|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 19, 17320-17326, May 10, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the The intracellular trafficking processes
controlling phagosomal maturation remain to be fully delineated.
Mycobacterium tuberculosis var. bovis BCG, an
organism that causes phagosomal maturation arrest, has emerged as a
tool for dissection of critical phagosome biogenesis events. In this
work, we report that cellubrevin, a v-SNARE functioning in endosomal
recycling and implicated in endosomal interactions with post-Golgi
compartments, plays a role in phagosomal maturation and that it is
altered on mycobacterial phagosomes. Both mycobacterial phagosomes,
which undergo maturation arrest, and model phagosomes containing latex
beads, which follow the normal pathway of maturation into
phagolysosomes, acquired cellubrevin. However, the mycobacterial and
model phagosomes differed, as a discrete proteolytic degradation of
this SNARE was detected on mycobacterial phagosomes. The observed
cellubrevin alteration on mycobacterial phagosomes was not a passive
event secondary to a maturation arrest at another checkpoint of the
phagosome maturation pathway, since pharmacological inhibitors of
phagosomal/endosomal pathways blocking phagosomal maturation did not
cause cellubrevin degradation on model phagosomes. Cellubrevin status
on phagosomes had consequences on phagosomal membrane and lumenal
content trafficking, involving plasma membrane marker recycling and
delivery of lysosomal enzymes. These results suggest that cellubrevin
plays a role in phagosomal maturation and that it is a target for
modification by mycobacteria or by infection-induced processes in the
host cell.
Cellubrevin Alterations and Mycobacterium
tuberculosis Phagosome Maturation Arrest*
§,§, and
**
Department of Microbiology and Immunology
and ¶ Program in Cellular and Molecular Biology, University of
Michigan Medical School, Ann Arbor, Michigan and the Departments
of § Molecular Genetics and Microbiology and
Cell Biology and Physiology, University of New Mexico School of
Medicine, Albuquerque, New Mexico 87131
*
This work was supported by National Institutes of Health
Grant AI45148.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Quach, N. M. van Sorge, S. A. Kristian, J. D. Bryan, D. W. Shelver, and K. S. Doran The CiaR Response Regulator in Group B Streptococcus Promotes Intracellular Survival and Resistance to Innate Immune Defenses J. Bacteriol., April 1, 2009; 191(7): 2023 - 2032. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Firoved, W. Ornatowski, and V. Deretic Microarray Analysis Reveals Induction of Lipoprotein Genes in Mucoid Pseudomonas aeruginosa: Implications for Inflammation in Cystic Fibrosis Infect. Immun., September 1, 2004; 72(9): 5012 - 5018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Douglas, D. S. Daniel, B. K. Parida, C. Jagannath, and S. Dhandayuthapani Methionine Sulfoxide Reductase A (MsrA) Deficiency Affects the Survival of Mycobacterium smegmatis within Macrophages J. Bacteriol., June 1, 2004; 186(11): 3590 - 3598. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. V. Vieira, R. E. Harrison, C. C. Scott, H. Stenmark, D. Alexander, J. Liu, J. Gruenberg, A. D. Schreiber, and S. Grinstein Acquisition of Hrs, an Essential Component of Phagosomal Maturation, Is Impaired by Mycobacteria Mol. Cell. Biol., May 15, 2004; 24(10): 4593 - 4604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. H. Miller, R. A. Fratti, J. F. Poschet, G. S. Timmins, S. S. Master, M. Burgos, M. A. Marletta, and V. Deretic Mycobacteria Inhibit Nitric Oxide Synthase Recruitment to Phagosomes during Macrophage Infection Infect. Immun., May 1, 2004; 72(5): 2872 - 2878. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Vergne, J. Chua, and V. Deretic Tuberculosis Toxin Blocking Phagosome Maturation Inhibits a Novel Ca2+/Calmodulin-PI3K hVPS34 Cascade J. Exp. Med., August 18, 2003; 198(4): 653 - 659. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Indrigo, R. L. Hunter Jr, and J. K. Actor Cord factor trehalose 6,6'-dimycolate (TDM) mediates trafficking events during mycobacterial infection of murine macrophages Microbiology, August 1, 2003; 149(8): 2049 - 2059. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. A. Kelley and J. S. Schorey Mycobacterium's Arrest of Phagosome Maturation in Macrophages Requires Rab5 Activity and Accessibility to Iron Mol. Biol. Cell, August 1, 2003; 14(8): 3366 - 3377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Solomon, G. S. Leung, and R. R. Isberg Intracellular Replication of Mycobacterium marinum within Dictyostelium discoideum: Efficient Replication in the Absence of Host Coronin Infect. Immun., June 1, 2003; 71(6): 3578 - 3586. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Fratti, J. Chua, I. Vergne, and V. Deretic Mycobacterium tuberculosis glycosylated phosphatidylinositol causes phagosome maturation arrest PNAS, April 29, 2003; 100(9): 5437 - 5442. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. A. Malik, C. R. Thompson, S. Hashimi, B. Porter, S. S. Iyer, and D. J. Kusner Cutting Edge: Mycobacterium tuberculosis Blocks Ca2+ Signaling and Phagosome Maturation in Human Macrophages Via Specific Inhibition of Sphingosine Kinase J. Immunol., March 15, 2003; 170(6): 2811 - 2815. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Tailleux, O. Neyrolles, S. Honore-Bouakline, E. Perret, F. Sanchez, J.-P. Abastado, P. H. Lagrange, J. C. Gluckman, M. Rosenzwajg, and J.-L. Herrmann Constrained Intracellular Survival of Mycobacterium tuberculosis in Human Dendritic Cells J. Immunol., February 15, 2003; 170(4): 1939 - 1948. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. Russell, H. C. Mwandumba, and E. E. Rhoades Mycobacterium and the coat of many lipids J. Cell Biol., August 5, 2002; 158(3): 421 - 426. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |