|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 16, 13628-13634, April 19, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Division of Molecular Physiology, Medical Sciences
Institute/Wellcome Trust Biocentre Complex, Dow Street, University of
Dundee, Dundee DD1 5EH, United Kingdom
SAT1-3 comprise members of the recently cloned
family of System A transporters that mediate the sodium-coupled uptake
of short chain neutral amino acids, and their activity is regulated
extensively by stimuli such as insulin, growth factors, and amino acid
availability. In skeletal muscle, insulin stimulates System A activity
rapidly by a presently ill-defined mechanism. Here we demonstrate that insulin induces an increase in the plasma membrane abundance of SAT2 in
a phosphatidylinositol 3-kinase-dependent manner
and that this increase is derived from an endosomal compartment that is required for the hormonal activation of System A. Chloroquine, an
acidotropic weak base that impairs endosomal recycling of membrane proteins, induced a complete inhibition in the insulin-mediated stimulation of System A, which was associated with a loss in SAT2 recruitment to the plasma membrane. The failure to stimulate System A
and recruit SAT2 to the cell surface could not be attributed to a block
in insulin signaling, as chloroquine had no effect on the
insulin-mediated phosphorylation of protein kinase B or glycogen
synthase kinase 3 or upon insulin-stimulated GLUT4 translocation and
glucose transport. Our data indicate strongly that insulin increases
System A transport in L6 cells by stimulating the exocytosis of SAT2
carriers from a chloroquine-sensitive endosomal compartment.
Insulin Promotes the Cell Surface Recruitment of the SAT2/ATA2
System A Amino Acid Transporter from an Endosomal Compartment in
Skeletal Muscle Cells*
§,, and
*
This work was supported by grants from the Biotechnology and
Biological Sciences Research Council (BBSRC), Medical Research Council,
Diabetes UK, and The Wellcome Trust.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
These authors contributed equally to the work reported.
§
Recipient of a BBSRC-SmithKline Beecham CASE (Co-Operative Awards
in Science and Engineering) studentship.
¶
To whom correspondence should be addressed. Tel.:
44-1382-344969; Fax: 44-1382-345507; E-mail:
h.s.hundal@dundee.ac.uk.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Desforges, K. J. Mynett, R. L. Jones, S. L. Greenwood, M. Westwood, C. P. Sibley, and J. D. Glazier The SNAT4 isoform of the system A amino acid transporter is functional in human placental microvillous plasma membrane J. Physiol., January 1, 2009; 587(1): 61 - 72. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Green, O. Goransson, G. S. Kular, N. R. Leslie, A. Gray, D. R. Alessi, K. Sakamoto, and H. S. Hundal Use of Akt Inhibitor and a Drug-resistant Mutant Validates a Critical Role for Protein Kinase B/Akt in the Insulin-dependent Regulation of Glucose and System A Amino Acid Uptake J. Biol. Chem., October 10, 2008; 283(41): 27653 - 27667. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Hyde, E. L. Cwiklinski, K. MacAulay, P. M. Taylor, and H. S. Hundal Distinct Sensor Pathways in the Hierarchical Control of SNAT2, a Putative Amino Acid Transceptor, by Amino Acid Availability J. Biol. Chem., July 6, 2007; 282(27): 19788 - 19798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hatanaka, Y. Hatanaka, J.-i. Tsuchida, V. Ganapathy, and M. Setou Amino Acid Transporter ATA2 Is Stored at the trans-Golgi Network and Released by Insulin Stimulus in Adipocytes J. Biol. Chem., December 22, 2006; 281(51): 39273 - 39284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hatanaka, Y. Hatanaka, and M. Setou Regulation of Amino Acid Transporter ATA2 by Ubiquitin Ligase Nedd4-2 J. Biol. Chem., November 24, 2006; 281(47): 35922 - 35930. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Lopez, N. Torres, V. Ortiz, G. Aleman, R. Hernandez-Pando, and A. R. Tovar Characterization and regulation of the gene expression of amino acid transport system A (SNAT2) in rat mammary gland Am J Physiol Endocrinol Metab, November 1, 2006; 291(5): E1059 - E1066. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Schmelzle, S. Kane, S. Gridley, G. E. Lienhard, and F. M. White Temporal dynamics of tyrosine phosphorylation in insulin signaling. Diabetes, August 1, 2006; 55(8): 2171 - 2179. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H N Jones, C J Ashworth, K R Page, and H J McArdle Expression and adaptive regulation of amino acid transport system A in a placental cell line under amino acid restriction. Reproduction, May 1, 2006; 131(5): 951 - 960. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gu, C. J. Villegas, and J. X. Jiang Differential Regulation of Amino Acid Transporter SNAT3 by Insulin in Hepatocytes J. Biol. Chem., July 15, 2005; 280(28): 26055 - 26062. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. G. Giresi, E. J. Stevenson, J. Theilhaber, A. Koncarevic, J. Parkington, R. A. Fielding, and S. C. Kandarian Identification of a molecular signature of sarcopenia Physiol Genomics, April 14, 2005; 21(2): 253 - 263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhao, R. Hyde, and H. S Hundal Signalling mechanisms underlying the rapid and additive stimulation of NKCC activity by insulin and hypertonicity in rat L6 skeletal muscle cells J. Physiol., October 1, 2004; 560(1): 123 - 136. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Burkhalter, H. Fiumelli, I. Allaman, J.-Y. Chatton, and J.-L. Martin Brain-Derived Neurotrophic Factor Stimulates Energy Metabolism in Developing Cortical Neurons J. Neurosci., September 10, 2003; 23(23): 8212 - 8220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Mackenzie, M. K.-H. Schafer, J. D. Erickson, M. A. Hediger, E. Weihe, and H. Varoqui Functional Properties and Cellular Distribution of the System A Glutamine Transporter SNAT1 Support Specialized Roles in Central Neurons J. Biol. Chem., June 20, 2003; 278(26): 23720 - 23730. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. T. Brosnan Interorgan Amino Acid Transport and its Regulation J. Nutr., June 1, 2003; 133(6): 2068S - 2072. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |