JBC Ideal method for primary cell transfection

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Originally published In Press as doi:10.1074/jbc.M107510200 on February 21, 2002

J. Biol. Chem., Vol. 277, Issue 17, 14509-14513, April 26, 2002
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Histone Acetylation Status and DNA Sequence Modulate ATP-dependent Nucleosome Repositioning*

Wladyslaw A. KrajewskiDagger

From the Laboratory of Biochemistry, Institute of Developmental Biology, Vavilova Street 26, 117808 Moscow, Russia

A cell-free system derived from Drosophila embryos was used to investigate positioning of nucleosomes on specific DNA sequences. This system can be used to reconstitute differently acetylated nucleosome arrays possessing ATP-dependent dynamic properties that are not observed with chromatin assembled from pure components. Nucleosome positioning on different DNA sequences was studied by restriction endonuclease assay. The sequence of DNA and the acetylation status of histones had profound effects on the distribution of nucleosomes, suggesting their cooperative effect on nucleosome repositioning.


* This work was supported by the Russian Foundation for Basic Research.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed. Tel.: 7-095-135-8847; Fax: 7-095-135-8012; E-mail: wkrajewski@hotmail.com.


Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.


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