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Originally published In Press as doi:10.1074/jbc.M112053200 on February 4, 2002

J. Biol. Chem., Vol. 277, Issue 16, 14031-14039, April 19, 2002
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Lack of the Small Plastid-encoded PsbJ Polypeptide Results in a Defective Water-splitting Apparatus of Photosystem II, Reduced Photosystem I Levels, and Hypersensitivity to Light*

Martin HagerDagger , Marita HermannDagger , Klaus BiehlerDagger , Anja Krieger-Liszkay§, and Ralph BockDagger ||

From the Dagger  Institut für Biologie III, Universität Freiburg, Schänzlestraße 1, D-79104 Freiburg, Germany, the § Institut für Biologie II, Universität Freiburg, Schänzlestraße 1, D-79104 Freiburg, Germany, and the  Westfälische Wilhelms-Universität Münster, Institut für Biochemie und Biotechnologie der Pflanzen, Hindenburgplatz 55, D-48143 Münster, Germany

Photosystem II is a large pigment-protein complex catalyzing water oxidation and initiating electron transfer processes across the thylakoid membrane. In addition to large protein subunits, many of which bind redox cofactors, photosystem II particles contain a number of low molecular weight polypeptides whose function is only poorly defined. Here we have investigated the function of one of the smallest polypeptides in photosystem II, PsbJ. Using a reverse genetics approach, we have inactivated the psbJ gene in the tobacco chloroplast genome. We show that, although the PsbJ polypeptide is not principally required for functional photosynthetic electron transport, plants lacking PsbJ are unable to grow photoautotrophically. We provide evidence that this is due to the accumulation of incompletely assembled water-splitting complexes, which in turn causes drastically reduced photosynthetic performance and extreme hypersensitivity to light. Our results suggest a role of PsbJ for the stable assembly of the water-splitting complex of photosystem II and, in addition, support a control of photosystem I accumulation through photosystem II activity.

* This work was supported by Deutsche Forschungsgemeinschaft Grant BO 1482/1-4, a grant from Graduiertenkolleg "Molekulare Mechanismen pflanzlicher Differenzierung"), and a grant from the State of Baden-Württemberg (Landesforschungsschwerpunkt) (to R. B.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|| To whom correspondence should be addressed. Tel.: 49-251-8324790; Fax: 49-251-8328371; E-mail: rbock@uni-muenster.de.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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