HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 277, Issue 16, 13379-13388, April 19, 2002

This Article Full Text Full Text (PDF) All Versions of this Article: 277/16/13379    most recent M108079200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kirsch, M. Articles by de Groot, H. Search for Related Content PubMed PubMed Citation Articles by Kirsch, M. Articles by de Groot, H. Social Bookmarking                      What's this?

Formation of Peroxynitrite from Reaction of Nitroxyl Anion with Molecular Oxygen^*

Michael Kirsch and Herbert de Groot

From the Institut für Physiologische Chemie, Universitätsklinikum, Hufelandstrasse 55, D-45122 Essen, Germany

Peroxynitrite (ONOO/ONOOH) is generally expected to be formed in vivo from the diffusion-controlled reaction between superoxide (O) and nitric oxide (^·NO). In the present paper we show that under aerobic conditions the nitroxyl anion (NO), released from Angeli's salt (disodium diazen-1-ium-1,2,2-triolate, ON=NO), generated peroxynitrite with a yield of about 65%. Simultaneously, hydroxyl radicals are formed from the nitroxyl anion with a yield of about 3% via a minor, peroxynitrite-independent pathway. Further experiments clearly underline that the chemistry of NO in the presence of oxygen is mainly characterized by peroxynitrite and not by HO^· radicals. Quantum-chemical calculations predict that peroxynitrite formation should proceed via intermediary formation of ^·NO and O, probably by an electron-transfer mechanism. This prediction is supported by the fact that H₂O₂ is formed during the decay of NO in the presence of superoxide dismutase (Cu(II),Zn-SOD). Since the nitroxyl anion may be released endogenously by a variety of biomolecules, substantial amounts of peroxynitrite might be formed in vivo via NO in addition to the "classical" ^·NO + O pathway.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				K. Sonnenschein, H. de Groot, and M. Kirsch Formation of S-Nitrosothiols from Regiospecific Reaction of Thiols with N-Nitrosotryptophan Derivatives J. Biol. Chem., October 29, 2004; 279(44): 45433 - 45440. [Abstract] [Full Text] [PDF]

				J. Ivanova, G. Salama, R. M. Clancy, N. F. Schor, K. D. Nylander, and D. A. Stoyanovsky Formation of Nitroxyl and Hydroxyl Radical in Solutions of Sodium Trioxodinitrate: EFFECTS OF pH AND CYTOTOXICITY J. Biol. Chem., October 31, 2003; 278(44): 42761 - 42768. [Abstract] [Full Text] [PDF]

				M. Kirsch, A. Fuchs, and H. de Groot Regiospecific Nitrosation of N-terminal-blocked Tryptophan Derivatives by N2O3 at Physiological pH J. Biol. Chem., March 28, 2003; 278(14): 11931 - 11936. [Abstract] [Full Text] [PDF]