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J. Biol. Chem., Vol. 277, Issue 2, 1120-1127, January 11, 2002

This Article Full Text Full Text (PDF) All Versions of this Article: 277/2/1120    most recent M109912200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Boras, K. Articles by Hamel, P. A. Search for Related Content PubMed PubMed Citation Articles by Boras, K. Articles by Hamel, P. A. Social Bookmarking                      What's this?

Alx4 Binding to LEF-1 Regulates N-CAM Promoter Activity^*

Kata Boras and Paul A. Hamel

From the Department of Laboratory Medicine and Pathobiology, University of Toronto, Ontario M5S 1A8, Canada

During murine embryogenesis, expression of the paired-like homeodomain protein Alx4 is restricted to tissues whose development depends on the expression of lymphoid enhancer factor-1 (LEF-1). Given the defects seen in hair follicle development in both LEF-1 and Alx4 knockout and mutant animals and the overlapping expression patterns, we predicted that LEF-1 and Alx4 might form physical complexes. We demonstrate here the interaction between LEF-1 and Alx4. This interaction is mediated through a specific proline-rich domain in the N-terminal region of Alx4 and requires the DNA-binding domain (HMG-box) of LEF-1. We also demonstrate that LEF-1 and Alx4 can bind simultaneously to adjacent sites on the neural cell adhesion molecule (N-CAM) promoter and that this binding alters N-CAM promoter activity. Furthermore, when expressed in primary mammary stromal cells, Alx4 decreases the expression of endogenous N-CAM protein. These results reveal a potential mechanism that gives rise to mesenchymal-specific activities of LEF-1.

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