JBC Avanti Polar Lipids

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M107811200 on January 30, 2002

J. Biol. Chem., Vol. 277, Issue 16, 13952-13958, April 19, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/16/13952    most recent
M107811200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Razmara, M.
Right arrow Articles by Alnemri, E. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Razmara, M.
Right arrow Articles by Alnemri, E. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

CARD-8 Protein, a New CARD Family Member That Regulates Caspase-1 Activation and Apoptosis*

Marjaneh RazmaraDagger , Srinivasa M. SrinivasulaDagger §, Lin Wang, Jean-Luc PoyetDagger , Brad J. Geddes, Peter S. DiStefano, John Bertin||, and Emad S. AlnemriDagger **

From the Dagger  Center for Apoptosis Research and the Department of Microbiology and Immunology, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107 and  Millennium Pharmaceuticals, Inc., Cambridge, Massachusetts 02139

Caspase-associated recruitment domains (CARD) are protein-protein interaction modules found extensively in proteins that play important roles in apoptosis, NFkappa B activation, and cytokine regulation. In this study we identified a novel human protein, CARD-8, which contains a C-terminal CARD domain with high similarity to the CARD domain of caspase-1/ICE. We demonstrate that CARD-8 interacts physically with caspase-1 and negatively regulates caspase-1-dependent IL-1beta generation in the THP-1 monocytic cell line. CARD-8 binds also to ICEBERG and pseudo-ICE, two other recently identified proteins, which bind to the CARD domain of caspase-1 and negatively regulate its activity. Reverse transcriptase-PCR analysis revealed that CARD-8 is expressed mainly in monocytes, placenta, lymph nodes, and spleen. This pattern of expression is consistent with caspase-1 expression in the same cells and tissues. CARD-8 was also found to negatively regulate NF-kappa B activation by TNF-alpha stimulation and by ectopically expressed RICK, suggesting that this protein may control cell survival. Consistent with these results, stable expression of CARD-8 in U937 or THP-1 cells sensitizes the cells to differentiation-induced apoptosis. Overexpression of CARD-8 can also induce apoptosis in transfected cells. The results suggest that CARD-8 represents a new signaling molecule involved in the regulation of caspase-1 and NF-kappa B activation.

* This work was supported by National Institutes of Health Grants CA85421 and AG14357 (to E. S. A.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AF322184.

§ Special Fellow of the Leukemia and Lymphoma Society.

** To whom correspondence may be addressed: Thomas Jefferson University, Kimmel Cancer Inst., Bluemle Life Sciences Bldg., Rm. 904, 233 S. 10th St., Philadelphia, PA 19107. Tel.: 215-503-4632; Fax: 215-923-1098; E-mail: E_Alnemri@lac.jci.tju.edu.

|| To whom correspondence may be addressed: Millennium Pharmaceuticals, Inc., 640 Memorial Dr., Cambridge, MA 02139. Tel.: 617-679-7215; Fax: 617-679-7071; E-mail: bertin@mpi.com.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
J. M. Wilmanski, T. Petnicki-Ocwieja, and K. S. Kobayashi
NLR proteins: integral members of innate immunity and mediators of inflammatory diseases
J. Leukoc. Biol., January 1, 2008; 83(1): 13 - 30.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Fontalba, V. Martinez-Taboada, O. Gutierrez, C. Pipaon, N. Benito, A. Balsa, R. Blanco, and J. L. Fernandez-Luna
Deficiency of the NF-{kappa}B Inhibitor Caspase Activating and Recruitment Domain 8 in Patients with Rheumatoid Arthritis Is Associated with Disease Severity
J. Immunol., October 1, 2007; 179(7): 4867 - 4873.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. M. Kahlenberg, K. C. Lundberg, S. B. Kertesy, Y. Qu, and G. R. Dubyak
Potentiation of Caspase-1 Activation by the P2X7 Receptor Is Dependent on TLR Signals and Requires NF-{kappa}B-Driven Protein Synthesis
J. Immunol., December 1, 2005; 175(11): 7611 - 7622.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. Yamamoto, T. Torigoe, K. Kamiguchi, Y. Hirohashi, K. Nakanishi, C. Nabeta, H. Asanuma, T. Tsuruma, T. Sato, F. Hata, et al.
A Novel Isoform of TUCAN Is Overexpressed in Human Cancer Tissues and Suppresses Both Caspase-8- and Caspase-9-Mediated Apoptosis
Cancer Res., October 1, 2005; 65(19): 8706 - 8714.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Kinoshita, Y. Wang, M. Hasegawa, R. Imamura, and T. Suda
PYPAF3, a PYRIN-containing APAF-1-like Protein, Is a Feedback Regulator of Caspase-1-dependent Interleukin-1{beta} Secretion
J. Biol. Chem., June 10, 2005; 280(23): 21720 - 21725.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. E. Massa, X. Li, A. Hanidu, J. Siamas, M. Pariali, J. Pareja, A. G. Savitt, K. M. Catron, J. Li, and K. B. Marcu
Gene Expression Profiling in Conjunction with Physiological Rescues of IKK{alpha}-null Cells with Wild Type or Mutant IKK{alpha} Reveals Distinct Classes of IKK{alpha}/NF-{kappa}B-dependent Genes
J. Biol. Chem., April 8, 2005; 280(14): 14057 - 14069.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Lamkanfi, G. Denecker, M. Kalai, K. D'hondt, A. Meeus, W. Declercq, X. Saelens, and P. Vandenabeele
INCA, a Novel Human Caspase Recruitment Domain Protein That Inhibits Interleukin-1{beta} Generation
J. Biol. Chem., December 10, 2004; 279(50): 51729 - 51738.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Stehlik, H. Hayashi, F. Pio, A. Godzik, and J. C. Reed
CARD6 Is a Modulator of NF-{kappa}B Activation by Nod1- and Cardiak-mediated Pathways
J. Biol. Chem., August 22, 2003; 278(34): 31941 - 31949.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. M. Srinivasula, J.-L. Poyet, M. Razmara, P. Datta, Z. Zhang, and E. S. Alnemri
The PYRIN-CARD Protein ASC Is an Activating Adaptor for Caspase-1
J. Biol. Chem., June 7, 2002; 277(24): 21119 - 21122.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.