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J. Biol. Chem., Vol. 277, Issue 19, 16553-16558, May 10, 2002
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From the Molecular Genetics Department, National Institute of
Agrobiological Sciences, Tsukuba, Ibaraki 305-8602 and the Core
Research for Evolutional Science and Technology, Japan Science and
Technology Corporation, Ochanomizu, Chiyoda-ku, Tokyo 101-0062, Japan
E2F transcription factors are major regulators of
cell proliferation, and each factor contributes differently to cell
cycle control. Arabidopsis contains six E2F homologs, of
which three are proteins that exhibit an overall similarity to animal
E2Fs and interact with DPa and DPb to stimulate DNA binding. Here we describe the other three E2F-like proteins from
Arabidopsis, E2L1-3, which have two copies of a domain
with a limited similarity only to the DNA binding domain of E2F. Unlike
known E2Fs, the three E2L proteins failed to interact with DPa and DPb
and could efficiently bind E2F sites in a monomeric form through the
dual-type domain. Transfection assays revealed that E2Ls repress the
transcription of reporter genes under the control of E2F-regulated
promoters, indicating that E2Ls function to antagonize transactivation
mediated by E2F·DP. When fused to green fluorescence protein, E2L1
and E2L3 were predominantly localized to the nucleus whereas E2L2 was
detected in both the nucleus and cytoplasm. Because the transcripts of
E2Ls were abundant in meristematic rather than fully differentiated tissues, E2Ls may balance the activities of E2F·DP and play a role in
restraining cell proliferation.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AB074531, AB074532, AB074533.
To whom correspondence should be addressed. Tel.: 81-298-38-7440;
Fax: 81-298-38-7469; E-mail: yohashi@affrc.go.jp.
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