![]()
|
|
||||||||
J. Biol. Chem., Vol. 277, Issue 18, 15677-15689, May 3, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the a Unité d'Immunologie Virale,
d Unité de Chimie Organique, e Unité de
Défense Innée et Inflammation/INSERM U485 de l'Institut
Pasteur, 28 Rue du Dr. Roux, 75724 Paris Cedex 15, g INRA,
Station de Pathologie Végétale, BP81 33883 Villeneuve
d'Ornon, and h Unité de Neurovirologie et
Régénération du Système Nerveux de l'Institut
Pasteur, 25 Rue du Dr. Roux, 75724 Paris Cedex 15, France
Activation of CXCR4 by the CXC chemokine stromal
cell-derived factor-1 (SDF-1) requires interaction of the
amino-terminal domains of both molecules. We report that proteinases
released from either mononucleated blood cells or polymorphonuclear
neutrophils degranulated by inflammatory stimuli generate an SDF-1
fragment that is deleted from amino-terminal residues
Lys1-Pro2-Val3, as
characterized by mass spectrometry analysis. The proteolyzed chemokine fails to induce agonistic functions and is unable to prevent
the fusogenic capacity of CXCR4-tropic human immunodeficiency viruses. Furthermore, we observed that exposure of CXCR4-expressing cells to leukocyte proteinases results in the proteolysis of the extracellular amino-terminal domain of the receptor, as assessed by
flow cytometry analysis and electrophoretic separation of
immunoprecipitated CXCR4. Blockade of SDF-1 and CXCR4 proteolysis by
the specific leukocyte elastase inhibitor,
N-methoxysuccinyl-alanine-alanine-proline-valine-chloromethyl ketone, identified elastase as the major enzyme among
leukocyte-secreted proteinases that accounts for inactivation of both
SDF-1 and CXCR4. Indeed, purified leukocyte elastase generated in
either SDF-1 or CXCR4 a pattern of cleavage indistinguishable from that
observed with leukocyte-secreted proteinases. Our findings suggest that elastase-mediated proteolysis of SDF-1/CXCR4 is part of a mechanism regulating their biological functions in both homeostatic and pathologic processes.
Leukocyte Elastase Negatively Regulates Stromal Cell-derived
Factor-1 (SDF-1)/CXCR4 Binding and Functions by Amino-terminal
Processing of SDF-1 and CXCR4*
*
This work was supported in part by grants from ANRS
(France) and Ensemble Contre le SIDA "SIDACTION" (France).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
This article has been cited by other articles:
![]() |
L. Marquez-Curtis, A. Jalili, K. Deiteren, N. Shirvaikar, A.-M. Lambeir, and A. Janowska-Wieczorek Carboxypeptidase M Expressed by Human Bone Marrow Cells Cleaves the C-Terminal Lysine of Stromal Cell-Derived Factor-1{alpha}: Another Player in Hematopoietic Stem/Progenitor Cell Mobilization? Stem Cells, May 1, 2008; 26(5): 1211 - 1220. [Abstract] [Full Text] [PDF] |
||||
![]() |
C.-H. Wang, W.-J. Cherng, N.-I Yang, C.-M. Hsu, C.-H. Yeh, Y.-J. Lan, J.-S. Wang, and S. Verma Cyclosporine increases ischemia-induced endothelial progenitor cell mobilization through manipulation of the CD26 system Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2008; 294(3): R811 - R818. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. L. Bristow, R. Wolkowicz, M. Trucy, A. Franklin, F. Di Meo, M. T. Kozlowski, R. Winston, and R. R. Arnold NF-{kappa}B Signaling, Elastase Localization, and Phagocytosis Differ in HIV-1 Permissive and Nonpermissive U937 Clones J. Immunol., January 1, 2008; 180(1): 492 - 499. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. MENDEZ-FERRER and P. S. FRENETTE Hematopoietic Stem Cell Trafficking: Regulated Adhesion and Attraction to Bone Marrow Microenvironment Ann. N.Y. Acad. Sci., November 1, 2007; 1116(1): 392 - 413. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. De La Luz Sierra, P. Gasperini, P. J. McCormick, J. Zhu, and G. Tosato Transcription factor Gfi-1 induced by G-CSF is a negative regulator of CXCR4 in myeloid cells Blood, October 1, 2007; 110(7): 2276 - 2285. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Xu, J. Sui, H. Tao, Q. Zhu, and W. A. Marasco Human Anti-CXCR4 Antibodies Undergo VH Replacement, Exhibit Functional V-Region Sulfation, and Define CXCR4 Antigenic Heterogeneity J. Immunol., August 15, 2007; 179(4): 2408 - 2418. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Donini, S. Fontana, G. Savoldi, W. Vermi, L. Tassone, F. Gentili, E. Zenaro, D. Ferrari, L. D. Notarangelo, F. Porta, et al. G-CSF treatment of severe congenital neutropenia reverses neutropenia but does not correct the underlying functional deficiency of the neutrophil in defending against microorganisms Blood, June 1, 2007; 109(11): 4716 - 4723. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Guyon and J.-L. Nahon Multiple actions of the chemokine stromal cell-derived factor-1{alpha} on neuronal activity J. Mol. Endocrinol., March 1, 2007; 38(3): 365 - 376. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Nakayama, N. Mutsuga, and G. Tosato FGF2 posttranscriptionally down-regulates expression of SDF1 in bone marrow stromal cells through FGFR1 IIIc Blood, February 15, 2007; 109(4): 1363 - 1372. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. Vergote, G. S. Butler, M. Ooms, J. H. Cox, C. Silva, M. D. Hollenberg, J. H. Jhamandas, C. M. Overall, and C. Power Proteolytic processing of SDF-1{alpha} reveals a change in receptor specificity mediating HIV-associated neurodegeneration PNAS, December 12, 2006; 103(50): 19182 - 19187. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Kalinkovich, S. Tavor, A. Avigdor, J. Kahn, A. Brill, I. Petit, P. Goichberg, M. Tesio, N. Netzer, E. Naparstek, et al. Functional CXCR4-Expressing Microparticles and SDF-1 Correlate with Circulating Acute Myelogenous Leukemia Cells. Cancer Res., November 15, 2006; 66(22): 11013 - 11020. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. K. Kim, M. De La Luz Sierra, C. K. Williams, A. V. Gulino, and G. Tosato G-CSF down-regulation of CXCR4 expression identified as a mechanism for mobilization of myeloid cells Blood, August 1, 2006; 108(3): 812 - 820. [Abstract] [Full Text] [PDF] |
||||
![]() |
E. Sbaa, J. DeWever, P. Martinive, C. Bouzin, F. Frerart, J.-L. Balligand, C. Dessy, and O. Feron Caveolin Plays a Central Role in Endothelial Progenitor Cell Mobilization and Homing in SDF-1-Driven Postischemic Vasculogenesis Circ. Res., May 12, 2006; 98(9): 1219 - 1227. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Foudi, P. Jarrier, Y. Zhang, M. Wittner, J.-F. Geay, Y. Lecluse, T. Nagasawa, W. Vainchenker, and F. Louache Reduced retention of radioprotective hematopoietic cells within the bone marrow microenvironment in CXCR4-/- chimeric mice Blood, March 15, 2006; 107(6): 2243 - 2251. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Garrood, L. Lee, and C. Pitzalis Molecular mechanisms of cell recruitment to inflammatory sites: general and tissue-specific pathways Rheumatology, March 1, 2006; 45(3): 250 - 260. [Full Text] [PDF] |
||||
![]() |
K. Balabanian, B. Lagane, S. Infantino, K. Y. C. Chow, J. Harriague, B. Moepps, F. Arenzana-Seisdedos, M. Thelen, and F. Bachelerie The Chemokine SDF-1/CXCL12 Binds to and Signals through the Orphan Receptor RDC1 in T Lymphocytes J. Biol. Chem., October 21, 2005; 280(42): 35760 - 35766. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Lapidot, A. Dar, and O. Kollet How do stem cells find their way home? Blood, September 15, 2005; 106(6): 1901 - 1910. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Tavor, I. Petit, S. Porozov, P. Goichberg, A. Avigdor, S. Sagiv, A. Nagler, E. Naparstek, and T. Lapidot Motility, proliferation, and egress to the circulation of human AML cells are elastase dependent in NOD/SCID chimeric mice Blood, September 15, 2005; 106(6): 2120 - 2127. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Kucia, R. Reca, K. Miekus, J. Wanzeck, W. Wojakowski, A. Janowska-Wieczorek, J. Ratajczak, and M. Z. Ratajczak Trafficking of Normal Stem Cells and Metastasis of Cancer Stem Cells Involve Similar Mechanisms: Pivotal Role of the SDF-1-CXCR4 Axis Stem Cells, August 1, 2005; 23(7): 879 - 894. [Abstract] [Full Text] [PDF] |
||||
![]() |
P.-Y. Lozach, L. Burleigh, I. Staropoli, E. Navarro-Sanchez, J. Harriague, J.-L. Virelizier, F. A. Rey, P. Despres, F. Arenzana-Seisdedos, and A. Amara Dendritic Cell-specific Intercellular Adhesion Molecule 3-grabbing Non-integrin (DC-SIGN)-mediated Enhancement of Dengue Virus Infection Is Independent of DC-SIGN Internalization Signals J. Biol. Chem., June 24, 2005; 280(25): 23698 - 23708. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. A. Davis, K. E. Singer, M. De La Luz Sierra, M. Narazaki, F. Yang, H. M. Fales, R. Yarchoan, and G. Tosato Identification of carboxypeptidase N as an enzyme responsible for C-terminal cleavage of stromal cell-derived factor-1{alpha} in the circulation Blood, June 15, 2005; 105(12): 4561 - 4568. [Abstract] [Full Text] [PDF] |
||||
![]() |
J.-F. Geay, D. Buet, Y. Zhang, A. Foudi, P. Jarrier, M. Berthebaud, A. G. Turhan, W. Vainchenker, and F. Louache p210BCR-ABL inhibits SDF-1 Chemotactic Response via Alteration of CXCR4 Signaling and Down-regulation of CXCR4 Expression Cancer Res., April 1, 2005; 65(7): 2676 - 2683. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. Balabanian, B. Lagane, J. L. Pablos, L. Laurent, T. Planchenault, O. Verola, C. Lebbe, D. Kerob, A. Dupuy, O. Hermine, et al. WHIM syndromes with different genetic anomalies are accounted for by impaired CXCR4 desensitization to CXCL12 Blood, March 15, 2005; 105(6): 2449 - 2457. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Selleri, N. Montuori, P. Ricci, V. Visconte, M. V. Carriero, N. Sidenius, B. Serio, F. Blasi, B. Rotoli, G. Rossi, et al. Involvement of the urokinase-type plasminogen activator receptor in hematopoietic stem cell mobilization Blood, March 1, 2005; 105(5): 2198 - 2205. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. Sadir, A. Imberty, F. Baleux, and H. Lortat-Jacob Heparan Sulfate/Heparin Oligosaccharides Protect Stromal Cell-derived Factor-1 (SDF-1)/CXCL12 against Proteolysis Induced by CD26/Dipeptidyl Peptidase IV J. Biol. Chem., October 15, 2004; 279(42): 43854 - 43860. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. M. Rao, T. V. Betz, D. J. Lamont, M. B. Kim, S. K. Shaw, R. M. Froio, F. Baleux, F. Arenzana-Seisdedos, R. Alon, and F. W. Luscinskas Elastase Release by Transmigrating Neutrophils Deactivates Endothelial-bound SDF-1{alpha} and Attenuates Subsequent T Lymphocyte Transendothelial Migration J. Exp. Med., September 20, 2004; 200(6): 713 - 724. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. V. Gulino, D. Moratto, S. Sozzani, P. Cavadini, K. Otero, L. Tassone, L. Imberti, S. Pirovano, L. D. Notarangelo, R. Soresina, et al. Altered leukocyte response to CXCL12 in patients with warts hypogammaglobulinemia, infections, myelokathexis (WHIM) syndrome Blood, July 15, 2004; 104(2): 444 - 452. [Abstract] [Full Text] [PDF] |
||||
![]() |
B. T. Suratt, J. M. Petty, S. K. Young, K. C. Malcolm, J. G. Lieber, J. A. Nick, J.-A. Gonzalo, P. M. Henson, and G. S. Worthen Role of the CXCR4/SDF-1 chemokine axis in circulating neutrophil homeostasis Blood, July 15, 2004; 104(2): 565 - 571. [Abstract] [Full Text] [PDF] |
||||
![]() |
P. J. Hensbergen, D. Verzijl, C. I. A. Balog, R. Dijkman, R. C. van der Schors, E. M. H. van der Raaij-Helmer, M. J. A. van der Plas, R. Leurs, A. M. Deelder, M. J. Smit, et al. Furin Is a Chemokine-modifying Enzyme: IN VITRO AND IN VIVO PROCESSING OF CXCL10 GENERATES A C-TERMINALLY TRUNCATED CHEMOKINE RETAINING FULL ACTIVITY J. Biol. Chem., April 2, 2004; 279(14): 13402 - 13411. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Vroon, C. J. Heijnen, R. Raatgever, I. P. Touw, R. E. Ploemacher, R. T. Premont, and A. Kavelaars GRK6 deficiency is associated with enhanced CXCR4-mediated neutrophil chemotaxis in vitro and impaired responsiveness to G-CSF in vivo J. Leukoc. Biol., April 1, 2004; 75(4): 698 - 704. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. De La Luz Sierra, F. Yang, M. Narazaki, O. Salvucci, D. Davis, R. Yarchoan, H. H. Zhang, H. Fales, and G. Tosato Differential processing of stromal-derived factor-1{alpha} and stromal-derived factor-1{beta} explains functional diversity Blood, April 1, 2004; 103(7): 2452 - 2459. [Abstract] [Full Text] [PDF] |
||||
![]() |
F. E. Ouriaghli, E. Sloand, L. Mainwaring, H. Fujiwara, K. Keyvanfar, J. J. Melenhorst, K. Rezvani, G. Sconocchia, S. Solomon, N. Hensel, et al. Clonal dominance of chronic myelogenous leukemia is associated with diminished sensitivity to the antiproliferative effects of neutrophil elastase Blood, November 15, 2003; 102(10): 3786 - 3792. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Villalba, O. Salvucci, Y. Aoki, M. D. L. L. Sierra, G. Gupta, D. Davis, K. Wyvill, R. Little, R. Yarchoan, and G. Tosato Serum inactivation contributes to the failure of stromal-derived factor-1 to block HIV-I infection in vivo J. Leukoc. Biol., November 1, 2003; 74(5): 880 - 888. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. K. Stumm, C. Zhou, T. Ara, F. Lazarini, M. Dubois-Dalcq, T. Nagasawa, V. Hollt, and S. Schulz CXCR4 Regulates Interneuron Migration in the Developing Neocortex J. Neurosci., June 15, 2003; 23(12): 5123 - 5130. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Wolf, I. Clark-Lewis, C. Buri, H. Langen, M. Lis, and L. Mazzucchelli Cathepsin D Specifically Cleaves the Chemokines Macrophage Inflammatory Protein-1{alpha}, Macrophage Inflammatory Protein-1{beta}, and SLC That Are Expressed in Human Breast Cancer Am. J. Pathol., April 1, 2003; 162(4): 1183 - 1190. [Abstract] [Full Text] [PDF] |
||||
![]() |
O. Kollet, I. Petit, J. Kahn, S. Samira, A. Dar, A. Peled, V. Deutsch, M. Gunetti, W. Piacibello, A. Nagler, et al. Human CD34+CXCR4- sorted cells harbor intracellular CXCR4, which can be functionally expressed and provide NOD/SCID repopulation Blood, September 26, 2002; 100(8): 2778 - 2786. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |