M. Boeynaems, and N. Suarez Gonzalez
Involvement of multiple P2Y receptors and signaling pathways in the action of adenine nucleotides diphosphates on human monocyte-derived dendritic cells
J. Leukoc. Biol., October 1, 2004; 76(4): 796 - 803.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Kariko, H. Ni, J. Capodici, M. Lamphier, and D. Weissman
mRNA Is an Endogenous Ligand for Toll-like Receptor 3
J. Biol. Chem., March 26, 2004; 279(13): 12542 - 12550.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
F. Marteau, E. Le Poul, D. Communi, D. Communi, C. Labouret, P. Savi, J.-M. Boeynaems, and N. S. Gonzalez
Pharmacological Characterization of the Human P2Y13 Receptor
Mol. Pharmacol., July 1, 2003; 64(1): 104 - 112.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
Z. Su, J. Dannull, A. Heiser, D. Yancey, S. Pruitt, J. Madden, D. Coleman, D. Niedzwiecki, E. Gilboa, and J. Vieweg
Immunological and Clinical Responses in Metastatic Renal Cancer Patients Vaccinated with Tumor RNA-transfected Dendritic Cells
Cancer Res., May 1, 2003; 63(9): 2127 - 2133.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement