|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 16, 13518-13527, April 19, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Medical Service, Veterans Affairs Palo Alto Health Care
System and the Department of Medicine, Stanford University School
of Medicine, Palo Alto, California 94304, the ¶ Department of
Medicine, National University of Singapore, Singapore
119074, the PEG1 (or MEST) is an
imprinted gene located on human chromosome 7q32 that is expressed
predominantly from the paternal allele. In the mouse,
Peg1/Mest is associated with embryonic growth
and maternal behavior. Human PEG1 is transcribed from two
promoters; the transcript from promoter P1 is derived from both
parental alleles, and the transcript from P2 is exclusively from the
paternal allele. We characterized the P1 and P2 transcripts in various normal and neoplastic tissues. In the normal tissues, PEG1
was transcribed from both promoters P1 and P2, whereas in six of eight neoplastic tissues, PEG1 was transcribed exclusively from
promoter P1. Bisulfite sequencing demonstrated high levels of CpG
methylation in the P2 region of DNA from a lung tumor. In the region
between P1 and P2, we identified a novel transcript,
PEG1-AS, in an antisense orientation to
PEG1. PEG1-AS is a spliced transcript and was
detected as a strong 2.4-kilobase band on a Northern blot.
PEG1-AS and PEG1 P2-sense
transcript were expressed exclusively from the paternal allele.
Fragments of DNA from within the 1.5-kilobase region between PEG1-AS and the P2 exon were ligated to a pGL3
luciferase reporter vector and transfected into NCI H23 cells. This DNA
exhibited strong promoter activity in both the sense and antisense
directions, indicating that PEG1-AS and P2 exon share a
common promoter region. Treatment of the transfected DNA fragments with
CpG methylase abolished the promoter activity. Of interest,
PEG1-AS was expressed predominantly in testis
and in mature motile spermatozoa, indicating a possible role for this
transcript in human sperm physiology and fertilization.
An Imprinted PEG1/MEST Antisense
Expressed Predominantly in Human Testis and in Mature Spermatozoa*
,§,
, Department of Food and Nutrition, Japan Women's
University, Tokyo 113, Japan, and the ** Departments of
Radiation Oncology and Pathology, Duke University Medical Center,
Durham, North Carolina 27710
*
This study was supported by National Institutes of Health
Grant DK36054 and by the Research Service of the Department of Veterans Affairs.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
These authors contributed equally to this work.
§
To whom correspondence may be addressed: Bldg. 101, Rm. B2-125,
Veterans Affairs Palo Alto Health Care System, 3801 Miranda Ave. Palo
Alto, CA 94304. Tel.: 650-493-5000 (ext. 63185); Fax: 650-856-8024; E-mail: thanhvu@stanford.edu or
arhoffman{at}stanford.edu.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  P. P. Luedi, F. S. Dietrich, J. R. Weidman, J. M. Bosko, R. L. Jirtle, and A. J. Hartemink Computational and experimental identification of novel human imprinted genes Genome Res., December 1, 2007; 17(12): 1723 - 1730. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. V. Prasanth and D. L. Spector Eukaryotic regulatory RNAs: an answer to the 'genome complexity' conundrum Genes & Dev., January 1, 2007; 21(1): 11 - 42. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Murphy, Z. Huang, Y. Wen, M. A. Spillman, R. S. Whitaker, L. R. Simel, T. D. Nichols, J. R. Marks, and A. Berchuck Frequent IGF2/H19 Domain Epigenetic Alterations and Elevated IGF2 Expression in Epithelial Ovarian Cancer Mol. Cancer Res., April 1, 2006; 4(4): 283 - 292. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Li, T. H. Vu, G. A. Ulaner, E. Littman, J.-Q. Ling, H.-L. Chen, J.-F. Hu, B. Behr, L. Giudice, and A. R. Hoffman IVF results in de novo DNA methylation and histone methylation at an Igf2-H19 imprinting epigenetic switch Mol. Hum. Reprod., September 1, 2005; 11(9): 631 - 640. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yang, T. Li, T. H. Vu, G. A. Ulaner, J.-F. Hu, and A. R. Hoffman The Histone Code Regulating Expression of the Imprinted Mouse Igf2r Gene Endocrinology, December 1, 2003; 144(12): 5658 - 5670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kiyosawa, I. Yamanaka, N. Osato, S. Kondo, and Y. Hayashizaki Antisense Transcripts With FANTOM2 Clone Set and Their Implications for Gene Regulation Genome Res., June 1, 2003; 13(6): 1324 - 1334. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. H. Vu, N. V. Chuyen, T. Li, and A. R. Hoffman Loss of Imprinting of IGF2 Sense and Antisense Transcripts in Wilms' Tumor Cancer Res., April 15, 2003; 63(8): 1900 - 1905. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L Bentley, K Nakabayashi, D Monk, C Beechey, J Peters, Z Birjandi, F E Khayat, M Patel, M A Preece, P Stanier, et al. The imprinted region on human chromosome 7q32 extends to the carboxypeptidase A gene cluster: an imprinted candidate for Silver-Russell syndrome J. Med. Genet., April 1, 2003; 40(4): 249 - 256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. A. Ulaner, T. H. Vu, T. Li, J.-F. Hu, X.-M. Yao, Y. Yang, R. Gorlick, P. Meyers, J. Healey, M. Ladanyi, et al. Loss of imprinting of IGF2 and H19 in osteosarcoma is accompanied by reciprocal methylation changes of a CTCF-binding site Hum. Mol. Genet., March 1, 2003; 12(5): 535 - 549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nakabayashi, L. Bentley, M. P. Hitchins, K. Mitsuya, M. Meguro, S. Minagawa, J. S. Bamforth, P. Stanier, M. Preece, R. Weksberg, et al. Identification and characterization of an imprinted antisense RNA (MESTIT1) in the human MEST locus on chromosome 7q32 Hum. Mol. Genet., July 15, 2002; 11(15): 1743 - 1756. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |