JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M111661200 on January 30, 2002

J. Biol. Chem., Vol. 277, Issue 15, 13138-13147, April 12, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/15/13138    most recent
M111661200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yarwood, J. M.
Right arrow Articles by Schlievert, P. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yarwood, J. M.
Right arrow Articles by Schlievert, P. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Characterization and Expression Analysis of Staphylococcus aureus Pathogenicity Island 3
IMPLICATIONS FOR THE EVOLUTION OF STAPHYLOCOCCAL PATHOGENICITY ISLANDS*

Jeremy M. YarwoodDagger §, John K. McCormickDagger Dagger Dagger , Michael L. Paustian, Paul M. OrwinDagger ||, Vivek Kapur, and Patrick M. SchlievertDagger **

From the Dagger  Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, and  Department of Veterinary Pathobiology and Biomedical Genomics Center, University of Minnesota, St. Paul, Minnesota 55108

We describe the complete sequence of the 15.9-kb staphylococcal pathogenicity island 3 encoding staphylococcal enterotoxin serotypes B, K, and Q. The island, which meets the generally accepted definition of pathogenicity islands, contains 24 open reading frames potentially encoding proteins of more than 50 amino acids, including an apparently functional integrase. The element is bordered by two 17-bp direct repeats identical to those found flanking staphylococcal pathogenicity island 1. The island has extensive regions of homology to previously described pathogenicity islands, particularly staphylococcal pathogenicity islands 1 and bov. The expression of 22 of the 24 open reading frames contained on staphylococcal pathogenicity island 3 was detected either in vitro during growth in a laboratory medium or serum or in vivo in a rabbit model of toxic shock syndrome using DNA microarrays. The effect of oxygen tension on staphylococcal pathogenicity island 3 gene expression was also examined. By comparison with the known staphylococcal pathogenicity islands in the context of gene expression described here, we propose a model of pathogenicity island origin and evolution involving specialized transduction events and addition, deletion, or recombination of pathogenicity island "modules."

* This work was supported in part by National Institutes of Health Grant AI22159 (to P. M. S.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AF410775.

§ Supported by a Howard Hughes Predoctoral Fellowship in the Biological Sciences.

|| Present address: Dept. of Environmental Science and Engineering, California Institute of Technology, 209 Keck Laboratories, M/C 138-78, Pasadena, CA 91125-7800.

** To whom correspondence should be addressed: Dept. of Microbiology, University of Minnesota Medical School, MMC 196, 420 Delaware St. SE, Minneapolis, MN 55455. Tel.: 612-624-9471; Fax: 612-626-0623; E-mail: pats@lenti.med.umn.edu.

Dagger Dagger Present address: The Lawson Health Research Inst., The University of Western Ontario, Grosvenor Campus, 268 Grovenor Street, Rm. H-323, London, Ontario, Canada N6A4V2.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
G. Blaiotta, V. Fusco, C. von Eiff, F. Villani, and K. Becker
Biotyping of Enterotoxigenic Staphylococcus aureus by Enterotoxin Gene Cluster (egc) Polymorphism and spa Typing Analyses
Appl. Envir. Microbiol., September 1, 2006; 72(9): 6117 - 6123.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. Muniesa, M. A. Schembri, N. Hauf, and T. Chakraborty
Active Genetic Elements Present in the Locus of Enterocyte Effacement in Escherichia coli O26 and Their Role in Mobility
Infect. Immun., July 1, 2006; 74(7): 4190 - 4199.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. Omoe, K. Imanishi, D.-L. Hu, H. Kato, Y. Fugane, Y. Abe, S. Hamaoka, Y. Watanabe, A. Nakane, T. Uchiyama, et al.
Characterization of Novel Staphylococcal Enterotoxin-Like Toxin Type P
Infect. Immun., September 1, 2005; 73(9): 5540 - 5546.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
D. S Smyth, P. J Hartigan, W. J Meaney, J R. Fitzgerald, C. F Deobald, G. A Bohach, and C. J Smyth
Superantigen genes encoded by the egc cluster and SaPIbov are predominant among Staphylococcus aureus isolates from cows, goats, sheep, rabbits and poultry
J. Med. Microbiol., April 1, 2005; 54(4): 401 - 411.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
J. M. Fueyo, M. C. Mendoza, M. R. Rodicio, J. Muniz, M. A. Alvarez, and M. C. Martin
Cytotoxin and Pyrogenic Toxin Superantigen Gene Profiles of Staphylococcus aureus Associated with Subclinical Mastitis in Dairy Cows and Relationships with Macrorestriction Genomic Profiles
J. Clin. Microbiol., March 1, 2005; 43(3): 1278 - 1284.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
A. Lovseth, S. Loncarevic, and K. G. Berdal
Modified Multiplex PCR Method for Detection of Pyrogenic Exotoxin Genes in Staphylococcal Isolates
J. Clin. Microbiol., August 1, 2004; 42(8): 3869 - 3872.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. Holtfreter, K. Bauer, D. Thomas, C. Feig, V. Lorenz, K. Roschack, E. Friebe, K. Selleng, S. Lovenich, T. Greve, et al.
egc-Encoded Superantigens from Staphylococcus aureus Are Neutralized by Human Sera Much Less Efficiently than Are Classical Staphylococcal Enterotoxins or Toxic Shock Syndrome Toxin
Infect. Immun., July 1, 2004; 72(7): 4061 - 4071.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. T. G. Holden, E. J. Feil, J. A. Lindsay, S. J. Peacock, N. P. J. Day, M. C. Enright, T. J. Foster, C. E. Moore, L. Hurst, R. Atkin, et al.
Complete genomes of two clinical Staphylococcus aureus strains: Evidence for the rapid evolution of virulence and drug resistance
PNAS, June 29, 2004; 101(26): 9786 - 9791.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. Omoe, K. Imanishi, D.-L. Hu, H. Kato, H. Takahashi-Omoe, A. Nakane, T. Uchiyama, and K. Shinagawa
Biological Properties of Staphylococcal Enterotoxin-Like Toxin Type R
Infect. Immun., June 1, 2004; 72(6): 3664 - 3667.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
N. Sergeev, D. Volokhov, V. Chizhikov, and A. Rasooly
Simultaneous Analysis of Multiple Staphylococcal Enterotoxin Genes by an Oligonucleotide Microarray Assay
J. Clin. Microbiol., May 1, 2004; 42(5): 2134 - 2143.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. Ben Zakour, M. Gautier, R. Andonov, D. Lavenier, M.-F. Cochet, P. Veber, A. Sorokin, and Y. Le Loir
GenoFrag: software to design primers optimized for whole genome scanning by long-range PCR amplification
Nucleic Acids Res., January 2, 2004; 32(1): 17 - 24.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
H. Schmidt and M. Hensel
Pathogenicity Islands in Bacterial Pathogenesis
Clin. Microbiol. Rev., January 1, 2004; 17(1): 14 - 56.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
P. Sumby and M. K. Waldor
Transcription of the Toxin Genes Present within the Staphylococcal Phage {phi}Sa3ms Is Intimately Linked with the Phage's Life Cycle
J. Bacteriol., December 1, 2003; 185(23): 6841 - 6851.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. Omoe, D.-L. Hu, H. Takahashi-Omoe, A. Nakane, and K. Shinagawa
Identification and Characterization of a New Staphylococcal Enterotoxin-Related Putative Toxin Encoded by Two Kinds of Plasmids
Infect. Immun., October 1, 2003; 71(10): 6088 - 6094.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
C. Canchaya, C. Proux, G. Fournous, A. Bruttin, and H. Brussow
Prophage Genomics
Microbiol. Mol. Biol. Rev., June 1, 2003; 67(2): 238 - 276.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
D.-L. Hu, K. Omoe, Y. Shimoda, A. Nakane, and K. Shinagawa
Induction of Emetic Response to Staphylococcal Enterotoxins in the House Musk Shrew (Suncus murinus)
Infect. Immun., January 1, 2003; 71(1): 567 - 570.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. D. Boyce, I. Wilkie, M. Harper, M. L. Paustian, V. Kapur, and B. Adler
Genomic Scale Analysis of Pasteurella multocida Gene Expression during Growth within the Natural Chicken Host
Infect. Immun., December 1, 2002; 70(12): 6871 - 6879.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.