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Originally published In Press as doi:10.1074/jbc.M201377200 on February 20, 2002
J. Biol. Chem., Vol. 277, Issue 18, 16139-16146, May 3, 2002
AUF1 Is a bcl-2 A + U-rich Element-binding
Protein Involved in bcl-2 mRNA
Destabilization during Apoptosis*
Andrea
Lapucci §¶,
Martino
Donnini §,
Laura
Papucci ,
Ewa
Witort ,
Alessio
Tempestini ,
Anna
Bevilacqua ,
Angelo
Nicolin ,
Gary
Brewer**,
Nicola
Schiavone  , and
Sergio
Capaccioli 
From the Department of Experimental Pathology and
Oncology, School of Medicine, University of Florence, 50134 Florence,
Italy, Department of Pharmacology, School of Medicine,
University of Milan, 20129 Milan, Italy, and ** Department of
Molecular Genetics and Microbiology, University of Medicine and
Dentistry of New Jersey, Robert Wood Johnson Medical School,
Piscataway, New Jersey 08854
We previously identified a conserved A + U-rich
element (ARE) in the 3'-untranslated region of bcl-2
mRNA. We have also recently demonstrated that the bcl-2
ARE interacts with a number of ARE-binding proteins (AUBPs) whose
pattern changes during apoptosis in association with bcl-2
mRNA half-life reduction. Here we show that the AUBP AUF1 binds
in vitro to bcl-2 mRNA. The results
obtained in a yeast RNA three-hybrid system have demonstrated that the
1-257-amino acid portion of p37 AUF1 (conserved in all isoforms),
containing the two RNA recognition motifs, also binds to the
bcl-2 ARE in vivo. UVC irradiation-induced
apoptosis results in an increase of AUF1. Inhibition of apoptosis by a
general caspase inhibitor reduces this increase by 2-3-fold.
These results indicate involvement of AUF1 in the
ARE/AUBP-mediated modulation of bcl-2 mRNA decay during apoptosis.
*
This work was supported by grants from the Associazione
Italiana Ricerca sul Cancro, Ministero dell'Università e Ricerca Scientifica e Tecnologica, and Ente Cassa di Risparmio di Firenze (to
S. C.) and National Institutes of Health Grant CA 52443 (to G. B.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
§
Both authors contributed equally to this work.
¶
Recipient of a fellowship from the Federazione Italiana
Ricerca Cancro.

To whom correspondence may be addressed: Dept. of Experimental
Pathology and Oncology, School of Medicine, University of Florence, Viale G.B. Morgagni 50, 50134 Florence, Italy. Tel.: 390-55-4282309; Fax: 390-55-4282333; E-mail: sergio@unifi.it (S. C.) or
nicola{at}unifi.it (N. S.).
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.

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Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.
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