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J. Biol. Chem., Vol. 277, Issue 19, 17315-17319, May 10, 2002

This Article Full Text Full Text (PDF) All Versions of this Article: 277/19/17315    most recent M200901200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hsu, Y. Y. Articles by Wang, Y.-H. Search for Related Content PubMed PubMed Citation Articles by Hsu, Y. Y. Articles by Wang, Y.-H. Social Bookmarking                      What's this?

Human Fragile Site FRA16B DNA Excludes Nucleosomes in the Presence of Distamycin^*

Ying Ying Hsu and Yuh-Hwa Wang

From the Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854

Human fragile sites are weak staining gaps in chromosomes generated by specific culture conditions. The short CGG repeating DNA derived from folate-sensitive fragile sites has been shown to exclude single nucleosomes. To test whether this nucleosome exclusion model provides a general molecular mechanism for the formation of fragile sites, a different class of fragile site, the 33-base pair AT-rich repeating DNAs derived from the rare distamycin-inducible site, FRA16B, was examined for its ability to assemble single nucleosomes and nucleosome arrays using in vitro nucleosome reconstitution methods. The FRA16B DNA fragments strongly exclude nucleosome assembly only in the presence of distamycin, and increasing the number of 33-bp repeats increases the effect of distamycin in the destabilization of the nucleosome formation, suggesting a common mechanism for the formation of fragile sites.

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