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J. Biol. Chem., Vol. 277, Issue 18, 16211-16219, May 3, 2002

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Stat3 Inhibits -Globin Gene Expression in Erythroid Cells^*

Heather A. Foley, Solomon F. Ofori-Acquah, Akihiko Yoshimura^§, Stuart Critz, B. Surendra Baliga^¶, and Betty S. Pace^¶

From the  Department of Cell Biology and Neuroscience, University of South Alabama, Mobile, Alabama 36688, the ^§ Department of Molecular and Cellular Immunology, Kyushu University, Fukuoka, Japan, and the ^¶ Department of Pediatrics, University of South Alabama, Mobile, Alabama 36606

We demonstrated previously -globin gene inhibition in K562 cells and primary erythroid progenitors treated with interleukin-6. Although several cis-acting elements have been identified in the globin promoters, the precise mechanism for cytokine-mediated globin gene regulation remains to be elucidated. In this report we demonstrate inhibitors of Stat3 phosphorylation abrogate interleukin-6-mediated  gene silencing in erythroid cells. DNA-protein binding studies established Stat3 interaction in the 5'-untranslated -globin promoter region. Furthermore, co-transfection experiments with Stat3 demonstrate  promoter inhibition in a concentration-dependent manner, which was significantly reversed when the cognate Stat3-binding site in the 5'-untranslated region was mutated. These studies establish a novel mechanism for  gene silencing through the STAT signal transduction pathway.

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