JBC PeproTech; Our Business is Cytokines!

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M110795200 on January 25, 2002

J. Biol. Chem., Vol. 277, Issue 15, 12931-12936, April 12, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/15/12931    most recent
M110795200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kira, M.
Right arrow Articles by Itami, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kira, M.
Right arrow Articles by Itami, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

STAT3 Deficiency in Keratinocytes Leads to Compromised Cell Migration through Hyperphosphorylation of p130cas*

Masahiro KiraDagger , Shigetoshi SanoDagger , Satoshi TakagiDagger , Kunihiko YoshikawaDagger , Junji Takeda§, and Satoshi ItamiDagger

From the Dagger  Department of Dermatology, Course of Molecular Medicine, and the § Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan

We previously reported that STAT3 plays a crucial role in transducing a signal for migration of keratinocytes (Sano, S., Itami, S., Takeda, K., Tarutani, M., Yamaguchi, Y., Miura, H., Yoshikawa, K., Akira, S., and Takeda, J. (1999) EMBO J. 18, 4657-4668). To clarify the role of STAT3 in signaling the migration, we studied the intracellular signaling pathway through an integrin receptor in STAT3-deficient keratinocytes. STAT3-deficient keratinocytes demonstrated increased adhesiveness and fast spreading on a collagen matrix. Staining with anti-phosphotyrosine antibody revealed that STAT3-deficient keratinocytes had an increased number of tyrosyl-hyperphosphorylated focal adhesions. Analyses with immunoprecipitation revealed that p130cas was constitutively hyperphosphorylated on tyrosine residues, while other focal adhesion molecules such as focal adhesion kinase and paxillin were not. Transfection of STAT3-deficient keratinocytes with an adenoviral vector encoding the wild-type Stat3 gene reversed not only impaired migration but also the increased tyrosine phosphorylation of p130cas. These results strongly suggest that STAT3 in keratinocytes plays a critical role in turnover of tyrosine phosphorylation of p130cas, modulating cell adhesiveness to the substratum leading to growth factor-dependent cell migration.

* This work was supported by grants from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Dept. of Dermatology, Course of Molecular Medicine, Graduate School of Medicine, Osaka University, 2-2, Yamadaoka, Suita 565-0871, Japan. Tel.: 81-6-6879-3031; Fax: 81-6-6879-3039; E-mail: itami@derma.med.osaka-u.ac.jp.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
G. Z. Cheng, W. Zhang, M. Sun, Q. Wang, D. Coppola, M. Mansour, L. Xu, C. Costanzo, J. Q. Cheng, and L.-H. Wang
Twist Is Transcriptionally Induced by Activation of STAT3 and Mediates STAT3 Oncogenic Function
J. Biol. Chem., May 23, 2008; 283(21): 14665 - 14673.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
D. Germain and D. A. Frank
Targeting the Cytoplasmic and Nuclear Functions of Signal Transducers and Activators of Transcription 3 for Cancer Therapy
Clin. Cancer Res., October 1, 2007; 13(19): 5665 - 5669.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Azare, K. Leslie, H. Al-Ahmadie, W. Gerald, P. H. Weinreb, S. M. Violette, and J. Bromberg
Constitutively Activated Stat3 Induces Tumorigenesis and Enhances Cell Motility of Prostate Epithelial Cells through Integrin {beta}6
Mol. Cell. Biol., June 15, 2007; 27(12): 4444 - 4453.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
A J Craven, A J Nixon, M G Ashby, C J Ormandy, K Blazek, R J Wilkins, and A J Pearson
Prolactin delays hair regrowth in mice.
J. Endocrinol., November 1, 2006; 191(2): 415 - 425.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
S. P. Gao and J. F. Bromberg
Touched and Moved by STAT3
Sci. Signal., July 11, 2006; 2006(343): pe30 - pe30.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. M. McLoughlin, B. J. Jenkins, D. Grail, A. S. Williams, C. A. Fielding, C. R. Parker, M. Ernst, N. Topley, and S. A. Jones
IL-6 trans-signaling via STAT3 directs T cell infiltration in acute inflammation
PNAS, July 5, 2005; 102(27): 9589 - 9594.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. Sano, K. S. Chan, M. Kira, K. Kataoka, S. Takagi, M. Tarutani, S. Itami, K. Kiguchi, M. Yokoi, K. Sugasawa, et al.
Signal Transducer and Activator of Transcription 3 Is a Key Regulator of Keratinocyte Survival and Proliferation following UV Irradiation
Cancer Res., July 1, 2005; 65(13): 5720 - 5729.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Debidda, L. Wang, H. Zang, V. Poli, and Y. Zheng
A Role of STAT3 in Rho GTPase-regulated Cell Migration and Proliferation
J. Biol. Chem., April 29, 2005; 280(17): 17275 - 17285.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Boniface, F.-X. Bernard, M. Garcia, A. L. Gurney, J.-C. Lecron, and F. Morel
IL-22 Inhibits Epidermal Differentiation and Induces Proinflammatory Gene Expression and Migration of Human Keratinocytes
J. Immunol., March 15, 2005; 174(6): 3695 - 3702.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Z.-l. Yuan, Y.-j. Guan, L. Wang, W. Wei, A. B. Kane, and Y. E. Chin
Central Role of the Threonine Residue within the p+1 Loop of Receptor Tyrosine Kinase in STAT3 Constitutive Phosphorylation in Metastatic Cancer Cells
Mol. Cell. Biol., November 1, 2004; 24(21): 9390 - 9400.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
C. Weidt, B. Niggemann, W. Hatzmann, K. S. Zanker, and T. Dittmar
Differential Effects of Culture Conditions on the Migration Pattern of Stromal Cell-Derived Factor-Stimulated Hematopoietic Stem Cells
Stem Cells, November 1, 2004; 22(6): 890 - 896.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Armulik, T. Velling, and S. Johansson
The Integrin {beta}1 Subunit Transmembrane Domain Regulates Phosphatidylinositol 3-Kinase-dependent Tyrosine Phosphorylation of Crk-associated Substrate
Mol. Biol. Cell, June 1, 2004; 15(6): 2558 - 2567.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Cui, L. Huang, F. Elefteriou, G. Yang, J. M. Shelton, J. E. Giles, O. K. Oz, T. Pourbahrami, C. Y. H. Lu, J. A. Richardson, et al.
Essential Role of STAT3 in Body Weight and Glucose Homeostasis
Mol. Cell. Biol., January 1, 2004; 24(1): 258 - 269.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.