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J. Biol. Chem., Vol. 277, Issue 2, 1066-1075, January 11, 2002

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The mRNA of DEAD Box Protein p72 Is Alternatively Translated into an 82-kDa RNA Helicase^*

Heike Uhlmann-Schiffler, Oliver G. Rössler, and Hans Stahl

From the Fachbereich Medizinische Biochemie und Molekularbiologie, Fachrichtung Theoretische Medizin, Universität des Saarlandes, D-66421 Homburg, Germany

p68 and p72 are two highly related DEAD box proteins with similar biochemical activities in the nucleus of vertebrate cells; it is unknown whether they have redundant or differential in vivo functions. We report on a third member of this subfamily that is alternatively expressed from p72 mRNA. A detailed analysis of HeLa p72 mRNA was performed. It has an overall length of more than 5 kb and contains a 0.75-kb 5'-untranslated region and a 3'-untranslated region of 2.5 kb. Its open reading frame extends to nucleotide 243 upstream of the first in-frame AUG (A in the AUG triplet is +1) which serves as the p72 translation initiator codon. We provide evidence that alternative translation at a non-AUG within the extra coding region of this mRNA yields an 82-kDa protein (p82). Immunological studies substantiate that p82 is a naturally existing p72 variant and that both proteins are expressed at similar concentrations. p82 purified from HeLa cells is an ATP-dependent RNA helicase with biochemical properties almost identical to those of p72.

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