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J. Biol. Chem., Vol. 277, Issue 17, 14509-14513, April 26, 2002
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From the Laboratory of Biochemistry, Institute of Developmental
Biology, Vavilova Street 26, 117808 Moscow, Russia
A cell-free system derived from
Drosophila embryos was used to investigate positioning of
nucleosomes on specific DNA sequences. This system can be used
to reconstitute differently acetylated nucleosome arrays possessing
ATP-dependent dynamic properties that are not observed with
chromatin assembled from pure components. Nucleosome positioning on
different DNA sequences was studied by restriction endonuclease assay.
The sequence of DNA and the acetylation status of histones had
profound effects on the distribution of nucleosomes, suggesting their
cooperative effect on nucleosome repositioning.
To whom correspondence should be addressed. Tel.: 7-095-135-8847;
Fax: 7-095-135-8012; E-mail: wkrajewski@hotmail.com.
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