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J. Biol. Chem., Vol. 277, Issue 18, 16211-16219, May 3, 2002

This Article Full Text Full Text (PDF) All Versions of this Article: 277/18/16211    most recent M106556200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Foley, H. A. Articles by Pace, B. S. Search for Related Content PubMed PubMed Citation Articles by Foley, H. A. Articles by Pace, B. S. Social Bookmarking                      What's this?

Stat3 Inhibits -Globin Gene Expression in Erythroid Cells^*

Heather A. Foley, Solomon F. Ofori-Acquah, Akihiko Yoshimura^§, Stuart Critz, B. Surendra Baliga^¶, and Betty S. Pace^¶

From the  Department of Cell Biology and Neuroscience, University of South Alabama, Mobile, Alabama 36688, the ^§ Department of Molecular and Cellular Immunology, Kyushu University, Fukuoka, Japan, and the ^¶ Department of Pediatrics, University of South Alabama, Mobile, Alabama 36606

We demonstrated previously -globin gene inhibition in K562 cells and primary erythroid progenitors treated with interleukin-6. Although several cis-acting elements have been identified in the globin promoters, the precise mechanism for cytokine-mediated globin gene regulation remains to be elucidated. In this report we demonstrate inhibitors of Stat3 phosphorylation abrogate interleukin-6-mediated  gene silencing in erythroid cells. DNA-protein binding studies established Stat3 interaction in the 5'-untranslated -globin promoter region. Furthermore, co-transfection experiments with Stat3 demonstrate  promoter inhibition in a concentration-dependent manner, which was significantly reversed when the cognate Stat3-binding site in the 5'-untranslated region was mutated. These studies establish a novel mechanism for  gene silencing through the STAT signal transduction pathway.

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